crp DNA-binding transcriptional dual regulator CRP [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Gene symbol: crp
Gene description: DNA-binding transcriptional dual regulator CRP
Primary source: EcoGene:EG10164
Locus tag: b3357
See related: ASAP:ABE-0010970; ECOCYC:EG10164
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: cap; csm; ECK3345; gurB
Summary: [More information is available at EcoCyc: EG10164].

Genomic context

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Sequence:: NC_000913.3 (3486120..3486752)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Escherichia coli deletion mutants lacking cAMP-receptor protein (CRP) maintains colony-forming activities even after a long period of starvation, while wild-type cells lost most of this activity. The lack of cAMP-CRP effectively retains high colony-forming activities, indicating that cAMP-CRP acts as a positive regulator necessary for the induction of the viable but non-culturable state in E. coli.
Title: cAMP-CRP acts as a key regulator for the viable but non-culturable state in Escherichia coli.
Data show that DNA-binding transcriptional dual regulators Cra and CRP are most active on poor carbon sources.
Title: Systems assessment of transcriptional regulation on central carbon metabolism by Cra and CRP.
Escherichia coli OxyS RNA triggers cephalothin resistance by modulating the expression of CRP-associated genes.
Title: Escherichia coli OxyS RNA triggers cephalothin resistance by modulating the expression of CRP-associated genes.
Study compares the ability of two statistical mechanical models of protein conformational change-to characterize the multi-step activation mechanism of the broadly acting CRP. These models have the predictive power to determine how combinations of mutations will interact, how double mutants will behave, and how each construct would regulate gene expression.
Title: Theoretical analysis of inducer and operator binding for cyclic-AMP receptor protein mutants.
Furthermore, we applied gene ontology annotation to reveal the biological functions of CRP-Feed-forward loops (FFLs).
Title: Large-Scale Functional Analysis of CRP-Mediated Feed-Forward Loops.
Crp was found to provide little to no protection of the DNA motif under activating conditions; instead, Crp demonstrated binding patterns that closely resembled those generated by sigma70; propose a hypothesis where Crp is absent from the DNA motif but remains associated with RNAP holoenzyme post-recruitment during transcription initiation
Title: ChIP-exo interrogation of Crp, DNA, and RNAP holoenzyme interactions.
cAMP and CRP influence E. coli biofilms through transcriptional regulation of csgD.
Title: The Catabolite Repressor Protein-Cyclic AMP Complex Regulates csgD and Biofilm Formation in Uropathogenic Escherichia coli.
Data indicate that cyclic AMP receptor protein (CRP) recombinant protein corresponding to its C-terminal DNA-binding domain (CDD) forms a dimer.
Title: C-terminal dimerization of apo-cyclic AMP receptor protein validated in solution.
Asymmetric configurations in a reengineered homodimer reveal multiple subunit communication pathways in protein allostery.
Title: Asymmetric configurations in a reengineered homodimer reveal multiple subunit communication pathways in protein allostery.
findings show that the cyclic AMP receptor protein (CRP) indirectly upregulates mqsRA transcription; also show that CRP plays an important role in antibiotic persistence, which seems to be partially mediated through MqsRA
Title: The cyclic AMP receptor protein (CRP) regulates mqsRA, coding for the bacterial toxin-antitoxin gene pair, in Escherichia coli.

Submit: New GeneRIF Correction See all GeneRIFs (60)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by GO

Function	Evidence Code	Pubs
DNA binding	IEA Inferred from Electronic Annotation more info
DNA-binding transcription factor activity	IBA Inferred from Biological aspect of Ancestor more info	PubMed
DNA-binding transcription factor activity	IEA Inferred from Electronic Annotation more info
cAMP binding	IEA Inferred from Electronic Annotation more info
identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
nucleotide binding	IEA Inferred from Electronic Annotation more info
protein binding	IPI Inferred from Physical Interaction more info	PubMed
sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
sequence-specific DNA binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
carbon catabolite repression of transcription	IMP Inferred from Mutant Phenotype more info	PubMed
negative regulation of transcription, DNA-templated	EXP Inferred from Experiment more info	PubMed
negative regulation of transcription, DNA-templated	IDA Inferred from Direct Assay more info	PubMed
positive regulation of transcription, DNA-templated	EXP Inferred from Experiment more info	PubMed
positive regulation of transcription, DNA-templated	IDA Inferred from Direct Assay more info	PubMed
regulation of transcription, DNA-templated	IEA Inferred from Electronic Annotation more info
transcription, DNA-templated	IDA Inferred from Direct Assay more info	PubMed
transcription, DNA-templated	IEP Inferred from Expression Pattern more info	PubMed
transcription, DNA-templated	IMP Inferred from Mutant Phenotype more info	PubMed
transcription, DNA-templated	ISS Inferred from Sequence or Structural Similarity more info	PubMed

Component	Evidence Code	Pubs
cytosol	IBA Inferred from Biological aspect of Ancestor more info	PubMed
cytosol	IDA Inferred from Direct Assay more info	PubMed
protein-DNA complex	IDA Inferred from Direct Assay more info	PubMed
protein-DNA complex	IPI Inferred from Physical Interaction more info	PubMed

General protein information

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Preferred Names: DNA-binding transcriptional dual regulator CRP

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_000913.3 Reference assembly

Range

3486120..3486752

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NP_417816.1 DNA-binding transcriptional dual regulator CRP [Escherichia coli str. K-12 substr. MG1655]

See identical proteins and their annotated locations for NP_417816.1

Status: PROVISIONAL

UniProtKB/Swiss-Prot

P0ACJ8

Conserved Domains (1) summary

PRK11753
Location:1 → 210

PRK11753; DNA-binding transcriptional dual regulator Crp; Provisional