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hycI hydrogenase 3 maturation protease [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 947428, updated on 30-Jul-2025
Official Symbol
hycI
Official Full Name
hydrogenase 3 maturation protease
Primary source
ECOCYC:G7414
Locus tag
b2717
See related
ASAP:ABE-0008931
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK2712
Summary
FhlA regulon. [More information is available at EcoGene: EG13396]. HycI is a protease that cleaves the carboxy-terminus of HycE, modifying it into its final form as the large subunit of hydrogenase 3 . [More information is available at EcoCyc: G7414].
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Sequence:
NC_000913.3 (2842573..2843043, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene cryptic beta-glucoside specific PTS enzyme IIBC component Neighboring gene cryptic 6-phospho-beta-glucosidase AscB Neighboring gene formate hydrogenlyase assembly protein Neighboring gene hydrogenase 3 Neighboring gene hydrogenase 3 Neighboring gene hydrogenase 3 large subunit

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Activation of a [NiFe]-hydrogenase-4 isoenzyme by maturation proteases. Finney AJ, et al. Microbiology (Reading), 2020 Sep. PMID 32731905, Free PMC Article
  2. Crystal structure of hydrogenase maturating endopeptidase HycI from Escherichia coli. Kumarevel T, et al. Biochem Biophys Res Commun, 2009 Nov 13. PMID 19720045
  3. Solution structure and backbone dynamics of an endopeptidase HycI from Escherichia coli: implications for mechanism of the [NiFe] hydrogenase maturation. Yang F, et al. J Biol Chem, 2007 Feb 9. PMID 17150961
  4. Fidelity of metal insertion into hydrogenases. Magalon A, et al. FEBS Lett, 2001 Jun 15. PMID 11418115
  5. Analysis of the cleavage site specificity of the endopeptidase involved in the maturation of the large subunit of hydrogenase 3 from Escherichia coli. Theodoratou E, et al. Arch Microbiol, 2000 Feb. PMID 10795682

See all (16) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Activation of a [NiFe]-hydrogenase-4 isoenzyme by maturation proteases.
    Title: Activation of a [NiFe]-hydrogenase-4 isoenzyme by maturation proteases.
  2. the crystal structure of HycI at 1.70 A resolution was presented.
    Title: Crystal structure of hydrogenase maturating endopeptidase HycI from Escherichia coli.
  3. N-terminus verified by Edman degradation on mature peptide
    Title: Characterisation of a protease from Escherichia coli involved in hydrogenase maturation.
  4. First solution structure of M52 family peptidase HycI is reported; the backbone dynamics is well correlated with the structural and functional basis of substrate recognition.
    Title: Solution structure and backbone dynamics of an endopeptidase HycI from Escherichia coli: implications for mechanism of the [NiFe] hydrogenase maturation.
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Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables aspartic-type endopeptidase activity IEA
Inferred from Electronic Annotation
more info
  
enables endopeptidase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables endopeptidase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables enzyme activator activity IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables peptidase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in protein processing IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in protein processing IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in proteolysis IEA
Inferred from Electronic Annotation
more info
  
Preferred Names
hydrogenase 3 maturation protease

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    2842573..2843043 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_417197.1 hydrogenase 3 maturation protease

    See identical proteins and their annotated locations for NP_417197.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A8T5YBF7
    Conserved Domains (1) summary
    PRK11544
    Location:1 → 156
    hycI; hydrogenase 3 maturation protease; Provisional
Nucleotide Protein
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