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obgE GTPase ObgE [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 947694, updated on 25-Jun-2025
Official Symbol
obgE
Official Full Name
GTPase ObgE
Primary source
ECOCYC:G7656
Locus tag
b3183
See related
ASAP:ABE-0010462
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
cgtA; ECK3172; obg; yhbZ
Summary
obgE mutants have lowered DnaA and elevated basal level ppGpp. obgE is required for phage lambda development. [More information is available at EcoGene: EG12795]. ObgE is an essential member of the Obg family of small GTP-binding proteins . [More information is available at EcoCyc: G7656].
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Sequence:
NC_000913.3 (3330582..3331754, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene transcription elongation factor GreA Neighboring gene peptidoglycan DD-endopeptidase DacB Neighboring gene putative transporter YhbE Neighboring gene 50S ribosomal subunit protein L27

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Involvement of the cgtA gene function in stimulation of DNA repair in Escherichia coli and Vibrio harveyi. Zielke R, et al. Microbiology (Reading), 2003 Jul. PMID 12855728
  2. Obg/CtgA, a signaling protein that controls replication, translation, and morphological development? Michel B. Dev Cell, 2005 Mar. PMID 15737924
  3. A Single-Amino-Acid Substitution in Obg Activates a New Programmed Cell Death Pathway in Escherichia coli. Dewachter L, et al. mBio, 2015 Dec 22. PMID 26695632, Free PMC Article
  4. Structural and functional insights into the mode of action of a universally conserved Obg GTPase. Feng B, et al. PLoS Biol, 2014 May. PMID 24844575, Free PMC Article
  5. Deficiency of essential GTP-binding protein ObgE in Escherichia coli inhibits chromosome partition. Kobayashi G, et al. Mol Microbiol, 2001 Sep. PMID 11555285

See all (38) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. YbiB: a novel interactor of the GTPase ObgE.
    Title: YbiB: a novel interactor of the GTPase ObgE.
  2. The data suggest that GDP or ppGpp binding is required but not sufficient for ObgE to activate expression of hokB, ultimately leading to persistence.
    Title: Biochemical determinants of ObgE-mediated persistence.
  3. Biophysical analysis, using small angle X-ray scattering and multi-angle light scattering experiments, revealed that ObgE is a monomer in solution, regardless of the bound nucleotide. In contrast to recent suggestions, our biochemical analyses further indicate that ObgE is neither activated by K(+) ions nor by homodimerization.
    Title: Structural and biochemical analysis of Escherichia coli ObgE, a central regulator of bacterial persistence.
  4. obgE is cotranscribed with ribosomal protein genes rplU and rpmA.
    Title: Coexpression of Escherichia coli obgE, Encoding the Evolutionarily Conserved Obg GTPase, with Ribosomal Proteins L21 and L27.
  5. The authors report that expression of a mutant isoform of the essential GTPase ObgE causes rapid loss of viability in Escherichia coli.
    Title: A Single-Amino-Acid Substitution in Obg Activates a New Programmed Cell Death Pathway in Escherichia coli.
  6. ObgE might act as a checkpoint in final stages of the 50S subunit assembly under normal growth conditions, and, as a (p)ppGpp effector, might also have a regulatory role in the production of the 50S subunit under stressed conditions.
    Title: Structural and functional insights into the mode of action of a universally conserved Obg GTPase.
  7. ObgE changes the ratio of ppGpp to pppGpp in the cell during a response to amino acid starvation.
    Title: The ObgE/CgtA GTPase influences the stringent response to amino acid starvation in Escherichia coli.
  8. In this study we apply a new semiquantitative proteomic approach to show that CgtA(E) is required for optimal incorporation of certain late-assembly ribosomal proteins into the large ribosomal subunit.
    Title: The Escherichia coli GTPase CgtAE is involved in late steps of large ribosome assembly.
  9. ObgE is required to license chromosome segregation and subsequent cell cycle events.
    Title: Chromosome segregation control by Escherichia coli ObgE GTPase.
  10. Study proposes that, in addition to its role as a 50S assembly factor, CgtA promotes SpoT (p)ppGpp degradation activity on the ribosome and that the loss of CgtA from the ribosome is necessary for maximal (p)ppGpp accumulation under stress conditions.
    Title: G-protein control of the ribosome-associated stress response protein SpoT.
Submit: New GeneRIF Correction
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables GDP binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables GTP binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables GTP binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables GTPase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables GTPase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables guanosine tetraphosphate binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables guanyl ribonucleotide binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables large ribosomal subunit rRNA binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables ribonucleoside triphosphate phosphatase activity IEA
Inferred from Electronic Annotation
more info
  
enables ribosomal large subunit binding IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
acts_upstream_of_or_within chromosome segregation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in dormancy process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in negative regulation of ribosome biogenesis IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in ribosomal large subunit assembly IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in ribosome assembly IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in ribosome biogenesis IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
Preferred Names
GTPase ObgE

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    3330582..3331754 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_417650.1 GTPase ObgE [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_417650.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A376M2B5
    Conserved Domains (1) summary
    PRK12298
    Location:1 → 390
    obgE; GTPase CgtA; Reviewed
Nucleotide Protein
Heading Accession and Version

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