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accC biotin carboxylase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 947761, updated on 23-Apr-2026
Official Symbol
accC
Official Full Name
biotin carboxylase
Primary source
ECOCYC:EG10276
Locus tag
b3256
See related
ASAP:ABE-0010677
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK3243; fabG
Summary
Mutations result in negative cooperativity with respect to the substrate bicarbonate. [More information is available at EcoGene: EG10276]. Mutations in the homologous and functionally identical subunit in mammalian proprionyl-CoA carboxylase and 3-methylcrotonyl-CoA carboxylase result in the metabolic deficiency diseases of propionic acidemia or methylcrotonylglycinuria. [More information is available at EcoCyc: EG10276].
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Sequence:
NC_000913.3 (3405917..3407266)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene pseudo Neighboring gene biotin carboxyl carrier protein Neighboring gene DUF997 domain-containing protein YhdT Neighboring gene pantothenate:Na(+) symporter Neighboring gene ribosomal protein L11 methyltransferase

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Modeling and numerical simulation of biotin carboxylase kinetics: implications for half-sites reactivity. de Queiroz MS, et al. J Theor Biol, 2007 May 7. PMID 17266990
  2. Kinetic characterization of mutations found in propionic acidemia and methylcrotonylglycinuria: evidence for cooperativity in biotin carboxylase. Sloane V, et al. J Biol Chem, 2004 Apr 16. PMID 14960587
  3. Function of Escherichia coli biotin carboxylase requires catalytic activity of both subunits of the homodimer. Janiyani K, et al. J Biol Chem, 2001 Aug 10. PMID 11390406
  4. Site-directed mutagenesis of ATP binding residues of biotin carboxylase. Insight into the mechanism of catalysis. Sloane V, et al. J Biol Chem, 2001 Jul 6. PMID 11346647
  5. Movement of the biotin carboxylase B-domain as a result of ATP binding. Thoden JB, et al. J Biol Chem, 2000 May 26. PMID 10821865

See all (57) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. For DnaK, acetyl-CoA carboxylase, biotin carboxylase subunit (AccC) and phosphate acetyltransferase (Pta) we also showed a direct role of PpiB in the functional control of these proteins because it increased the measured enzyme activity of each protein and further interfered with DnaK localization and the correct folding of AccC.
    Title: Cyclophilin PpiB is involved in motility and biofilm formation via its functional association with certain proteins.
  2. This study reports that increased levels of carboxyltransferase (CT) tetramer have no detectable effect on translation of the CT subunit mRNAs.
    Title: Evidence against translational repression by the carboxyltransferase component of Escherichia coli acetyl coenzyme A carboxylase.
  3. It was concluded that biotin carboxylase must be dimeric to fulfill its physiological function.
    Title: Dimerization of the bacterial biotin carboxylase subunit is required for acetyl coenzyme A carboxylase activity in vivo.
  4. analysis of biotin carboxylase in complex with substrates and implications for its catalytic mechanism
    Title: Crystal structure of biotin carboxylase in complex with substrates and implications for its catalytic mechanism.
  5. N-terminus verified by Edman degradation on complete protein
    Title: Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12.
  6. Results show that under AccC overexpression conditions AccB is underbiotinylated.
    Title: Coordinate expression of the acetyl coenzyme A carboxylase genes, accB and accC, is necessary for normal regulation of biotin synthesis in Escherichia coli.
Submit: New GeneRIF Correction
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables acetyl-CoA carboxylase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables biotin carboxylase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables biotin carboxylase activity IEA
Inferred from Electronic Annotation
more info
  
enables catalytic activity IEA
Inferred from Electronic Annotation
more info
  
enables ligase activity IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein homodimerization activity IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in fatty acid biosynthetic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in fatty acid biosynthetic process IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within fatty acid biosynthetic process IGI
Inferred from Genetic Interaction
more info
 PubMed 
acts_upstream_of_or_within fatty acid biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in malonyl-CoA biosynthetic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in malonyl-CoA biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within negative regulation of fatty acid biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in cytoplasm IC
Inferred by Curator
more info
 PubMed 
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
Preferred Names
biotin carboxylase
NP_417722.1

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    3405917..3407266
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_417722.1 biotin carboxylase

    See identical proteins and their annotated locations for NP_417722.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A418GHT7
    Conserved Domains (1) summary
    PRK08591
    Location:1 → 449
    PRK08591; acetyl-CoA carboxylase biotin carboxylase subunit; Validated
Nucleotide Protein
Heading Accession and Version

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