gadX [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Official Symbol: gadX
Primary source: ECOCYC:EG12243
Locus tag: b3516
See related: ASAP:ABE-0011487
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: ECK3501; yhiX
Summary: The GadX (glutamate decarboxylase regulator) transcriptional activator, which belongs to the AraC/XylS family , is a monomer of 31 kDa that can form a homodimer in vivo [12446650]. [More information is available at EcoCyc: EG12243].
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Genomic context

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Sequence:: NC_000913.3 (3664986..3665810, complement)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

These results show how GadEWX simultaneously coordinate many cellular processes to produce the overall response of E. coli to acid stress.
Title: Decoding genome-wide GadEWX-transcriptional regulatory networks reveals multifaceted cellular responses to acid stress in Escherichia coli.
Through biological and biochemical analysis, authors have demonstrated the GadX can directly interact with btuB promoter and affect the expression of btuB.
Title: Repression of btuB gene transcription in Escherichia coli by the GadX protein.
The authors found that the overexpression of the GadX and GadY regulators of the glutamate-dependent acid resistance system complemented the growth defect of YidC-depleted cells.
Title: Activators of the glutamate-dependent acid resistance system alleviate deleterious effects of YidC depletion in Escherichia coli.
gadX is transcribed in an operon with gadW, although this full-length mRNA does not accumulate.
Title: RNase III participates in GadY-dependent cleavage of the gadX-gadW mRNA.
GadX/GadW-dependent activation of the acid fitness island is controlled at the gadX-gadW intergenic region, in which the gadY gene is located and transcribed divergently from gadW.
Title: GadX/GadW-dependent regulation of the Escherichia coli acid fitness island: transcriptional control at the gadY-gadW divergent promoters and identification of four novel 42 bp GadX/GadW-specific binding sites.
The results indicate that the GadX regulator increases multidrug resistance in E. coli by activating the MdtEF multidrug efflux pump
Title: The AraC-family regulator GadX enhances multidrug resistance in Escherichia coli by activating expression of mdtEF multidrug efflux genes.
The effects of sodium on the acid resistance system of GadA/BC expression appears to be through the modulation of GadX and GadW activity, which in turn controls GadE expression.
Title: Sodium regulates Escherichia coli acid resistance, and influences GadX- and GadW-dependent activation of gadE.
The AraC-family regulators GadX and GadW, previously found to activate gadA/BC in vitro, are now shown in vivo to directly activate gadE expression, which, in turn, activates the gadA/BC genes.
Title: The Escherichia coli AraC-family regulators GadX and GadW activate gadE, the central activator of glutamate-dependent acid resistance.
H-NS directly inhibits gadA and gadX transcription and, by controlling the intracellular level of the activator GadX, indirectly affects the expression of the whole gad system
Title: Antagonistic role of H-NS and GadX in the regulation of the glutamate decarboxylase-dependent acid resistance system in Escherichia coli.

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Pathways from PubChem

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General gene information

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Gene Ontology Provided by EcoCyc

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA-binding transcription activator activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables DNA-binding transcription factor activity	IBA Inferred from Biological aspect of Ancestor more info
enables DNA-binding transcription factor activity	IEA Inferred from Electronic Annotation more info
enables cis-regulatory region sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables protein homodimerization activity	IDA Inferred from Direct Assay more info	PubMed
enables sequence-specific DNA binding	IEA Inferred from Electronic Annotation more info
enables transcription cis-regulatory region binding	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within DNA damage response	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within DNA-templated transcription	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within DNA-templated transcription	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within positive regulation of DNA-templated transcription	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of DNA-templated transcription	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: DNA-binding transcriptional dual regulator GadX

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_000913.3 Reference assembly

Range

3664986..3665810 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NP_417973.1 DNA-binding transcriptional dual regulator GadX [Escherichia coli str. K-12 substr. MG1655]

See identical proteins and their annotated locations for NP_417973.1

Status: PROVISIONAL

UniProtKB/TrEMBL

A0A5C9ACD4, A0A6L7CPL9

Conserved Domains (1) summary

PRK09978
Location:1 → 274

PRK09978; DNA-binding transcriptional regulator GadX; Provisional