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cysS cysteine--tRNA ligase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 946969, updated on 30-Jul-2025
Official Symbol
cysS
Official Full Name
cysteine--tRNA ligase
Primary source
ECOCYC:EG10196
Locus tag
b0526
See related
ASAP:ABE-0001810
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK0519; syc
Summary
Cysteinyl-tRNA synthetase (CysRS) is a member of the family of aminoacyl-tRNA synthetases, which interpret the genetic code by covalently linking amino acids to their specific tRNA molecules. [More information is available at EcoCyc: EG10196].
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Sequence:
NC_000913.3 (554611..555996)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene UDP-2,3-diacylglucosamine diphosphatase Neighboring gene peptidyl-prolyl cis-trans isomerase B Neighboring gene PF04307 family inner membrane protein YbcI Neighboring gene putative RNA-binding protein YbcJ

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Cysteinyl-tRNA formation: the last puzzle of aminoacyl-tRNA synthesis. Li T, et al. FEBS Lett, 1999 Dec 3. PMID 10622715
  2. Cysteinyl-tRNA synthetase from Escherichia coli does not need an editing mechanism to reject serine and alanine. High binding energy of small groups in specific molecular interactions. Fersht AR, et al. Biochemistry, 1979 Apr 3. PMID 371674
  3. Temperature-sensitive mutants in cysteinyl-tRNA ligase of E. coli K-12. Bohman K, et al. Mol Gen Genet, 1979 Oct 2. PMID 396450
  4. The anticodon and discriminator base are major determinants of cysteine tRNA identity in vivo. Pallanck L, et al. J Biol Chem, 1992 Apr 15. PMID 1373131
  5. Sequence determination and modeling of structural motifs for the smallest monomeric aminoacyl-tRNA synthetase. Hou YM, et al. Proc Natl Acad Sci U S A, 1991 Feb 1. PMID 1992490, Free PMC Article

See all (32) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. N-terminus verified by Edman degradation on complete protein
    Title: Cysteinyl-tRNA synthetase: determination of the last E. coli aminoacyl-tRNA synthetase primary structure.
  2. cysteinyl-tRNA synthetase recognizes RNA in a shape specific manner
    Title: Shape-selective RNA recognition by cysteinyl-tRNA synthetase.
  3. Long-range covalent continuity between the active site domain at the N-terminus of cysteine tRNA synthetase and the cysteinyl-tRNA-binding anticodon domain at the enzyme C-terminus is required for efficient tRNA aminoacylaton.
    Title: Domain-domain communication for tRNA aminoacylation: the importance of covalent connectivity.
Submit: New GeneRIF Correction

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables ATP binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables ATP binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables aminoacyl-tRNA ligase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables aminoacyl-tRNA ligase activity IEA
Inferred from Electronic Annotation
more info
  
enables cysteine-tRNA ligase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables cysteine-tRNA ligase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables cysteine-tRNA ligase activity IEA
Inferred from Electronic Annotation
more info
  
enables ligase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables ligase activity IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables nucleotide binding IEA
Inferred from Electronic Annotation
more info
  
enables zinc ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables zinc ion binding IEA
Inferred from Electronic Annotation
more info
  
enables zinc ion binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in cysteinyl-tRNA aminoacylation IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within cysteinyl-tRNA aminoacylation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in cysteinyl-tRNA aminoacylation IEA
Inferred from Electronic Annotation
more info
  
involved_in tRNA aminoacylation for protein translation IEA
Inferred from Electronic Annotation
more info
  
involved_in translation IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
Preferred Names
cysteine--tRNA ligase
NP_415059.1

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    554611..555996
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_415059.1 cysteine--tRNA ligase

    See identical proteins and their annotated locations for NP_415059.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A2W6Q295
    Conserved Domains (1) summary
    PRK00260
    Location:1 → 461
    cysS; cysteinyl-tRNA synthetase; Validated
Nucleotide Protein
Heading Accession and Version

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