alaE L-alanine exporter [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Official Symbol: alaE
Official Full Name: L-alanine exporter
Primary source: ECOCYC:G7399
Locus tag: b2670
See related: ASAP:ABE-0008793
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: ECK2664; ygaW
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Genomic context

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Sequence:: NC_000913.3 (2799164..2799613)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Importance of transmembrane helix 4 of l-alanine exporter AlaE in oligomer formation and substrate export activity in Escherichia coli.
Title: Importance of transmembrane helix 4 of l-alanine exporter AlaE in oligomer formation and substrate export activity in Escherichia coli.
Study provides evidence that the L-alanine exporter AlaE plays a critical role in avoiding a toxic accumulation of L-alanine and functions as the 'safety valve' under potential high L-alanine environments, such as in animal intestines.
Title: L-Alanine Exporter, AlaE, of Escherichia coli Functions as a Safety Valve to Enhance Survival under Feast Conditions.
the gene expression of the alaE is regulated by Lrp in response to intracellular levels of l-alanine, which eventually leads to intracellular homeostasis of l-alanine concentrations
Title: Expression of the alaE gene is positively regulated by the global regulator Lrp in response to intracellular accumulation of l-alanine in Escherichia coli.
Charged amino acid residues (R45 and D84) in the AlaE transmembrane domain play a pivotal role in L-alanine export.
Title: Impact of charged amino acid substitution in the transmembrane domain of L-alanine exporter, AlaE, of Escherichia coli on the L-alanine export.
These data suggested that the AlaE-mediated l-alanine extrusion was driven by proton motive force.
Title: Characterization of the l-alanine exporter AlaE of Escherichia coli and its potential role in protecting cells from a toxic-level accumulation of l-alanine and its derivatives.
On the basis of these results, the authors concluded that YgaW is likely to be the physiologically most relevant exporter for L-alanine in Escherichia coli and proposed that the gene be redesignated alaE for alanine export.
Title: Inducible L-alanine exporter encoded by the novel gene ygaW (alaE) in Escherichia coli.

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General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by EcoCyc

Function	Evidence Code	Pubs
enables L-amino acid efflux transmembrane transporter activity	EXP Inferred from Experiment more info	PubMed
enables L-amino acid efflux transmembrane transporter activity	IDA Inferred from Direct Assay more info	PubMed
enables L-amino acid efflux transmembrane transporter activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in L-alanine transport	EXP Inferred from Experiment more info	PubMed
involved_in L-alanine transport	IDA Inferred from Direct Assay more info	PubMed
involved_in L-alpha-amino acid transmembrane transport	IEA Inferred from Electronic Annotation more info
involved_in amino acid export across plasma membrane	EXP Inferred from Experiment more info	PubMed
involved_in amino acid export across plasma membrane	IEA Inferred from Electronic Annotation more info
involved_in cellular response to stress	IEP Inferred from Expression Pattern more info	PubMed

Component	Evidence Code	Pubs
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	RCA inferred from Reviewed Computational Analysis more info	PubMed