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rpsD 30S ribosomal subunit protein S4 [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 947793, updated on 30-Jul-2025
Official Symbol
rpsD
Official Full Name
30S ribosomal subunit protein S4
Primary source
ECOCYC:EG10903
Locus tag
b3296
See related
ASAP:ABE-0010805
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK3283; ramA; sud(2); sud2
Summary
The S4 protein, a component of the 30S subunit of the ribosome, functions in the assembly of the 30S ribosomal subunit, the mRNA helicase activity of the ribosome, the regulation of translation of a subset of ribosomal proteins, and transcription antitermination of rRNA operons. [More information is available at EcoCyc: EG10903].
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Sequence:
NC_000913.3 (3441055..3441675, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene 50S ribosomal subunit protein L17 Neighboring gene RNA polymerase subunit alpha Neighboring gene 30S ribosomal subunit protein S11 Neighboring gene 30S ribosomal subunit protein S13

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Localization of the binding site for protein S4 on 16 S ribosomal RNA by chemical and enzymatic probing and primer extension. Stern S, et al. J Mol Biol, 1986 Nov 5. PMID 3820298
  2. Determination of the complete amino-acid sequence of protein S4 from Escherichia coli ribosomes. Schiltz E, et al. Eur J Biochem, 1975 Aug 15. PMID 1100394
  3. Evidence for allosteric coupling between the ribosome and repressor binding sites of a translationally regulated mRNA. Tang CK, et al. Biochemistry, 1990 May 8. PMID 2112408
  4. S4-alpha mRNA translation repression complex. I. Thermodynamics of formation. Deckman IC, et al. J Mol Biol, 1987 Jul 20. PMID 2443719
  5. Escherichia coli cells bearing a ribosomal ambiguity mutation in rpsD have a mutator phenotype that correlates with increased mistranslation. Balashov S, et al. J Bacteriol, 2003 Aug. PMID 12897024, Free PMC Article

See all (71) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. A crayfish ALF inhibits the proliferation of microbiota by binding to RPS4 and MscL of E. coli.
    Title: A crayfish ALF inhibits the proliferation of microbiota by binding to RPS4 and MscL of E. coli.
  2. Structure-Based Mechanisms of a Molecular RNA Polymerase/Chaperone Machine Required for Ribosome Biosynthesis.
    Title: Structure-Based Mechanisms of a Molecular RNA Polymerase/Chaperone Machine Required for Ribosome Biosynthesis.
  3. 16S rRNA processing, 30S biogenesis and translational fidelity are all affected by C-terminal truncations in ribosomal protein uS4.
    Title: The C-terminus of ribosomal protein uS4 contributes to small ribosomal subunit biogenesis and the fidelity of translation.
  4. Single-molecule fluorescence resonance energy transfer (FRET) is used to observe real-time encounters between Escherichia coli ribosomal protein S4 and the 16S 5' domain RNA at an early stage of 30S assembly.
    Title: Protein-guided RNA dynamics during early ribosome assembly.
  5. The authors show that labile S4 complexes rearrange into stable complexes within a few minutes at 42 degrees C, with longer coincubation with 16S ribosomal RNA leading to an increased population of stable complexes.
    Title: Slow formation of stable complexes during coincubation of minimal rRNA and ribosomal protein S4.
  6. N-terminus verified by Edman degradation on mature peptide
    Title: The primary structure of ribosomal protein S4 from Escherichia coli.
Submit: New GeneRIF Correction
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables RNA binding IEA
Inferred from Electronic Annotation
more info
  
enables mRNA 5'-UTR binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables mRNA regulatory element binding translation repressor activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables rRNA binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables rRNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables rRNA binding IEA
Inferred from Electronic Annotation
more info
  
enables structural constituent of ribosome IBA
Inferred from Biological aspect of Ancestor
more info
  
enables structural constituent of ribosome IDA
Inferred from Direct Assay
more info
 PubMed 
enables structural constituent of ribosome IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in DNA-templated transcription termination IEA
Inferred from Electronic Annotation
more info
  
involved_in cytoplasmic translation NAS
Non-traceable Author Statement
more info
 PubMed 
acts_upstream_of_or_within maintenance of translational fidelity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within negative regulation of translational initiation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in regulation of translation IEA
Inferred from Electronic Annotation
more info
  
involved_in response to antibiotic IEA
Inferred from Electronic Annotation
more info
  
involved_in ribosomal small subunit assembly IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in ribosomal small subunit biogenesis IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in ribosome biogenesis IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within transcription antitermination IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in transcription antitermination IEA
Inferred from Electronic Annotation
more info
  
involved_in translation IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
part_of cytosolic small ribosomal subunit IDA
Inferred from Direct Assay
more info
 PubMed 
part_of cytosolic small ribosomal subunit IPI
Inferred from Physical Interaction
more info
 PubMed 
part_of ribonucleoprotein complex IEA
Inferred from Electronic Annotation
more info
  
located_in ribosome IEA
Inferred from Electronic Annotation
more info
  
part_of small ribosomal subunit IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of small ribosomal subunit IEA
Inferred from Electronic Annotation
more info
  
Preferred Names
30S ribosomal subunit protein S4

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    3441055..3441675 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_417755.1 30S ribosomal subunit protein S4

    See identical proteins and their annotated locations for NP_417755.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A8S0FJM6
    Conserved Domains (1) summary
    PRK05327
    Location:1 → 206
    rpsD; 30S ribosomal protein S4; Validated
Nucleotide Protein
Heading Accession and Version

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