U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

recJ ssDNA-specific exonuclease RecJ [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 947367, updated on 30-Jul-2025
Official Symbol
recJ
Official Full Name
ssDNA-specific exonuclease RecJ
Primary source
ECOCYC:EG10830
Locus tag
b2892
See related
ASAP:ABE-0009492
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK2887
Summary
Lovett and Kolodner (1989) N-terminally sequenced RecJ, but the native RBS was not used to direct translation, so it has been removed from the Verified Set. [More information is available at EcoGene: EG10830]. Three essential activities for the initiation of recombination in the recB1080 mutant are provided by different proteins: helicase activity by RecB1080CD, 5' --> 3' exonuclease by RecJ, and RecA-single-stranded DNA filament formation by RecFOR. [More information is available at EcoCyc: EG10830].
Try the new Gene page
Try the new Transcripts and proteins table
Sequence:
NC_000913.3 (3036373..3038106, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene lysine--tRNA ligase Neighboring gene peptide chain release factor RF2 Neighboring gene protein disulfide isomerase DsbC Neighboring gene site-specific recombinase

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Genetic analysis of the recJ gene of Escherichia coli K-12. Lovett ST, et al. J Bacteriol, 1984 Jan. PMID 6317649, Free PMC Article
  2. RecFOR function is required for DNA repair and recombination in a RecA loading-deficient recB mutant of Escherichia coli. Ivancić-Baće I, et al. Genetics, 2003 Feb. PMID 12618388, Free PMC Article
  3. Role of the recJ gene product in UV-induced illegitimate recombination at the hotspot. Ukita T, et al. J Bacteriol, 1996 Apr. PMID 8636039, Free PMC Article
  4. Different effects of recJ and recN mutations on the postreplication repair of UV-damaged DNA in Escherichia coli K-12. Wang TC, et al. J Bacteriol, 1988 Jun. PMID 3286613, Free PMC Article
  5. Nascent DNA processing by RecJ favors lesion repair over translesion synthesis at arrested replication forks in Escherichia coli. Courcelle CT, et al. Proc Natl Acad Sci U S A, 2006 Jun 13. PMID 16754873, Free PMC Article

See all (57) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. UV-irradiated Escherichia coli cells with a recN mutation did not exhibit increased nucleoid condensation, but chromosome replication and cytokinesis occurred. In the uvrA and recJ strains, the condensation step was delayed compared to the wild-type strain, and chromosome replication and cytokinesis did not occur.
    Title: Influence of uvrA, recJ and recN gene mutations on nucleoid reorganization in UV-treated Escherichia coli cells.
  2. Data indicate that complementary action by RecQ helicase and RecJ nuclease permits initiation of recombinational repair from all double-stranded (dsDNA) ends.
    Title: RecQ helicase and RecJ nuclease provide complementary functions to resect DNA for homologous recombination.
  3. Study hypothesized that RecJ and XonA blunt the double-strand end (created by the replication fork reversal)in order to produce SOS(Con) expression.
    Title: RecA4142 causes SOS constitutive expression by loading onto reversed replication forks in Escherichia coli K-12.
  4. RecJ is needed to prepare ssDNA for subsequent loading of RecA protein. It implies that an additional type of RecA loading could exist in the cell
    Title: RecJ nuclease is required for SOS induction after introduction of a double-strand break in a RecA loading deficient recB mutant of Escherichia coli.
  5. Data show that UvrD and UvrD252 counteract RecQ, RecJ, and RecFOR at forks blocked in the rep mutant of Escherichia coli.
    Title: UvrD and UvrD252 counteract RecQ, RecJ, and RecFOR in a rep mutant of Escherichia coli.
  6. Data show that the RecJ nuclease and RecQ helicase, which partially degrade the nascent DNA at blocked replication forks, are required for the rapid recovery of DNA synthesis and prevent the potentially mutagenic bypass of UV lesions.
    Title: Nascent DNA processing by RecJ favors lesion repair over translesion synthesis at arrested replication forks in Escherichia coli.
  7. genetic analysis of recombination in a recB recD double mutant was performed; results show that conjugational recombination and DNA repair in this mutant are highly dependent on recJ, partially dependent on recFOR, and independent of recQ
    Title: Effects of recJ, recQ, and recFOR mutations on recombination in nuclease-deficient recB recD double mutants of Escherichia coli.
  8. Prebinding of single-stranded DNA in vitro by single-stranded DNA-binding protein does not inhibit RecJ degradation but, rather, enhances the activity of RecJ protein.
    Title: RecJ exonuclease: substrates, products and interaction with SSB.
Submit: New GeneRIF Correction
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables 5'-3' exonuclease activity IEA
Inferred from Electronic Annotation
more info
  
enables exonuclease activity IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables nuclease activity IEA
Inferred from Electronic Annotation
more info
  
enables nucleic acid binding IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables single-stranded DNA 5'-3' DNA exonuclease activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables single-stranded DNA 5'-3' DNA exonuclease activity IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in DNA recombination IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in DNA recombination IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within DNA recombination IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in DNA repair IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within DNA repair IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within response to X-ray IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Preferred Names
ssDNA-specific exonuclease RecJ
NP_417368.1

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    3036373..3038106 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_417368.1 ssDNA-specific exonuclease RecJ

    See identical proteins and their annotated locations for NP_417368.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A7L7EH15, A0A8S7EIK8
    Conserved Domains (1) summary
    PRK11070
    Location:3 → 577
    PRK11070; single-stranded-DNA-specific exonuclease RecJ
Nucleotide Protein
Heading Accession and Version

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.