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nuoK NADH:quinone oxidoreductase subunit K [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 947580, updated on 26-Mar-2025

Summary

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Official Symbol
nuoK
Official Full Name
NADH:quinone oxidoreductase subunit K
Primary source
ECOCYC:EG12091
Locus tag
b2279
See related
ASAP:ABE-0007534
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK2273
Summary
NuoK is part of the inner membrane component of NADH dehydrogenase I . [More information is available at EcoCyc: EG12091].
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Genomic context

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Sequence:
NC_000913.3 (2395043..2395345, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene NADH:quinone oxidoreductase subunit N Neighboring gene NADH:quinone oxidoreductase subunit M Neighboring gene NADH:quinone oxidoreductase subunit L Neighboring gene NADH:quinone oxidoreductase subunit J Neighboring gene NADH:quinone oxidoreductase subunit I Neighboring gene NADH:quinone oxidoreductase subunit H Neighboring gene NADH:quinone oxidoreductase subunit G

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Conserved negative charges in the transmembrane segments of subunit K of the NADH:ubiquinone oxidoreductase determine its dependence on YidC for membrane insertion. Price CE, et al. J Biol Chem, 2010 Feb 5. PMID 19959836, Free PMC Article
  2. The MELAS mutations 3946 and 3949 perturb the critical structure in a conserved loop of the ND1 subunit of mitochondrial complex I. Kervinen M, et al. Hum Mol Genet, 2006 Sep 1. PMID 16849371
  3. Electrostatics, hydration, and proton transfer dynamics in the membrane domain of respiratory complex I. Kaila VR, et al. Proc Natl Acad Sci U S A, 2014 May 13. PMID 24778264, Free PMC Article
  4. Mutagenesis of subunit N of the Escherichia coli complex I. Identification of the initiation codon and the sensitivity of mutants to decylubiquinone. Amarneh B, et al. Biochemistry, 2003 May 6. PMID 12718520
  5. Inhibition by capsaicin of NADH-quinone oxidoreductases is correlated with the presence of energy-coupling site 1 in various organisms. Yagi T. Arch Biochem Biophys, 1990 Sep. PMID 2118334

See all (77) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. We observe spontaneous hydration of a putative proton entry channel at the NuoN/K interface, which is sensitive to the protonation state of buried glutamic acid residues.
    Title: Electrostatics, hydration, and proton transfer dynamics in the membrane domain of respiratory complex I.
  2. Results suggest a model in which YidC serves as a membrane chaperone for the insertion of the less hydrophobic, negatively charged transmembrane segments of NuoK.
    Title: Conserved negative charges in the transmembrane segments of subunit K of the NADH:ubiquinone oxidoreductase determine its dependence on YidC for membrane insertion.
  3. Membrane-embedded conserved acidic residues glutamate-36 and glutamate-72 are important for the coupling mechanism of mitochondrial ND4L subunit NuoK of NADH-quinone oxidoreductase (NDH-1).
    Title: Characterization of the membrane domain subunit NuoK (ND4L) of the NADH-quinone oxidoreductase from Escherichia coli.
  4. The results provide new information on the function of the region affected by the MELAS mutations 3946 and 3949 that is not obtainable from patient samples or current eukaryote models.
    Title: The MELAS mutations 3946 and 3949 perturb the critical structure in a conserved loop of the ND1 subunit of mitochondrial complex I.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
contributes_to NADH dehydrogenase (ubiquinone) activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables NADH dehydrogenase (ubiquinone) activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
contributes_to NADH dehydrogenase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables NADH:ubiquinone reductase (non-electrogenic) activity IEA
Inferred from Electronic Annotation
more info
  
enables oxidoreductase activity, acting on NAD(P)H IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables quinone binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in ATP synthesis coupled electron transport IEA
Inferred from Electronic Annotation
more info
  
involved_in proton transmembrane transport IEA
Inferred from Electronic Annotation
more info
  
involved_in respiratory electron transport chain IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
part_of NADH dehydrogenase complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of NADH dehydrogenase complex IDA
Inferred from Direct Assay
more info
 PubMed 
located_in membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
located_in plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in plasma membrane IEA
Inferred from Electronic Annotation
more info
  
part_of respiratory chain complex I IDA
Inferred from Direct Assay
more info
 PubMed 
part_of respiratory chain complex I IMP
Inferred from Mutant Phenotype
more info
 PubMed 
part_of respiratory chain complex I NAS
Non-traceable Author Statement
more info
 PubMed 

General protein information

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Preferred Names
NADH:quinone oxidoreductase subunit K
NP_416782.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    2395043..2395345 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_416782.1 NADH:quinone oxidoreductase subunit K [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_416782.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A1V2GK77
    Conserved Domains (1) summary
    PRK05715
    Location:1 → 100
    PRK05715; NADH-quinone oxidoreductase subunit NuoK
Nucleotide Protein
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