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ung uracil-DNA glycosylase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 947067, updated on 30-Jul-2025
Official Symbol
ung
Official Full Name
uracil-DNA glycosylase
Primary source
ECOCYC:EG11058
Locus tag
b2580
See related
ASAP:ABE-0008495
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK2578
Summary
UDG and Ndk form a complex (Goswami, 2006). [More information is available at EcoGene: EG11058]. Uracil can be generated in DNA as a result of misincorporation by DNA polymerase, spontaneous deamination of cytosine, and deamination of cytosine induced by bisulfite or nitrous acid. [More information is available at EcoCyc: EG11058].
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Sequence:
NC_000913.3 (2716754..2717443)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene cysteine/O-acetylserine exporter EamB Neighboring gene stress-induced alternate pyruvate formate-lyase subunit Neighboring gene putative methyltransferase YfiF Neighboring gene thioredoxin 2

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Mutation of an active site residue in Escherichia coli uracil-DNA glycosylase: effect on DNA binding, uracil inhibition and catalysis. Shroyer MJ, et al. Biochemistry, 1999 Apr 13. PMID 10200172
  2. Effects of mutations at tyrosine 66 and asparagine 123 in the active site pocket of Escherichia coli uracil DNA glycosylase on uracil excision from synthetic DNA oligomers: evidence for the occurrence of long-range interactions between the enzyme and substrate. Handa P, et al. Nucleic Acids Res, 2002 Jul 15. PMID 12136091, Free PMC Article
  3. Presteady-state analysis of a single catalytic turnover by Escherichia coli uracil-DNA glycosylase reveals a "pinch-pull-push" mechanism. Wong I, et al. J Biol Chem, 2002 May 31. PMID 11907039
  4. Probing the limits of electrostatic catalysis by uracil DNA glycosylase using transition state mimicry and mutagenesis. Jiang YL, et al. J Biol Chem, 2002 May 3. PMID 11859082
  5. An exponential dilution gradient system for nanoscale liquid chromatography in combination with MALDI or nano-ESI mass spectrometry for proteolytic digests. Doneanu CE, et al. J Am Soc Mass Spectrom, 2001 Nov. PMID 11720396

See all (75) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. In vitro Evolution of Uracil Glycosylase Towards DnaKJ and GroEL Binding Evolves Different Misfolded States.
    Title: In vitro Evolution of Uracil Glycosylase Towards DnaKJ and GroEL Binding Evolves Different Misfolded States.
  2. A mutant uracil DNA glycosylase distinguishes between cytosine and 5-methylcytosine.
    Title: A mutant of uracil DNA glycosylase that distinguishes between cytosine and 5-methylcytosine.
  3. Contrasting effects of mutating active site residues, aspartic acid 64 and histidine 187 of Escherichia coli uracil-DNA glycosylase on uracil excision and interaction with an inhibitor protein
    Title: Contrasting effects of mutating active site residues, aspartic acid 64 and histidine 187 of Escherichia coli uracil-DNA glycosylase on uracil excision and interaction with an inhibitor protein.
  4. oligonucleotide substrates containing two uracil residues in defined positions were used to characterize one-dimensional search of DNA by Escherichia coli Ung.
    Title: Mechanism of translocation of uracil-DNA glycosylase from Escherichia coli between distributed lesions.
  5. The significance of the Ung residency on the AP-site in the uracil excision repair pathway in E. coli.
    Title: Analysis of the impact of a uracil DNA glycosylase attenuated in AP-DNA binding in maintenance of the genomic integrity in Escherichia coli.
  6. for the human and Escherichia coli UNG enzymes, discrimination of thymine and uracil is initiated by thermally induced opening of T*A and U*A base pairs and not by active participation of the enzyme
    Title: Enzymatic capture of an extrahelical thymine in the search for uracil in DNA.
  7. N-terminus verified by Edman degradation on mature peptide
    Title: Sequence analysis, expression, and conservation of Escherichia coli uracil DNA glycosylase and its gene (ung).
  8. influence of the ung, dug, and dut genes in preventing uracil residues from accumulating in E. coli DNA was quantitatively determined
    Title: Quantitative determination of uracil residues in Escherichia coli DNA: Contribution of ung, dug, and dut genes to uracil avoidance.
  9. A synthetic DNA duplex of uracil DNA glycosylase contains an excellent isostere of uracil but lacks hydrogen bonds and acceptor groups; it attains a metastable unstacked state that mimics a previously detected intermediate on the uracil-flipping pathway.
    Title: Recognition of an unnatural difluorophenyl nucleotide by uracil DNA glycosylase.
  10. The structure of native and modified uracil DNA glycosylase from E. coli in solution was studied by synchrotron small-angle X-ray scattering.
    Title: [Structure of Escherichia coli uracil DNA glycosylase and its complexes with nonhydrolyzable substrate analogues in solution observed by synchrotron small-angle X-ray scattering].
Submit: New GeneRIF Correction See all GeneRIFs (11)
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity, hydrolyzing N-glycosyl compounds IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables uracil DNA N-glycosylase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables uracil DNA N-glycosylase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables uracil DNA N-glycosylase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in DNA damage response IEA
Inferred from Electronic Annotation
more info
  
involved_in DNA repair IEA
Inferred from Electronic Annotation
more info
  
involved_in base-excision repair IEA
Inferred from Electronic Annotation
more info
  
involved_in base-excision repair, AP site formation via deaminated base removal IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in base-excision repair, AP site formation via deaminated base removal IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
Preferred Names
uracil-DNA glycosylase
NP_417075.1

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    2716754..2717443
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_417075.1 uracil-DNA glycosylase

    See identical proteins and their annotated locations for NP_417075.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    Q8FF08
    UniProtKB/TrEMBL
    A0A2X1N865, A0A5B9APQ3, A0A6C9QIE5
    Conserved Domains (2) summary
    PRK05254
    Location:4 → 220
    PRK05254; uracil-DNA glycosylase; Provisional
    cd10027
    Location:62 → 66
    UDG-F1-like; motif A [structural motif]
Nucleotide Protein
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