ptsN [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Official Symbol: ptsN
Primary source: ECOCYC:EG11682
Locus tag: b3204
See related: ASAP:ABE-0010520
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: ECK3193; rpoP; yhbI
Summary: Although ptsN was predicted to have a sigma 28 (FliA) promoter, it can not be transcribed in vitro with sigma 28 holoenzyme (Yu, 2006). [More information is available at EcoGene: EG11682]. ptsN encodes a protein homologous to Enzyme IIAfru of the phosphoenolpyruvate (PEP)-dependent carbohydrate phosphotransferase system (PTS). [More information is available at EcoCyc: EG11682].
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Genomic context

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Sequence:: NC_000913.3 (3346578..3347069)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

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Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The protein-protein interaction network of the Escherichia coli EIIA(Ntr) regulatory protein reveals a role in cell motility and metabolic control.
Title: The protein-protein interaction network of the Escherichia coli EIIA(Ntr) regulatory protein reveals a role in cell motility and metabolic control.
ptsN is required for fitness under minimal medium and for the proper balance between RpoS and sigma 70 activities in such way that, in the absence of ptsN, RpoS-dependent genes are preferentially expressed.
Title: Proteome analysis of an Escherichia coli ptsN-null strain under different nitrogen regimes.
Overproduction of YcgO yields a potassium-sensitive phenotype with similarities to that displayed by the DeltaptsN mutation.
Title: Growth Inhibition by External Potassium of Escherichia coli Lacking PtsN (EIIANtr) Is Caused by Potassium Limitation Mediated by YcgO.
Glutamine and alpha-ketoglutarate regulate the phosphorylation state of the PTSNtr by direct effects on enzyme INtr autophosphorylation.
Title: Reciprocal regulation of the autophosphorylation of enzyme INtr by glutamine and α-ketoglutarate in Escherichia coli.
Here the authors show that all of the nutrient utilization phenotypes attributed to ptsN are manifested selectively in strains lacking functional ilvG.
Title: Characteristic phenotypes associated with ptsN-null mutants in Escherichia coli K-12 are absent in strains with functional ilvG.
PtsN controls sigma(70) and sigma(S) selectivity by regulating the intracellular K(+) level.
Title: Potassium mediates Escherichia coli enzyme IIA(Ntr) -dependent regulation of sigma factor selectivity.
Dephosphorylated IIANtr enhances activity of the kdp promoter by direct binding of dephosphorylated IIA(Ntr) to the sensor kinase KdpD.
Title: Stimulation of the potassium sensor KdpD kinase activity by interaction with the phosphotransferase protein IIA(Ntr) in Escherichia coli.
EIIANtr is readily phosphorylated at the histidine 73.
Title: Requirements for the phosphorylation of the Escherichia coli EIIANtr protein in vivo.
overexpression of ptsN reduces stress in the cell envelope, thereby promoting survival in the absence of sigma(E).
Title: The extracytoplasmic stress factor, sigmaE, is required to maintain cell envelope integrity in Escherichia coli.
The solution form of IIA(Ntr) from Escherichia coli and its interaction with its partner protein, NPr, were characterized by nuclear magnetic resonance spectroscopy.
Title: NMR characterization of the Escherichia coli nitrogen regulatory protein IIANtr in solution and interaction with its partner protein, NPr.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by EcoCyc

Function	Evidence Code	Pubs
enables enzyme inhibitor activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables kinase activity	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase activator activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein kinase activator activity	IDA Inferred from Direct Assay more info	PubMed
enables protein-N(PI)-phosphohistidine-sugar phosphotransferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in phosphoenolpyruvate-dependent sugar phosphotransferase system	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: phosphotransferase system enzyme IIA(Ntr)

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_000913.3 Reference assembly

Range

3346578..3347069

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NP_417671.1 phosphotransferase system enzyme IIA(Ntr) [Escherichia coli str. K-12 substr. MG1655]

See identical proteins and their annotated locations for NP_417671.1

Status: PROVISIONAL

UniProtKB/TrEMBL

A0A1X3I349, A0A1X3IXV1, A0A1X3JDA0, A0AAJ3U1G2

Conserved Domains (1) summary

PRK10896
Location:2 → 155

PRK10896; PTS IIA-like nitrogen regulatory protein PtsN