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mlaD intermembrane phospholipid transport system, substrate binding protein MlaD [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 947712, updated on 19-Nov-2025
Official Symbol
mlaD
Official Full Name
intermembrane phospholipid transport system, substrate binding protein MlaD
Primary source
ECOCYC:EG12799
Locus tag
b3193
See related
ASAP:ABE-0010493
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK3182; yrbD
Summary
The first 28 aa are predicted to be a Type I signal peptide. [More information is available at EcoGene: EG12799].
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Sequence:
NC_000913.3 (3337910..3338461, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene acid stress protein IbaG Neighboring gene intermembrane phospholipid transport system protein MlaB Neighboring gene intermembrane phospholipid transport system - periplasmic binding protein Neighboring gene intermembrane phospholipid transport system, integral membrane subunit MlaE Neighboring gene intermembrane phospholipid transport system, ATP binding subunit MlaF

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Structure of the MlaC-MlaD complex reveals molecular basis of periplasmic phospholipid transport. Wotherspoon P, et al. Nat Commun, 2024 Jul 30. PMID 39080293, Free PMC Article
  2. Structural insights into outer membrane asymmetry maintenance in Gram-negative bacteria by MlaFEDB. Tang X, et al. Nat Struct Mol Biol, 2021 Jan. PMID 33199922
  3. Unanticipated functional diversity among the TatA-type components of the Tat protein translocase. Eimer E, et al. Sci Rep, 2018 Jan 22. PMID 29358647, Free PMC Article
  4. Identification and functional analysis of 'hypothetical' genes expressed in Haemophilus influenzae. Kolker E, et al. Nucleic Acids Res, 2004. PMID 15121896, Free PMC Article
  5. Identification of two Shigella flexneri chromosomal loci involved in intercellular spreading. Hong M, et al. Infect Immun, 1998 Oct. PMID 9746567, Free PMC Article

See all (15) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Structure of the MlaC-MlaD complex reveals molecular basis of periplasmic phospholipid transport.
    Title: Structure of the MlaC-MlaD complex reveals molecular basis of periplasmic phospholipid transport.
  2. Conserved features of the MlaD domain aid the trafficking of hydrophobic molecules.
    Title: Conserved features of the MlaD domain aid the trafficking of hydrophobic molecules.
  3. Mutants defective in MCE domain proteins mlaD, pqiB and yebT were shown to have distinct but partially overlapping phenotypes, but the primary functions of PqiB and YebT differ from MlaD.
    Title: MCE domain proteins: conserved inner membrane lipid-binding proteins required for outer membrane homeostasis.
  4. The authors further demonstrate that MlaD forms extremely stable hexamers within the complex, functions in substrate binding with strong affinity for phospholipids, and modulates ATP hydrolytic activity. In addition, MlaB plays critical roles in both the assembly and activity of the MlaFEDB ATP-binding cassette transporter.
    Title: Defining key roles for auxiliary proteins in an ABC transporter that maintains bacterial outer membrane lipid asymmetry.
Submit: New GeneRIF Correction

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables phospholipid binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables phospholipid transfer activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables phospholipid transfer activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in intermembrane phospholipid transfer IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within intermembrane phospholipid transfer IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in phospholipid transport IEA
Inferred from Electronic Annotation
more info
  
involved_in phospholipid transport NAS
Non-traceable Author Statement
more info
 PubMed 
Component Evidence Code Pubs
located_in membrane NAS
Non-traceable Author Statement
more info
 PubMed 
part_of phospholipid-translocating ATPase complex IPI
Inferred from Physical Interaction
more info
 PubMed 
is_active_in plasma membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in plasma membrane IEA
Inferred from Electronic Annotation
more info
  
Preferred Names
intermembrane phospholipid transport system, substrate binding protein MlaD

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    3337910..3338461 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_417660.1 intermembrane phospholipid transport system, substrate binding protein MlaD

    See identical proteins and their annotated locations for NP_417660.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A8T5Y7E2
    Conserved Domains (1) summary
    TIGR04430
    Location:2 → 153
    OM_asym_MlaD; outer membrane lipid asymmetry maintenance protein MlaD
Nucleotide Protein
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Gene LinkOut

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