U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

rnc RNase III [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 947033, updated on 25-Jun-2025
Official Symbol
rnc
Official Full Name
RNase III
Primary source
ECOCYC:EG10857
Locus tag
b2567
See related
ASAP:ABE-0008448
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK2565
Summary
rnc is a non-essential gene (Takiff, 1989; Yu, 2000) and the failure to obtain a viable insertion (Baba, 2006) may be due to a polar effect on the downstream essential era gene. [More information is available at EcoGene: EG10857]. RNase III is an endonuclease that cleaves double-stranded RNA to yield 5'-phosphates and 3'-hydroxyls . [More information is available at EcoCyc: EG10857].
NEW
Try the new Gene table
Try the new Transcript table
Sequence:
NC_000913.3 (2703383..2704063, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene holo-[acyl-carrier-protein] synthase Neighboring gene pyridoxine 5'-phosphate synthase Neighboring gene recombination mediator protein RecO Neighboring gene 30S ribosomal subunit maturation GTPase Era Neighboring gene signal peptidase I Neighboring gene 30S ribosomal subunit biogenesis factor LepA

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Analysis of mRNA decay and rRNA processing in Escherichia coli multiple mutants carrying a deletion in RNase III. Babitzke P, et al. J Bacteriol, 1993 Jan. PMID 8416898, Free PMC Article
  2. RNase III cleavage of Escherichia coli beta-galactosidase and tryptophan operon mRNA. Shen V, et al. J Bacteriol, 1982 Jun. PMID 6176575, Free PMC Article
  3. The cII-independent expression of the phage lambda int gene in RNase III-defective E. coli. Belfort M. Gene, 1980 Oct. PMID 6254850
  4. Functional inactivation of lac alpha-peptide mRNA by a factor that purifies that Escherichia coli RNase III. Shen V, et al. J Biol Chem, 1981 Feb 25. PMID 6257691
  5. Regulation of plasmid DNA synthesis: isolation and characterization of copy number mutants of miniR6-5 and miniF plasmids. Ely S, et al. Mol Gen Genet, 1981. PMID 6261084

See all (152) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Uncovering a delicate balance between endonuclease RNase III and ribosomal protein S15 in E. coli ribosome assembly.
    Title: Uncovering a delicate balance between endonuclease RNase III and ribosomal protein S15 in E. coli ribosome assembly.
  2. Based on these findings, we generated a E38A/E65A/Q165A triple mutant of EcRNase III (EcEEQ) that functions as a bacterial Dicer and produces random, heterogeneous 22-nt-siRNA cocktails that are optimal for gene silencing studies
    Title: The molecular mechanism of dsRNA processing by a bacterial Dicer.
  3. In vivo RNA cleavage rules and target repertoire of RNase III in Escherichia coli have been reported.
    Title: In vivo cleavage rules and target repertoire of RNase III in Escherichia coli.
  4. Molecular dynamics simulations provide a mechanism for facilitated product release, in which the Ser33 phosphomonoester forms a salt bridge with the Arg95 guanidinium group, thereby weakening RNase III engagement of product.
    Title: Mechanism of Ribonuclease III Catalytic Regulation by Serine Phosphorylation.
  5. PNPase regulates its own expression via a reversible RNase III-independent pathway acting upstream from the RNase III-dependent branch.
    Title: RNase III-Independent Autogenous Regulation of Escherichia coli Polynucleotide Phosphorylase via Translational Repression.
  6. Authors propose that another major role for the Nus-modified transcription complex in rrn operons is as an RNA chaperone insuring co-ordination of 16S rRNA folding and RNase III processing that results in production of proper 30S ribosome subunits.
    Title: Nus transcription elongation factors and RNase III modulate small ribosome subunit biogenesis in Escherichia coli.
  7. The proU mRNA is processed by RNase III and this processing is osmoregulated.
    Title: RNase III initiates rapid degradation of proU mRNA upon hypo-osmotic stress in Escherichia coli.
  8. findings reveal an RNase III-mediated regulatory pathway that functions to modulate corA expression and, in turn, the influx of metal ions transported by CorA in E. coli.
    Title: RNase III controls the degradation of corA mRNA in Escherichia coli.
  9. Data indicates that RNase III has a more widespread role in cellular RNA processing than previously envisioned.
    Title: Analysis of Escherichia coli RNase E and RNase III activity in vivo using tiling microarrays.
  10. Multiple enzymes are involved in the GadY-directed cleavage of the full-length mRNA including the double-strand RNA-specific endoribonuclease RNase III.
    Title: RNase III participates in GadY-dependent cleavage of the gadX-gadW mRNA.
Submit: New GeneRIF Correction See all GeneRIFs (17)
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables double-stranded RNA binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables enzyme binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables magnesium ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein homodimerization activity IPI
Inferred from Physical Interaction
more info
 PubMed 
enables ribonuclease III activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables ribonuclease III activity IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in RNA processing IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in RNA processing IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in mRNA processing IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in rRNA processing IEA
Inferred from Electronic Annotation
more info
  
involved_in rRNA processing IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in regulation of gene expression IBA
Inferred from Biological aspect of Ancestor
more info
  
Component Evidence Code Pubs
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
Preferred Names
RNase III
NP_417062.1

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    2703383..2704063 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_417062.1 RNase III [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_417062.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    D2K8Z1
    Conserved Domains (1) summary
    PRK00102
    Location:6 → 210
    rnc; ribonuclease III; Reviewed
Nucleotide Protein
Heading Accession and Version

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.