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hypA hydrogenase 3 Ni(2(+)) incorporation protein HypA [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 947195, updated on 17-Jul-2025
Official Symbol
hypA
Official Full Name
hydrogenase 3 Ni(2(+)) incorporation protein HypA
Primary source
ECOCYC:EG10483
Locus tag
b2726
See related
ASAP:ABE-0008958
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK2721
Summary
hypA mutants have increased Hydrogenase 2 activity but similar amounts of HypA protein. hypA defect suppressed by expression of hypB-E under foreign promoter, indicating a regulatory or partially redundant function. [More information is available at EcoGene: EG10483]. HypA and its homolog, HybF, are involved in the maturation of hydrogenase isozymes. [More information is available at EcoCyc: EG10483].
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Sequence:
NC_000913.3 (2850647..2850997)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene hydrogenase 3 membrane subunit HycC Neighboring gene hydrogenase 3 Neighboring gene regulator of the transcriptional regulator FhlA Neighboring gene hydrogenase isoenzymes Ni(2(+)) incorporation protein HypB Neighboring gene hydrogenase maturation factor HypC Neighboring gene Fe-(CN)2CO cofactor assembly scaffold protein HypD Neighboring gene carbamoyl dehydratase HypE

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Mechanism of Selective Nickel Transfer from HypB to HypA, Escherichia coli [NiFe]-Hydrogenase Accessory Proteins. Lacasse MJ, et al. Biochemistry, 2016 Dec 13. PMID 27951644
  2. Characterization of divergent NtrA-dependent promoters in the anaerobically expressed gene cluster coding for hydrogenase 3 components of Escherichia coli. Lutz S, et al. Mol Microbiol, 1990 Jan. PMID 2181234
  3. FNR-mediated regulation of hyp expression in Escherichia coli. Messenger SL, et al. FEMS Microbiol Lett, 2003 Nov 7. PMID 14612240
  4. Escherichia coli HypA is a zinc metalloprotein with a weak affinity for nickel. Atanassova A, et al. J Bacteriol, 2005 Jul. PMID 15995183, Free PMC Article
  5. Complex formation between the Escherichia coli [NiFe]-hydrogenase nickel maturation factors. Khorasani-Motlagh M, et al. Biometals, 2019 Jun. PMID 30758762

See all (18) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. At different stages of the hydrogenase maturation process, HypA was observed at or near the cell membrane, as was HycE, suggesting membrane localization of the nickel insertion event.
    Title: Protein interactions and localization of the Escherichia coli accessory protein HypA during nickel insertion to [NiFe] hydrogenase.
Submit: New GeneRIF Correction
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables identical protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables metallochaperone activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables nickel cation binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables nickel cation binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables zinc ion binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables zinc ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in protein maturation IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in protein maturation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in protein modification process IEA
Inferred from Electronic Annotation
more info
  
involved_in protein-containing complex assembly NAS
Non-traceable Author Statement
more info
 PubMed 
Component Evidence Code Pubs
part_of GTPase complex IPI
Inferred from Physical Interaction
more info
 PubMed 
part_of metallochaperone complex IPI
Inferred from Physical Interaction
more info
 PubMed 
located_in plasma membrane IEA
Inferred from Electronic Annotation
more info
  
Preferred Names
hydrogenase 3 Ni(2(+)) incorporation protein HypA

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    2850647..2850997
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_417206.1 hydrogenase 3 Ni(2(+)) incorporation protein HypA

    See identical proteins and their annotated locations for NP_417206.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A0H3ELG5, A0AAJ3NW56, B1LQ46
    Conserved Domains (1) summary
    PRK03681
    Location:1 → 114
    hypA; hydrogenase/urease nickel incorporation protein HypA
Nucleotide Protein
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