csgB [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Official Symbol: csgB
Primary source: ECOCYC:G6547
Locus tag: b1041
See related: ASAP:ABE-0003534
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: ECK1027
Summary: The csgAB operon is regulated by CsgD. [More information is available at EcoGene: EG12621]. CsgB is the minor curlin subunit, which appears to function as a nucleator in the assembly of curli on the cell surface . [More information is available at EcoCyc: G6547].
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Genomic context

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Sequence:: NC_000913.3 (1103951..1104406)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

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Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Inactivation of the cysH gene results in increased surface adhesion and cell aggregation by activating the expression of the curli-encoding csgBAC operon via phosphoadenosine 59-phosphosulfate accumulation.
Title: Sulfate assimilation pathway intermediate phosphoadenosine 59-phosphosulfate acts as a signal molecule affecting production of curli fibres in Escherichia coli.
Constructed DeltatolC mutant and complement extraintestinal pathogenic E. coli (ExPEC) strains to study the role of TolC in the retention of biofilm formation and curli production capability under different osmotic conditions.
Title: TolC promotes ExPEC biofilm formation and curli production in response to medium osmolarity.
Substoichiometric concentrations of CsgB induce a change in the mechanism of CsgA aggregation from that of forming amorphous aggregates to that of structured intermediates similar to those of CsgB alone
Title: The E. coli CsgB nucleator of curli assembles to β-sheet oligomers that alter the CsgA fibrillization mechanism.
fibrils formed by CsgA and CsgB, the primary curli proteins of Escherichia coli, possess many of the hallmarks typical of amyloid
Title: The functional curli amyloid is not based on in-register parallel beta-sheet structure.
CsgD overexpression can overcome temperature-dependent control of the curli-encoding csgBA operon, but not of the cellulose-related adrA gene
Title: Cellulose modulates biofilm formation by counteracting curli-mediated colonization of solid surfaces in Escherichia coli.
CsgB(trunc) was only able to act as a nucleator when cells were genetically manipulated to secrete higher concentrations of CsgA
Title: The curli nucleator protein, CsgB, contains an amyloidogenic domain that directs CsgA polymerization.

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General gene information

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Gene Ontology Provided by EcoCyc

Function	Evidence Code	Pubs
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within amyloid fibril formation	IDA Inferred from Direct Assay more info	PubMed
involved_in amyloid fibril formation	IDA Inferred from Direct Assay more info	PubMed
involved_in cell adhesion	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within curli assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of amyloid fibril formation	EXP Inferred from Experiment more info	PubMed
involved_in regulation of amyloid fibril formation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within single-species biofilm formation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cell outer membrane	IDA Inferred from Direct Assay more info	PubMed
located_in pilus	IDA Inferred from Direct Assay more info	PubMed
located_in pilus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: curlin, minor subunit

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_000913.3 Reference assembly

Range

1103951..1104406

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NP_415559.1 curlin, minor subunit [Escherichia coli str. K-12 substr. MG1655]

See identical proteins and their annotated locations for NP_415559.1

Status: PROVISIONAL

UniProtKB/Swiss-Prot

P0ABK7

Conserved Domains (1) summary

PRK10101
Location:1 → 151

csgB; curlin minor subunit CsgB; Provisional