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rplB 50S ribosomal subunit protein L2 [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 947820, updated on 3-Dec-2024

Summary

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Official Symbol
rplB
Official Full Name
50S ribosomal subunit protein L2
Primary source
ECOCYC:EG10865
Locus tag
b3317
See related
ASAP:ABE-0010854
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK3304
Summary
The L2 protein is a component of the 50S subunit of the ribosome and is part of the peptidyltransferase center. [More information is available at EcoCyc: EG10865].
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Genomic context

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Sequence:
NC_000913.3 (3450543..3451364, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene 50S ribosomal subunit protein L22 Neighboring gene 30S ribosomal subunit protein S19 Neighboring gene 50S ribosomal subunit protein L23 Neighboring gene 50S ribosomal subunit protein L4 Neighboring gene 50S ribosomal subunit protein L3

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. The properties of the complex between ribosomal protein L2 and tRNA. Remme J, et al. FEBS Lett, 1985 Oct 14. PMID 2412896
  2. Functional genetic selection of Helix 66 in Escherichia coli 23S rRNA identified the eukaryotic-binding sequence for ribosomal protein L2. Kitahara K, et al. Nucleic Acids Res, 2007. PMID 17553838, Free PMC Article
  3. A ribosomal protein that is immunologically conserved in archaebacteria, eubacteria and eukaryotes. Schmid G, et al. FEBS Lett, 1984 Nov 19. PMID 6209167
  4. The Si-tag for immobilizing proteins on a silica surface. Taniguchi K, et al. Biotechnol Bioeng, 2007 Apr 15. PMID 17013933
  5. Ribosomal protein L2 is involved in the association of the ribosomal subunits, tRNA binding to A and P sites and peptidyl transfer. Diedrich G, et al. EMBO J, 2000 Oct 2. PMID 11013226, Free PMC Article

See all (40) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. A truncated form of L2 or the mature L2 physically interacts with the N-terminal region of DnaA to inhibit initiation from oriC by apparently interfering with DnaA oligomer formation.
    Title: Two forms of ribosomal protein L2 of Escherichia coli that inhibit DnaA in DNA replication.
  2. The direct interaction between RNAPalpha and RPL2 was confirmed both in vivo and in vitro by performing coimmunoprecipitation and bacterial two-hybrid experiments.
    Title: The ribosomal protein L2 interacts with the RNA polymerase alpha subunit and acts as a transcription modulator in Escherichia coli.
  3. N-terminus verified by Edman degradation on mature peptide
    Title: Structure of the Escherichia coli S10 ribosomal protein operon.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables RNA binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables RNA binding IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables rRNA binding IEA
Inferred from Electronic Annotation
more info
  
enables structural constituent of ribosome IBA
Inferred from Biological aspect of Ancestor
more info
  
enables structural constituent of ribosome IDA
Inferred from Direct Assay
more info
 PubMed 
enables structural constituent of ribosome IEA
Inferred from Electronic Annotation
more info
  
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
enables zinc ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in cytoplasmic translation IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in cytoplasmic translation NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in negative regulation of DNA-templated DNA replication initiation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in ribosomal large subunit assembly IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in translation IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
part_of DnaA-L2 complex IPI
Inferred from Physical Interaction
more info
 PubMed 
located_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytosol IEA
Inferred from Electronic Annotation
more info
  
part_of cytosolic large ribosomal subunit IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of cytosolic large ribosomal subunit IDA
Inferred from Direct Assay
more info
 PubMed 
part_of cytosolic large ribosomal subunit IPI
Inferred from Physical Interaction
more info
 PubMed 
part_of large ribosomal subunit IEA
Inferred from Electronic Annotation
more info
  
part_of ribonucleoprotein complex IEA
Inferred from Electronic Annotation
more info
  
located_in ribosome IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
50S ribosomal subunit protein L2

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    3450543..3451364 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_417776.1 50S ribosomal subunit protein L2 [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_417776.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A090B4C0, A0A7A7A9X5
    Conserved Domains (1) summary
    PRK09374
    Location:1 → 273
    rplB; 50S ribosomal protein L2; Validated
Nucleotide Protein
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