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ebgA evolved beta-D-galactosidase subunit alpha [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 947583, updated on 6-Jul-2026
Official Symbol
ebgA
Official Full Name
evolved beta-D-galactosidase subunit alpha
Primary source
ECOCYC:EG10252
Locus tag
b3076
See related
ASAP:ABE-0010104
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK3066
Summary
Wildtype EbgA can be mutated to hydrolyze lactose. [More information is available at EcoGene: EG10252]. The EbgA-EbgC complex represents the second -D-galactosidase enzyme in Escherichia coli. [More information is available at EcoCyc: EG10252].
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Sequence:
NC_000913.3 (3222633..3225725)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene putative tRNA-binding protein YgjH Neighboring gene DNA-binding transcriptional repressor EbgR Neighboring gene DUF386 domain-containing evolved beta-D-galactosidase subunit beta Neighboring gene putative transporter YgjI Neighboring gene protein YgjJ

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Experimental evolution of a new enzymatic function. II. Evolution of multiple functions for ebg enzyme in E. coli. Hall BG. Genetics, 1978 Jul. PMID 97169, Free PMC Article
  2. Probable mechanism of enzyme evolution: how did EBG of E. coli originate? Erhan S. Med Hypotheses, 1979 Nov. PMID 119900
  3. Experimental evolution of a new enzymatic function. Kinetic analysis of the ancestral (ebg) and evolved (ebg) enzymes. Hall BG. J Mol Biol, 1976 Oct 15. PMID 794482
  4. High-throughput screening of enzyme libraries: in vitro evolution of a beta-galactosidase by fluorescence-activated sorting of double emulsions. Mastrobattista E, et al. Chem Biol, 2005 Dec. PMID 16356846
  5. Evolution of a new enzymatic function by recombination within a gene. Hall BG, et al. Proc Natl Acad Sci U S A, 1980 Jun. PMID 6774339, Free PMC Article

See all (32) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. N-terminus verified by Edman degradation on complete protein
    Title: Catalysis by the large subunit of the second beta-galactosidase of Escherichia coli in the absence of the small subunit.
Submit: New GeneRIF Correction
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables beta-galactosidase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables beta-galactosidase activity IEA
Inferred from Electronic Annotation
more info
  
enables carbohydrate binding IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity, hydrolyzing O-glycosyl compounds IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in carbohydrate metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in lactose catabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
Component Evidence Code Pubs
part_of beta-galactosidase complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of beta-galactosidase complex IEA
Inferred from Electronic Annotation
more info
  
Preferred Names
evolved beta-D-galactosidase subunit alpha

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    3222633..3225725
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. YP_026199.1 evolved beta-D-galactosidase subunit alpha

    See identical proteins and their annotated locations for YP_026199.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A0H2Z3B9
    Conserved Domains (1) summary
    PRK10340
    Location:1 → 1021
    ebgA; cryptic beta-D-galactosidase subunit alpha; Reviewed
Nucleotide Protein
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