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iscU scaffold protein for [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 947002, updated on 4-Feb-2026
Official Symbol
iscU
Official Full Name
scaffold protein for
Primary source
ECOCYC:G7324
Locus tag
b2529
See related
ASAP:ABE-0008321
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK2526; nifU; yfhN
Summary
IscU fails to assemble Fe-S clusters in the presence of sodium citrate due to restricted free iron availability, however Fe-S cluster formation is restored, with sodium citrate present, in the presence of IscA. [More information is available at EcoGene: EG13395]. The iscSUA operon plays a role in assembly (or maintenance) of iron-sulfur clusters . [More information is available at EcoCyc: G7324].
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Sequence:
NC_000913.3 (2659903..2660289, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene Neighboring gene iron-sulfur cluster insertion protein IscA Neighboring gene cysteine desulfurase IscS Neighboring gene DNA-binding transcriptional dual regulator IscR

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Biosynthesis of iron-sulphur clusters is a complex and highly conserved process. Frazzon J, et al. Biochem Soc Trans, 2002 Aug. PMID 12196163
  2. Fe-S cluster assembly pathways in bacteria. Ayala-Castro C, et al. Microbiol Mol Biol Rev, 2008 Mar. PMID 18322036, Free PMC Article
  3. Iron-sulfur cluster biosynthesis. Bandyopadhyay S, et al. Biochem Soc Trans, 2008 Dec. PMID 19021507, Free PMC Article
  4. Bacterial IscU is a well folded and functional single domain protein. Adinolfi S, et al. Eur J Biochem, 2004 Jun. PMID 15153099
  5. The role of CyaY in iron sulfur cluster assembly on the E. coli IscU scaffold protein. Iannuzzi C, et al. PLoS One, 2011. PMID 21799759, Free PMC Article

See all (69) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Iron-sulfur cluster assembly scaffold protein IscU is required for activation of ferric uptake regulator (Fur) in Escherichiacoli.
    Title: Iron-sulfur cluster assembly scaffold protein IscU is required for activation of ferric uptake regulator (Fur) in Escherichiacoli.
  2. Evidence for dynamic in vivo interconversion of the conformational states of IscU during iron-sulfur cluster biosynthesis.
    Title: Evidence for dynamic in vivo interconversion of the conformational states of IscU during iron-sulfur cluster biosynthesis.
  3. We have demonstrated that a single mutation in the scaffold protein IscU rendered E. coli viability strictly dependent upon a functional CyaY.
    Title: Turning Escherichia coli into a Frataxin-Dependent Organism.
  4. Data indicate that the single point mutation IscU_D39A affects the 2Fe-2S coordination geometry.
    Title: Anatomy of an iron-sulfur cluster scaffold protein: Understanding the determinants of [2Fe-2S] cluster stability on IscU.
  5. Data indicate that IscU exists as two interconverting species: one (S) is largely structured with all four peptidyl-prolyl bonds trans; the other (D) contains an ordered domain that stabilizes two cis peptidyl-prolyl peptide bonds.
    Title: Metamorphic protein IscU alternates conformations in the course of its role as the scaffold protein for iron-sulfur cluster biosynthesis and delivery.
  6. The iron-sulfur cluster assembly in E. coli IscU is an IscS mediated biosynthetic process.
    Title: Biochemical characterization of iron-sulfur cluster assembly in the scaffold IscU of Escherichia coli.
  7. study determined the crystal structures of IscS-IscU and IscS-TusA complexes providing the first insight into their different modes of binding and the mechanism of sulfur transfer
    Title: Structural basis for Fe-S cluster assembly and tRNA thiolation mediated by IscS protein-protein interactions.
  8. Thioredoxin reductase system mediates iron binding in IscA and iron delivery for the iron-sulfur cluster assembly in IscU
    Title: Thioredoxin reductase system mediates iron binding in IscA and iron delivery for the iron-sulfur cluster assembly in IscU.
  9. Escherichia coli CYaY is an iron donor for the assembly of [2Fe-2S] clusters in the scaffold IscU
    Title: Iron-sulfur cluster biosynthesis: characterization of Escherichia coli CYaY as an iron donor for the assembly of [2Fe-2S] clusters in the scaffold IscU.
  10. both iron-sulfur cluster assembly protein IscU and co-chaperone HscB interact with the low peptide affinity T-state conformation of HscA ATPase and both modestly accelerate the rate-determining steps in the HscA reaction cycle
    Title: Regulation of the HscA ATPase reaction cycle by the co-chaperone HscB and the iron-sulfur cluster assembly protein IscU.
Submit: New GeneRIF Correction
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables 2 iron, 2 sulfur cluster binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables 2 iron, 2 sulfur cluster binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables 4 iron, 4 sulfur cluster binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables copper ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables ferrous iron binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables identical protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables iron ion binding IEA
Inferred from Electronic Annotation
more info
  
enables iron-sulfur cluster binding IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables zinc ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in L-cysteine catabolic process NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in intracellular iron ion homeostasis IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in iron-sulfur cluster assembly IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in iron-sulfur cluster assembly IEA
Inferred from Electronic Annotation
more info
  
involved_in iron-sulfur cluster assembly NAS
Non-traceable Author Statement
more info
 PubMed 
Component Evidence Code Pubs
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
Preferred Names
scaffold protein for

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    2659903..2660289 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_417024.1 scaffold protein for [Fe-S] cluster assembly

    See identical proteins and their annotated locations for NP_417024.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A1V2GF16, A0A5B9AR62
    Conserved Domains (1) summary
    PRK11325
    Location:1 → 127
    PRK11325; scaffold protein; Provisional
Nucleotide Protein
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