tufA translation elongation factor Tu 1 [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Official Symbol: tufA
Official Full Name: translation elongation factor Tu 1
Primary source: ECOCYC:EG11036
Locus tag: b3339
See related: ASAP:ABE-0010912
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: ECK3326; kirT; pulT
Summary: Elongation factor Tu (EF-Tu) is the most abundant protein in Escherichia coli. [More information is available at EcoCyc: EG11036].
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Genomic context

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Sequence:: NC_000913.3 (3470145..3471329, complement)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Conformational changes in EF-Tu coordinate the rate-limiting passage of aa-tRNA through the accommodation corridor en route to the peptidyl transferase center of the large ribosomal subunit.
Title: Elongation factor-Tu can repetitively engage aminoacyl-tRNA within the ribosome during the proofreading stage of tRNA selection.
By analyzing incorporation of near- and non-cognate amino acids in a model protein EF-Tu, we show that most of missense errors are too rare to detect by conventional methods, such as DDA, and are estimated to be between <10-7-10-5 by QRAS.
Title: Broad range of missense error frequencies in cellular proteins.
Data show that tRNA, selenocysteine- (tRNASec) variant (tRNAUTuX) facilitates peptide elongation factor EF-Tu dependent selenocysteine (Sec) insertion in response to UAG codons.
Title: A synthetic tRNA for EF-Tu mediated selenocysteine incorporation in vivo and in vitro.
Disruption of the TF/DnaK chaperone pathway is rescued by overexpression of the redox-regulated chaperone Hsp33.
Title: Hsp33 controls elongation factor-Tu stability and allows Escherichia coli growth in the absence of the major DnaK and trigger factor chaperones.
EF-Tu in Qbeta replicase modulates RNA elongation processes in a distinct manner from its established function in protein synthesis.
Title: Molecular basis for RNA polymerization by Qβ replicase.
Data suggest that the identity of the esterified amino acid and three base pairs in the T stem of tRNA combine to define the affinity of each aa-tRNA for EF-Tu, both off and on the ribosome.
Title: Tuning the affinity of aminoacyl-tRNA to elongation factor Tu for optimal decoding.
N-terminus verified by Edman degradation on mature peptide
Title: Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12.
P-loop and domain movements have roles in EF-Tu for guanine nucleotide exchange
Title: The importance of P-loop and domain movements in EF-Tu for guanine nucleotide exchange.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by EcoCyc

Function	Evidence Code	Pubs
enables GTP binding	IEA Inferred from Electronic Annotation more info
enables GTPase activity	IEA Inferred from Electronic Annotation more info
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables guanosine tetraphosphate binding	IDA Inferred from Direct Assay more info	PubMed
enables guanosine tetraphosphate binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables translation elongation factor activity	IBA Inferred from Biological aspect of Ancestor more info
enables translation elongation factor activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in response to antibiotic	IEA Inferred from Electronic Annotation more info
involved_in translation	IEA Inferred from Electronic Annotation more info
involved_in translational elongation	IBA Inferred from Biological aspect of Ancestor more info
involved_in translational elongation	IEA Inferred from Electronic Annotation more info
involved_in translational elongation	NAS Non-traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	HDA more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
part_of guanyl-nucleotide exchange factor complex	IPI Inferred from Physical Interaction more info	PubMed
located_in plasma membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: translation elongation factor Tu 1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_000913.3 Reference assembly

Range

3470145..3471329 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NP_417798.1 translation elongation factor Tu 1 [Escherichia coli str. K-12 substr. MG1655]

See identical proteins and their annotated locations for NP_417798.1

Status: PROVISIONAL

UniProtKB/TrEMBL

A0A5B9ASQ8

Conserved Domains (1) summary

PRK00049
Location:1 → 393

PRK00049; elongation factor Tu; Reviewed