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nfuA iron-sulfur cluster carrier protein NfuA [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 947925, updated on 26-Mar-2025
Official Symbol
nfuA
Official Full Name
iron-sulfur cluster carrier protein NfuA
Primary source
ECOCYC:G7748
Locus tag
b3414
See related
ASAP:ABE-0011144
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK3401; gntY; yhgI
Summary
NfuA can transfer its [4Fe-4S] cluster into apo-aconitase AcnA and nfuA mutants have reduced survival to oxidative and iron stress (Angelini, 2008). nfuA(yhgI) is required for the utilization of DNA as a carbon source and is a H. influenzae competence protein ComG homolog (Palchevskiy, 2006). nfuA(gntY) does not have a role in gluconate metabolism (T.Conway, personal communication, 2000). iscA, sufA, erpA and nfuA are paralogs, but GntY has an additional thioredoxin-like C-terminal domain. [More information is available at EcoGene: EG12935]. NfuA is an iron-sulfur protein which is able to transfer its [4Fe-4S] iron-sulfur cluster to apo-aconitase in vitro, thus identifying it as an iron-sulfur cluster scaffold protein . [More information is available at EcoCyc: G7748].
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Sequence:
NC_000913.3 (3545624..3546199)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene pimeloyl-acyl carrier protein methyl ester esterase Neighboring gene DNA utilization protein YhgH Neighboring gene high-affinity gluconate transporter Neighboring gene 4-alpha-glucanotransferase

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Escherichia coli nfuA is essential for maintenance of Shiga toxin phage Min27 lysogeny under iron-depleted condition. Cao D, et al. FEMS Microbiol Lett, 2015 Oct. PMID 26337151
  2. Molecular organization, biochemical function, cellular role and evolution of NfuA, an atypical Fe-S carrier. Py B, et al. Mol Microbiol, 2012 Oct. PMID 22966982
  3. Destruction and reformation of an iron-sulfur cluster during catalysis by lipoyl synthase. McCarthy EL, et al. Science, 2017 Oct 20. PMID 29051382, Free PMC Article
  4. Physical and functional interactions of a monothiol glutaredoxin and an iron sulfur cluster carrier protein with the sulfur-donating radical S-adenosyl-L-methionine enzyme MiaB. Boutigny S, et al. J Biol Chem, 2013 May 17. PMID 23543739, Free PMC Article
  5. DNA as a nutrient: novel role for bacterial competence gene homologs. Finkel SE, et al. J Bacteriol, 2001 Nov. PMID 11591672, Free PMC Article

See all (17) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. E. coli K12 MG1655 and its lysogenic strain carrying Stx2-encoding PhiMin27 followed by gene deletion, we provided clear evidence here that NfuA plays an important role in Fe metabolism and maintenance of Stx prophagy lysogeny.
    Title: Escherichia coli nfuA is essential for maintenance of Shiga toxin phage Min27 lysogeny under iron-depleted condition.
  2. GrxD and NfuA could play complementary roles in maturation and repair of MiaB
    Title: Physical and functional interactions of a monothiol glutaredoxin and an iron sulfur cluster carrier protein with the sulfur-donating radical S-adenosyl-L-methionine enzyme MiaB.
  3. In vivo, NfuA is necessary for full activity of complex I under aerobic growth conditions, and of AcnB in the presence of superoxide.
    Title: Molecular organization, biochemical function, cellular role and evolution of NfuA, an atypical Fe-S carrier.
Submit: New GeneRIF Correction
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables 4 iron, 4 sulfur cluster binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables 4 iron, 4 sulfur cluster binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables 4 iron, 4 sulfur cluster binding IEA
Inferred from Electronic Annotation
more info
  
enables iron ion binding IEA
Inferred from Electronic Annotation
more info
  
enables iron-sulfur cluster binding IEA
Inferred from Electronic Annotation
more info
  
enables iron-sulfur cluster chaperone activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein homodimerization activity IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
acts_upstream_of_or_within carbon utilization IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within cellular response to iron ion starvation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within iron-sulfur cluster assembly IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in iron-sulfur cluster assembly IEA
Inferred from Electronic Annotation
more info
  
involved_in protein maturation IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in protein maturation IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within response to oxidative stress IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
Preferred Names
iron-sulfur cluster carrier protein NfuA

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    3545624..3546199
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_417873.1 iron-sulfur cluster carrier protein NfuA [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_417873.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A376MJW1
    Conserved Domains (1) summary
    PRK11190
    Location:1 → 191
    PRK11190; iron-sulfur cluster biogenesis protein NfuA
Nucleotide Protein
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