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hyfI hydrogenase 4 catalytic subunit HyfI [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 946966, updated on 23-Apr-2026
Official Symbol
hyfI
Official Full Name
hydrogenase 4 catalytic subunit HyfI
Primary source
ECOCYC:G7306
Locus tag
b2489
See related
ASAP:ABE-0008198
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK2485
Summary
This is the smaller of the two catalytic subunits. [More information is available at EcoCyc: G7306].
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Sequence:
NC_000913.3 (2610706..2611464)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene hydrogenase 4 component E Neighboring gene hydrogenase 4 component F Neighboring gene hydrogenase 4 catalytic subunit HyfG Neighboring gene hydrogenase 4 component H Neighboring gene putative hydrogenase 4 assembly protein Neighboring gene DNA-binding transcriptional activator HyfR Neighboring gene formate channel FocB

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Participation of hyf-encoded hydrogenase 4 in molecular hydrogen release coupled with proton-potassium exchange in Escherichia coli. Bagramyan K, et al. Membr Cell Biol, 2001. PMID 11817571
  2. Phosphorylation of ribosomal proteins in HeLa cells infected with vaccinia virus. Kaerlein M, et al. Nature, 1976 Jan 15. PMID 1246353
  3. Expression and regulation of a silent operon, hyf, coding for hydrogenase 4 isoenzyme in Escherichia coli. Self WT, et al. J Bacteriol, 2004 Jan. PMID 14702328, Free PMC Article
  4. Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features. Yamamoto Y, et al. DNA Res, 1997 Apr 28. PMID 9205837
  5. A 12-cistron Escherichia coli operon (hyf) encoding a putative proton-translocating formate hydrogenlyase system. Andrews SC, et al. Microbiology (Reading), 1997 Nov. PMID 9387241

See all (8) citations in PubMed

GeneRIFs: Gene References Into Functions

Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables 4 iron, 4 sulfur cluster binding IEA
Inferred from Electronic Annotation
more info
  
enables NADH dehydrogenase (ubiquinone) activity IEA
Inferred from Electronic Annotation
more info
  
enables iron-sulfur cluster binding IEA
Inferred from Electronic Annotation
more info
  
enables quinone binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in anaerobic electron transport chain IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in formate oxidation IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in proton transmembrane transport IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
part_of formate dehydrogenase complex IBA
Inferred from Biological aspect of Ancestor
more info
  
Preferred Names
hydrogenase 4 catalytic subunit HyfI

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    2610706..2611464
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_416984.1 hydrogenase 4 catalytic subunit HyfI

    See identical proteins and their annotated locations for NP_416984.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A6C9QC68, A0A8T5ZBK0
    Conserved Domains (1) summary
    COG3260
    Location:26 → 173
    HycG; Ni,Fe-hydrogenase III small subunit [Energy production and conversion]
Nucleotide Protein
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