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grcA stress-induced alternate pyruvate formate-lyase subunit [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 947068, updated on 19-Feb-2025
Official Symbol
grcA
Official Full Name
stress-induced alternate pyruvate formate-lyase subunit
Primary source
ECOCYC:EG11784
Locus tag
b2579
See related
ASAP:ABE-0008489
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK2577; yfiD
Summary
YfiD is required for MazF-mediated cell death after either DNA damage or inhibition of translation (Amitai, 2009). [More information is available at EcoGene: EG11784]. The yfiD gene encodes a glycyl radical protein that can replace an oxidatively damaged pyruvate formate-lyase subunit . [More information is available at EcoCyc: EG11784].
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Sequence:
NC_000913.3 (2716066..2716449, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene putative LysR-type DNA-binding transcriptional regulator YfiE Neighboring gene cysteine/O-acetylserine exporter EamB Neighboring gene uracil-DNA glycosylase Neighboring gene putative methyltransferase YfiF

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. HlyX, the FNR homologue of Actinobacillus pleuropneumoniae, is a [4Fe-4S]-containing oxygen-responsive transcription regulator that anaerobically activates FNR-dependent class I promoters via an enhanced AR1 contact. Green J, et al. Mol Microbiol, 1997 May. PMID 9179852
  2. Proteome analysis of metabolically engineered Escherichia coli producing Poly(3-hydroxybutyrate). Han MJ, et al. J Bacteriol, 2001 Jan. PMID 11114930, Free PMC Article
  3. Rescuing activity of oxygen-damaged pyruvate formate-lyase by a spare part protein. Andorfer MC, et al. J Biol Chem, 2021 Dec. PMID 34801558, Free PMC Article
  4. Downregulation of Escherichia coli yfiD expression by FNR occupying a site at -93.5 involves the AR1-containing face of FNR. Green J, et al. Mol Microbiol, 1998 Aug. PMID 9767578
  5. A novel promoter architecture for microaerobic activation by the anaerobic transcription factor FNR. Marshall FA, et al. Mol Microbiol, 2001 Feb. PMID 11169114

See all (34) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Rescuing activity of oxygen-damaged pyruvate formate-lyase by a spare part protein.
    Title: Rescuing activity of oxygen-damaged pyruvate formate-lyase by a spare part protein.
  2. N-terminus verified by Edman degradation on complete protein
    Title: Acid- and base-induced proteins during aerobic and anaerobic growth of Escherichia coli revealed by two-dimensional gel electrophoresis.
Submit: New GeneRIF Correction
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables catalytic activity IEA
Inferred from Electronic Annotation
more info
  
enables formate C-acetyltransferase activity EXP
Inferred from Experiment
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
acts_upstream_of_or_within response to stress IEP
Inferred from Expression Pattern
more info
 PubMed 
acts_upstream_of_or_within response to stress IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in cytoplasm HDA PubMed 
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
Preferred Names
stress-induced alternate pyruvate formate-lyase subunit

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    2716066..2716449 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_417074.1 stress-induced alternate pyruvate formate-lyase subunit [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_417074.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A192CAF1, A0AA36KZE6, Q1R8F3
    Conserved Domains (1) summary
    PRK11127
    Location:1 → 127
    PRK11127; autonomous glycyl radical cofactor GrcA; Provisional
Nucleotide Protein
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