recJ [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Official Symbol: recJ
Primary source: ECOCYC:EG10830
Locus tag: b2892
See related: ASAP:ABE-0009492
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: ECK2887
Summary: Lovett and Kolodner (1989) N-terminally sequenced RecJ, but the native RBS was not used to direct translation, so it has been removed from the Verified Set. [More information is available at EcoGene: EG10830]. Three essential activities for the initiation of recombination in the recB1080 mutant are provided by different proteins: helicase activity by RecB1080CD, 5' --> 3' exonuclease by RecJ, and RecA-single-stranded DNA filament formation by RecFOR. [More information is available at EcoCyc: EG10830].
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Genomic context

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Sequence:: NC_000913.3 (3036373..3038106, complement)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

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Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

UV-irradiated Escherichia coli cells with a recN mutation did not exhibit increased nucleoid condensation, but chromosome replication and cytokinesis occurred. In the uvrA and recJ strains, the condensation step was delayed compared to the wild-type strain, and chromosome replication and cytokinesis did not occur.
Title: Influence of uvrA, recJ and recN gene mutations on nucleoid reorganization in UV-treated Escherichia coli cells.
Data indicate that complementary action by RecQ helicase and RecJ nuclease permits initiation of recombinational repair from all double-stranded (dsDNA) ends.
Title: RecQ helicase and RecJ nuclease provide complementary functions to resect DNA for homologous recombination.
Study hypothesized that RecJ and XonA blunt the double-strand end (created by the replication fork reversal)in order to produce SOS(Con) expression.
Title: RecA4142 causes SOS constitutive expression by loading onto reversed replication forks in Escherichia coli K-12.
RecJ is needed to prepare ssDNA for subsequent loading of RecA protein. It implies that an additional type of RecA loading could exist in the cell
Title: RecJ nuclease is required for SOS induction after introduction of a double-strand break in a RecA loading deficient recB mutant of Escherichia coli.
Data show that UvrD and UvrD252 counteract RecQ, RecJ, and RecFOR at forks blocked in the rep mutant of Escherichia coli.
Title: UvrD and UvrD252 counteract RecQ, RecJ, and RecFOR in a rep mutant of Escherichia coli.
Data show that the RecJ nuclease and RecQ helicase, which partially degrade the nascent DNA at blocked replication forks, are required for the rapid recovery of DNA synthesis and prevent the potentially mutagenic bypass of UV lesions.
Title: Nascent DNA processing by RecJ favors lesion repair over translesion synthesis at arrested replication forks in Escherichia coli.
genetic analysis of recombination in a recB recD double mutant was performed; results show that conjugational recombination and DNA repair in this mutant are highly dependent on recJ, partially dependent on recFOR, and independent of recQ
Title: Effects of recJ, recQ, and recFOR mutations on recombination in nuclease-deficient recB recD double mutants of Escherichia coli.
Prebinding of single-stranded DNA in vitro by single-stranded DNA-binding protein does not inhibit RecJ degradation but, rather, enhances the activity of RecJ protein.
Title: RecJ exonuclease: substrates, products and interaction with SSB.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by EcoCyc

Function	Evidence Code	Pubs
enables 5'-3' exonuclease activity	IEA Inferred from Electronic Annotation more info
enables exonuclease activity	IEA Inferred from Electronic Annotation more info
enables nucleic acid binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables single-stranded DNA 5'-3' DNA exonuclease activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA recombination	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within DNA recombination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within DNA repair	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to X-ray	IMP Inferred from Mutant Phenotype more info	PubMed

General protein information

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Preferred Names: ssDNA-specific exonuclease RecJ

NP_417368.1

EC 3.1.11.6

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_000913.3 Reference assembly

Range

3036373..3038106 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NP_417368.1 ssDNA-specific exonuclease RecJ [Escherichia coli str. K-12 substr. MG1655]

See identical proteins and their annotated locations for NP_417368.1

Status: PROVISIONAL

UniProtKB/TrEMBL

A0A7L7EH15, A0A8S7EIK8

Conserved Domains (1) summary

PRK11070
Location:3 → 577

PRK11070; single-stranded-DNA-specific exonuclease RecJ