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alaE L-alanine exporter [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 947147, updated on 3-Dec-2024

Summary

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Official Symbol
alaE
Official Full Name
L-alanine exporter
Primary source
ECOCYC:G7399
Locus tag
b2670
See related
ASAP:ABE-0008793
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK2664; ygaW
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Genomic context

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Sequence:
NC_000913.3 (2799164..2799613)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene thiosulfate sulfurtransferase YgaP Neighboring gene DNA-binding transcriptional repressor StpA with RNA chaperone activity Neighboring gene DUF2002 domain-containing protein YgaC Neighboring gene DUF883 domain-containing protein YgaM

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Importance of transmembrane helix 4 of l-alanine exporter AlaE in oligomer formation and substrate export activity in Escherichia coli. Ihara K, et al. Microbiology (Reading), 2022 Mar. PMID 35275050
  2. L-Alanine Exporter, AlaE, of Escherichia coli Functions as a Safety Valve to Enhance Survival under Feast Conditions. Katsube S, et al. Int J Mol Sci, 2019 Oct 7. PMID 31591285, Free PMC Article
  3. Impact of charged amino acid substitution in the transmembrane domain of L-alanine exporter, AlaE, of Escherichia coli on the L-alanine export. Kim S, et al. Arch Microbiol, 2017 Jan. PMID 27572251
  4. Characterization of the l-alanine exporter AlaE of Escherichia coli and its potential role in protecting cells from a toxic-level accumulation of l-alanine and its derivatives. Kim S, et al. Microbiologyopen, 2015 Aug. PMID 26073055, Free PMC Article
  5. Expression of the alaE gene is positively regulated by the global regulator Lrp in response to intracellular accumulation of l-alanine in Escherichia coli. Ihara K, et al. J Biosci Bioeng, 2017 Apr. PMID 28057466

See all (13) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Importance of transmembrane helix 4 of l-alanine exporter AlaE in oligomer formation and substrate export activity in Escherichia coli.
    Title: Importance of transmembrane helix 4 of l-alanine exporter AlaE in oligomer formation and substrate export activity in Escherichia coli.
  2. Study provides evidence that the L-alanine exporter AlaE plays a critical role in avoiding a toxic accumulation of L-alanine and functions as the 'safety valve' under potential high L-alanine environments, such as in animal intestines.
    Title: L-Alanine Exporter, AlaE, of Escherichia coli Functions as a Safety Valve to Enhance Survival under Feast Conditions.
  3. the gene expression of the alaE is regulated by Lrp in response to intracellular levels of l-alanine, which eventually leads to intracellular homeostasis of l-alanine concentrations
    Title: Expression of the alaE gene is positively regulated by the global regulator Lrp in response to intracellular accumulation of l-alanine in Escherichia coli.
  4. Charged amino acid residues (R45 and D84) in the AlaE transmembrane domain play a pivotal role in L-alanine export.
    Title: Impact of charged amino acid substitution in the transmembrane domain of L-alanine exporter, AlaE, of Escherichia coli on the L-alanine export.
  5. These data suggested that the AlaE-mediated l-alanine extrusion was driven by proton motive force.
    Title: Characterization of the l-alanine exporter AlaE of Escherichia coli and its potential role in protecting cells from a toxic-level accumulation of l-alanine and its derivatives.
  6. On the basis of these results, the authors concluded that YgaW is likely to be the physiologically most relevant exporter for L-alanine in Escherichia coli and proposed that the gene be redesignated alaE for alanine export.
    Title: Inducible L-alanine exporter encoded by the novel gene ygaW (alaE) in Escherichia coli.
Submit: New GeneRIF Correction

General gene information

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Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables L-amino acid efflux transmembrane transporter activity EXP
Inferred from Experiment
more info
 PubMed 
enables L-amino acid efflux transmembrane transporter activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables L-amino acid efflux transmembrane transporter activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in L-alanine transport EXP
Inferred from Experiment
more info
 PubMed 
involved_in L-alanine transport IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in L-alpha-amino acid transmembrane transport IEA
Inferred from Electronic Annotation
more info
  
involved_in amino acid export across plasma membrane EXP
Inferred from Experiment
more info
 PubMed 
involved_in amino acid export across plasma membrane IEA
Inferred from Electronic Annotation
more info
  
involved_in amino acid transport IEA
Inferred from Electronic Annotation
more info
  
involved_in cellular response to stress IEP
Inferred from Expression Pattern
more info
 PubMed 
Component Evidence Code Pubs
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
located_in plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in plasma membrane IEA
Inferred from Electronic Annotation
more info
  
located_in plasma membrane RCA
inferred from Reviewed Computational Analysis
more info
 PubMed 

General protein information

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Preferred Names
L-alanine exporter

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    2799164..2799613
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_417156.1 L-alanine exporter [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_417156.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A7A6ZJY0, A0A7D7EUZ4
    Conserved Domains (1) summary
    pfam06610
    Location:3 → 141
    AlaE; L-alanine exporter
Nucleotide Protein
Heading Accession and Version

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