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btuF vitamin B12 ABC transporter periplasmic binding protein [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 947574, updated on 6-Oct-2023

Summary

Gene symbol
btuF
Gene description
vitamin B12 ABC transporter periplasmic binding protein
Primary source
ASAP:ABE-0000542
Locus tag
b0158
See related
ECOCYC:EG12334
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK0157; yadT
Summary
Uncleaved precursor N-terminus was also sequenced, confirming this start site of translation. about 80% of the precursors had the initial Met cleaved. [More information is available at EcoGene: EG12334]. BtuF is the periplasmic vitamin B12 binding protein that delivers cobalamin to the ABC transporter BtuCD. [More information is available at EcoCyc: EG12334].
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Genomic context

Sequence:
NC_000913.3 (177662..178462, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene protein YadW Neighboring gene iron-sulfur cluster insertion protein ErpA Neighboring gene PF03458 family protein YadS Neighboring gene 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase Neighboring gene dGTP triphosphohydrolase Neighboring gene periplasmic serine endoprotease DegP

Bibliography

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables cobalamin binding IDA
Inferred from Direct Assay
more info
PubMed 
enables cobalamin binding IEA
Inferred from Electronic Annotation
more info
 
enables protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
Process Evidence Code Pubs
acts_upstream_of_or_within cobalamin transport IDA
Inferred from Direct Assay
more info
PubMed 
involved_in cobalamin transport IDA
Inferred from Direct Assay
more info
PubMed 
involved_in cobalamin transport IEA
Inferred from Electronic Annotation
more info
 
acts_upstream_of_or_within cobalamin transport IMP
Inferred from Mutant Phenotype
more info
PubMed 
Component Evidence Code Pubs
part_of cobalamin transport complex IPI
Inferred from Physical Interaction
more info
PubMed 
located_in membrane IDA
Inferred from Direct Assay
more info
PubMed 
located_in outer membrane-bounded periplasmic space IDA
Inferred from Direct Assay
more info
PubMed 
located_in periplasmic space IDA
Inferred from Direct Assay
more info
PubMed 
located_in periplasmic space IEA
Inferred from Electronic Annotation
more info
 

General protein information

Preferred Names
vitamin B12 ABC transporter periplasmic binding protein
NP_414700.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    177662..178462 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_414700.1 vitamin B12 ABC transporter periplasmic binding protein [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_414700.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A3L1NY60
    Conserved Domains (1) summary
    PRK03379
    Location:7266
    PRK03379; vitamin B12-transporter protein BtuF; Provisional