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ptsN phosphotransferase system enzyme IIA(Ntr) [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 947721, updated on 25-Jun-2025
Official Symbol
ptsN
Official Full Name
phosphotransferase system enzyme IIA(Ntr)
Primary source
ECOCYC:EG11682
Locus tag
b3204
See related
ASAP:ABE-0010520
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK3193; rpoP; yhbI
Summary
Although ptsN was predicted to have a sigma 28 (FliA) promoter, it can not be transcribed in vitro with sigma 28 holoenzyme (Yu, 2006). [More information is available at EcoGene: EG11682]. ptsN encodes a protein homologous to Enzyme IIAfru of the phosphoenolpyruvate (PEP)-dependent carbohydrate phosphotransferase system (PTS). [More information is available at EcoCyc: EG11682].
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Sequence:
NC_000913.3 (3346578..3347069)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene RNA polymerase sigma factor RpoN Neighboring gene ribosome hibernation-promoting factor Neighboring gene RNase adaptor protein RapZ Neighboring gene phosphorelay protein NPr Neighboring gene protein kinase-like domain-containing protein YrbL Neighboring gene peptidoglycan glycosyltransferase MtgA

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. The protein-protein interaction network of the Escherichia coli EIIA(Ntr) regulatory protein reveals a role in cell motility and metabolic control. Gravina F, et al. Res Microbiol, 2021 Nov-Dec. PMID 34563668
  2. 1H, 15N, and 13C chemical shift assignments of the Escherichia coli nitrogen regulatory phosphocarrier IIA(Ntr). Li X, et al. J Biomol NMR, 2003 Dec. PMID 14512741
  3. The three-dimensional structure of the nitrogen regulatory protein IIANtr from Escherichia coli. Bordo D, et al. J Mol Biol, 1998 May 29. PMID 9636714
  4. Proteome analysis of an Escherichia coli ptsN-null strain under different nitrogen regimes. Gravina F, et al. J Proteomics, 2018 Mar 1. PMID 29274402
  5. Growth Inhibition by External Potassium of Escherichia coli Lacking PtsN (EIIANtr) Is Caused by Potassium Limitation Mediated by YcgO. Sharma R, et al. J Bacteriol, 2016 Jul 1. PMID 27137496, Free PMC Article

See all (26) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. The protein-protein interaction network of the Escherichia coli EIIA(Ntr) regulatory protein reveals a role in cell motility and metabolic control.
    Title: The protein-protein interaction network of the Escherichia coli EIIA(Ntr) regulatory protein reveals a role in cell motility and metabolic control.
  2. ptsN is required for fitness under minimal medium and for the proper balance between RpoS and sigma 70 activities in such way that, in the absence of ptsN, RpoS-dependent genes are preferentially expressed.
    Title: Proteome analysis of an Escherichia coli ptsN-null strain under different nitrogen regimes.
  3. Overproduction of YcgO yields a potassium-sensitive phenotype with similarities to that displayed by the DeltaptsN mutation.
    Title: Growth Inhibition by External Potassium of Escherichia coli Lacking PtsN (EIIANtr) Is Caused by Potassium Limitation Mediated by YcgO.
  4. Glutamine and alpha-ketoglutarate regulate the phosphorylation state of the PTSNtr by direct effects on enzyme INtr autophosphorylation.
    Title: Reciprocal regulation of the autophosphorylation of enzyme INtr by glutamine and α-ketoglutarate in Escherichia coli.
  5. Here the authors show that all of the nutrient utilization phenotypes attributed to ptsN are manifested selectively in strains lacking functional ilvG.
    Title: Characteristic phenotypes associated with ptsN-null mutants in Escherichia coli K-12 are absent in strains with functional ilvG.
  6. PtsN controls sigma(70) and sigma(S) selectivity by regulating the intracellular K(+) level.
    Title: Potassium mediates Escherichia coli enzyme IIA(Ntr) -dependent regulation of sigma factor selectivity.
  7. Dephosphorylated IIANtr enhances activity of the kdp promoter by direct binding of dephosphorylated IIA(Ntr) to the sensor kinase KdpD.
    Title: Stimulation of the potassium sensor KdpD kinase activity by interaction with the phosphotransferase protein IIA(Ntr) in Escherichia coli.
  8. EIIANtr is readily phosphorylated at the histidine 73.
    Title: Requirements for the phosphorylation of the Escherichia coli EIIANtr protein in vivo.
  9. overexpression of ptsN reduces stress in the cell envelope, thereby promoting survival in the absence of sigma(E).
    Title: The extracytoplasmic stress factor, sigmaE, is required to maintain cell envelope integrity in Escherichia coli.
  10. The solution form of IIA(Ntr) from Escherichia coli and its interaction with its partner protein, NPr, were characterized by nuclear magnetic resonance spectroscopy.
    Title: NMR characterization of the Escherichia coli nitrogen regulatory protein IIANtr in solution and interaction with its partner protein, NPr.
Submit: New GeneRIF Correction See all GeneRIFs (11)
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables enzyme inhibitor activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein kinase activator activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables protein kinase activator activity IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
Preferred Names
phosphotransferase system enzyme IIA(Ntr)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    3346578..3347069
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_417671.1 phosphotransferase system enzyme IIA(Ntr) [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_417671.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A1X3IXV1, A0A1X3JDA0, A0AAJ3U1G2
    Conserved Domains (1) summary
    PRK10896
    Location:2 → 155
    PRK10896; PTS IIA-like nitrogen regulatory protein PtsN
Nucleotide Protein
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