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entE 2,3-dihydroxybenzoate-AMP ligase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 947426, updated on 23-Apr-2026
Official Symbol
entE
Official Full Name
2,3-dihydroxybenzoate-AMP ligase
Primary source
ECOCYC:EG10263
Locus tag
b0594
See related
ASAP:ABE-0002049
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK0587
Summary
Recent gel filtration data suggest that EntE may be a monomer not a dimer. [More information is available at EcoCyc: EG10263].
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Sequence:
NC_000913.3 (626070..627680)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene ferric enterobactin ABC transporter periplasmic binding protein Neighboring gene isochorismate synthase EntC Neighboring gene enterobactin synthase component B Neighboring gene 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase Neighboring gene proofreading thioesterase in enterobactin biosynthesis

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Studies on the enzymatic synthesis of enterochelin in Escherichia coli K-12. Four polypeptides involved in the conversion of 2,3-dihydroxybenzoate to enterochelin. Greenwood KT, et al. Biochim Biophys Acta, 1976 Dec 1. PMID 136989
  2. Enterobactin synthetase-catalyzed formation of P(1),P(3)-diadenosine-5'-tetraphosphate. Sikora AL, et al. Biochemistry, 2009 Nov 24. PMID 19852513, Free PMC Article
  3. Aryl acid adenylating enzymes involved in siderophore biosynthesis: fluorescence polarization assay, ligand specificity, and discovery of non-nucleoside inhibitors via high-throughput screening. Neres J, et al. Biochemistry, 2008 Nov 11. PMID 18928302, Free PMC Article
  4. Biosynthesis of enterochelin in Escherichia coli K-12: separation of the polypeptides coded for by the entD, E, F and G genes. Woodrow GC, et al. Biochim Biophys Acta, 1979 Jan 4. PMID 216414
  5. Studies on the enzymatic synthesis of the cyclic trimer of 2,3-dihydroxy-N-benzoyl-L-serine in Escherichia coli. Bryce GF, et al. Biochemistry, 1972 Apr 25. PMID 4337557

See all (33) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Functional analysis showed that the EntB-EntE interaction was surprisingly tolerant of a number of point mutations on the surface of EntB and EntE.
    Title: Structure of the EntB multidomain nonribosomal peptide synthetase and functional analysis of its interaction with the EntE adenylation domain.
Submit: New GeneRIF Correction
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables 2,3-dihydroxybenzoate--[aryl-carrier protein] ligase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables 2,3-dihydroxybenzoate--[aryl-carrier protein] ligase activity IEA
Inferred from Electronic Annotation
more info
  
enables 2,3-dihydroxybenzoate-serine ligase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables 2,3-dihydroxybenzoate-serine ligase activity IEA
Inferred from Electronic Annotation
more info
  
enables CoA-ligase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables acid-thiol ligase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables acid-thiol ligase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in enterobactin biosynthetic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in enterobactin biosynthetic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in enterobactin biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within enterobactin biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in siderophore biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
Preferred Names
2,3-dihydroxybenzoate-AMP ligase
NP_415126.1

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    626070..627680
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_415126.1 2,3-dihydroxybenzoate-AMP ligase

    See identical proteins and their annotated locations for NP_415126.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A8S7EGP4
    Conserved Domains (1) summary
    PRK10946
    Location:1 → 536
    entE; (2,3-dihydroxybenzoyl)adenylate synthase
Nucleotide Protein
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