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der 50S ribosomal subunit stability factor [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 946983, updated on 26-Mar-2025
Official Symbol
der
Official Full Name
50S ribosomal subunit stability factor
Primary source
ECOCYC:G7319
Locus tag
b2511
See related
ASAP:ABE-0008270
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK2507; engA; yfgK
Summary
Der-depletion alters ribosome stability and/or biogenesis, as evidenced by polysome profiles (Bharat, 2006). [More information is available at EcoGene: EG14207]. The Der protein is associated with the large subunit of the ribosome and is required for its stability . [More information is available at EcoCyc: G7319].
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Sequence:
NC_000913.3 (2635884..2637356, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene inosine 5'-monophosphate dehydrogenase Neighboring gene exodeoxyribonuclease VII subunit XseA Neighboring gene zinc ribbon domain-containing protein YfgJ Neighboring gene outer membrane protein assembly factor BamB Neighboring gene ancillary SecYEG translocon subunit

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Structural insights into the function of a unique tandem GTPase EngA in bacterial ribosome assembly. Zhang X, et al. Nucleic Acids Res, 2014 Dec 1. PMID 25389271, Free PMC Article
  2. Phenotypic investigations of the depletion of EngA in Escherichia coli are consistent with a role in ribosome biogenesis. Bharat A, et al. FEMS Microbiol Lett, 2014 Apr. PMID 24822275, Free PMC Article
  3. An essential GTPase, der, containing double GTP-binding domains from Escherichia coli and Thermotoga maritima. Hwang J, et al. J Biol Chem, 2001 Aug 17. PMID 11387344
  4. Expression phenotypes suggest that Der participates in a specific, high affinity interaction with membranes. Lee R, et al. Protein Expr Purif, 2011 Jul. PMID 21354313
  5. Distinct GDP/GTP bound states of the tandem G-domains of EngA regulate ribosome binding. Tomar SK, et al. Nucleic Acids Res, 2009 Apr. PMID 19246542, Free PMC Article

See all (26) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. This analysis indicated that Der was present in sucrose gradient fractions containing membrane proteins. The interaction with the membrane fraction was specific for Der, since the related GTPase, Era, did not form the membrane complex.
    Title: Expression phenotypes suggest that Der participates in a specific, high affinity interaction with membranes.
  2. Der interacts with YihI, which activates the GTPase activity of Der.
    Title: A bacterial GAP-like protein, YihI, regulating the GTPase of Der, an essential GTP-binding protein in Escherichia coli.
  3. Data demonstrated that two GTPase domains of Der regulate its association with 50S subunit via the KH-like domain.
    Title: Interaction of an essential Escherichia coli GTPase, Der, with the 50S ribosome via the KH-like domain.
  4. Defective ribosome biogenesis due to mutations in two tandem GTP-binding domains of Der is suppressed by RelA overproduction.
    Title: RelA functionally suppresses the growth defect caused by a mutation in the G domain of the essential Der protein.
  5. Der plays a pivotal role in ribosome biogenesis possibly through interaction with rRNA or rRNA/r-protein complex.
    Title: The tandem GTPase, Der, is essential for the biogenesis of 50S ribosomal subunits in Escherichia coli.
Submit: New GeneRIF Correction
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables GTP binding IEA
Inferred from Electronic Annotation
more info
  
enables GTPase activating protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables GTPase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables guanosine tetraphosphate binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables nucleotide binding IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables ribosomal large subunit binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables ribosome binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables ribosome binding IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
acts_upstream_of_or_within ribosomal large subunit assembly IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in ribosome biogenesis IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
Preferred Names
50S ribosomal subunit stability factor
NP_417006.2

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    2635884..2637356 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_417006.2 50S ribosomal subunit stability factor [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_417006.2

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A5B9APX2
    Conserved Domains (1) summary
    PRK00093
    Location:3 → 462
    PRK00093; GTP-binding protein Der; Reviewed
Nucleotide Protein
Heading Accession and Version

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