dam [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Official Symbol: dam
Primary source: ECOCYC:EG10204
Locus tag: b3387
See related: ASAP:ABE-0011063
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: ECK3374
Summary: Dam is a base flipping enzyme. [More information is available at EcoGene: EG10204]. DNA adenine methyltransferase (Dam), which is responsible for methylation of nearly all GATC sequences in Escherichia coli , is encoded by the dam gene and occurs at about 130 molecules per rapidly growing cell . [More information is available at EcoCyc: EG10204].
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Genomic context

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Sequence:: NC_000913.3 (3515077..3515913, complement)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

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Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

DNA adenine methylase, not the PstI restriction-modification system, regulates virulence gene expression in Shiga toxin-producing Escherichia coli.
Title: DNA adenine methylase, not the PstI restriction-modification system, regulates virulence gene expression in Shiga toxin-producing Escherichia coli.
Fur-Dam Regulatory Interplay at an Internal Promoter of the Enteroaggregative Escherichia coli Type VI Secretion sci1 Gene Cluster.
Title: Fur-Dam Regulatory Interplay at an Internal Promoter of the Enteroaggregative Escherichia coli Type VI Secretion sci1 Gene Cluster.
Structural analysis of a new Dam binding site on DNA with implications for methylation-independent transcriptional repression.
Title: Structures of Escherichia coli DNA adenine methyltransferase (Dam) in complex with a non-GATC sequence: potential implications for methylation-independent transcriptional repression.
We propose a model for intrasite methylation in which Dam bound to flanking DNA is an obligate intermediate.
Title: Proximal recognition sites facilitate intrasite hopping by DNA adenine methyltransferase: mechanistic exploration of epigenetic gene regulation.
Unlike other restriction-modification methyltransferases, E. coli DNA adenine methyltransferase carries out intrasite processive catalysis whereby the enzyme-DNA complex methylates both strands of unmethylated GATC site prior to dissociation from DNA.
Title: Escherichia coli DNA adenine methyltransferase: intrasite processivity and substrate-induced dimerization and activation.
only at low Lrp concentrations will Dam compete effectively for binding and methylation of the proximal GATC site, leading to a phase switch resulting in the expression of pili
Title: Competitive Lrp and Dam assembly at the pap regulatory region: implications for mechanisms of epigenetic regulation.
Lys(14) is important for EcoDam binding of the extrahelical target base into the active site pocket
Title: Two alternative conformations of S-adenosyl-L-homocysteine bound to Escherichia coli DNA adenine methyltransferase and the implication of conformational changes in regulating the catalytic cycle.
structural and functional analysis
Title: Functional characterization of Escherichia coli DNA adenine methyltransferase, a novel target for antibiotics.
the up-regulation of recombinational repair in dam mutants allows for the efficient repair of double-strand breaks whose formation is dependent on functional mismatch repair
Title: Analysis of global gene expression and double-strand-break formation in DNA adenine methyltransferase- and mismatch repair-deficient Escherichia coli.
DNA regions accessible to Dam methylase in transfected human cells apparently coincided with those hypersensitive to DNase I.
Title: Identification and mapping of open chromatin regions within a 140 kb polygenic locus of human chromosome 19 using E. coli Dam methylase.

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General gene information

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Gene Ontology Provided by EcoCyc

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables S-adenosyl-L-methionine binding	IBA Inferred from Biological aspect of Ancestor more info
enables S-adenosyl-L-methionine binding	IDA Inferred from Direct Assay more info	PubMed
enables methyltransferase activity	IEA Inferred from Electronic Annotation more info
enables nucleic acid binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables sequence-specific DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables site-specific DNA-methyltransferase (adenine-specific) activity	IBA Inferred from Biological aspect of Ancestor more info
enables site-specific DNA-methyltransferase (adenine-specific) activity	IDA Inferred from Direct Assay more info	PubMed
enables site-specific DNA-methyltransferase (adenine-specific) activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in DNA replication	IEA Inferred from Electronic Annotation more info
involved_in DNA restriction-modification system	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within DNA-templated DNA replication	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within bacterial-type DNA replication initiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in methylation	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within mismatch repair	EXP Inferred from Experiment more info	PubMed
involved_in mismatch repair	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within mismatch repair	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within mismatch repair	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to UV	IMP Inferred from Mutant Phenotype more info	PubMed

General protein information

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Preferred Names: DNA adenine methyltransferase

NP_417846.1

EC 2.1.1.72

NCBI Reference Sequences (RefSeq)

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Genome Annotation

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Reference assembly

Genomic

NC_000913.3 Reference assembly

Range

3515077..3515913 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NP_417846.1 DNA adenine methyltransferase [Escherichia coli str. K-12 substr. MG1655]

See identical proteins and their annotated locations for NP_417846.1

Status: PROVISIONAL

UniProtKB/TrEMBL

W1EVR9

Conserved Domains (1) summary

PRK10904
Location:1 → 271

PRK10904; adenine-specific DNA-methyltransferase