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ppk polyphosphate kinase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 946971, updated on 25-Jun-2025
Official Symbol
ppk
Official Full Name
polyphosphate kinase
Primary source
ECOCYC:EG11510
Locus tag
b2501
See related
ASAP:ABE-0008235
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK2497
Summary
The role of polyphosphate (poly(Pi)) in Escherichia coli is still not fully understood; it may function in energy storage. [More information is available at EcoCyc: EG11510].
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Sequence:
NC_000913.3 (2623044..2625110)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene uracil phosphoribosyltransferase Neighboring gene phosphoribosylformylglycinamide cyclo-ligase Neighboring gene phosphoribosylglycinamide formyltransferase 1 Neighboring gene exopolyphosphatase Neighboring gene cyclic di-GMP phosphodiesterase PdeF

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. The multiple activities of polyphosphate kinase of Escherichia coli and their subunit structure determined by radiation target analysis. Tzeng CM, et al. J Biol Chem, 2000 Feb 11. PMID 10660553
  2. Catalytic properties of Escherichia coli polyphosphate kinase: an enzyme for ATP regeneration. Haeusler PA, et al. Biotechnol Appl Biochem, 1992 Apr. PMID 1316760
  3. The polyphosphate kinase gene of Escherichia coli. Isolation and sequence of the ppk gene and membrane location of the protein. Akiyama M, et al. J Biol Chem, 1992 Nov 5. PMID 1331061
  4. Genetically altered levels of inorganic polyphosphate in Escherichia coli. Crooke E, et al. J Biol Chem, 1994 Mar 4. PMID 8119977
  5. Phosphohistidyl active sites in polyphosphate kinase of Escherichia coli. Kumble KD, et al. Proc Natl Acad Sci U S A, 1996 Dec 10. PMID 8962061, Free PMC Article

See all (73) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Using Escherichia coli as a model organism, we have identified the first known mutations in polyP kinase that lead to increases in cellular polyP content.
    Title: Mutations in Escherichia coli Polyphosphate Kinase That Lead to Dramatically Increased In Vivo Polyphosphate Levels.
  2. These results indicate that ppk1 plays an important role in stress adaptation and virulence in meningitic E. coli K1 strain E44.
    Title: Polyphosphate kinase 1 is required for the pathogenesis process of meningitic Escherichia coli K1 (RS218).
  3. N-terminus verified by Edman degradation on mature peptide
    Title: The polyphosphate kinase gene of Escherichia coli. Isolation and sequence of the ppk gene and membrane location of the protein.
  4. Ppk is required for the increased rate of growth-dependent mutations produced by Pol IV overexpression; results suggest Ppk is affecting the activity of Pol IV in a manner that is independent of any previously known factors that regulate this polymerase
    Title: Polyphosphate kinase regulates error-prone replication by DNA polymerase IV in Escherichia coli.
  5. crystal structures of full-length Escherichia coli PPK and its complex with AMPPNP (beta-gamma-imidoadenosine 5-phosphate)is reported.
    Title: Crystal structure of a polyphosphate kinase and its implications for polyphosphate synthesis.
Submit: New GeneRIF Correction
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables diphosphotransferase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables phosphotransferase activity, phosphate group as acceptor IDA
Inferred from Direct Assay
more info
 PubMed 
enables polyphosphate kinase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables polyphosphate kinase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables polyphosphate:AMP phosphotransferase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein homodimerization activity IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in phosphorus metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in polyphosphate biosynthetic process IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within polyphosphate biosynthetic process IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within protein autophosphorylation IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within regulation of cell division IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in cytosol HDA PubMed 
is_active_in membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in periplasmic side of cell outer membrane IDA
Inferred from Direct Assay
more info
 PubMed 
part_of polyphosphate kinase complex IDA
Inferred from Direct Assay
more info
 PubMed 
Preferred Names
polyphosphate kinase
NP_416996.1

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    2623044..2625110
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_416996.1 polyphosphate kinase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_416996.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A376M4G5
    Conserved Domains (1) summary
    COG0855
    Location:2 → 688
    Ppk; Polyphosphate kinase [Inorganic ion transport and metabolism]
Nucleotide Protein
Heading Accession and Version

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