U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

hyfF hydrogenase 4 component F [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 946962, updated on 4-Feb-2026
Official Symbol
hyfF
Official Full Name
hydrogenase 4 component F
Primary source
ECOCYC:G7303
Locus tag
b2486
See related
ASAP:ABE-0008191
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK2482
Summary
On the basis of sequence similarity to hycBCDEFG, which encodes hydrogenase 3, the ten-gene cluster hyfABCDEFGHIJ was presumed to encode a hydrogenase that interacts with formate dehydrogenase (FdhF) to produce an active formate hydrogenlyase complex. [More information is available at EcoCyc: G7303].
Try the new Gene page
Try the new Transcripts and proteins table
Sequence:
NC_000913.3 (2606917..2608497)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene hydrogenase 4 component D Neighboring gene hydrogenase 4 component E Neighboring gene hydrogenase 4 catalytic subunit HyfG Neighboring gene hydrogenase 4 component H Neighboring gene hydrogenase 4 catalytic subunit HyfI Neighboring gene putative hydrogenase 4 assembly protein

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Transcriptional control of hydrogen production during mixed carbon fermentation by hydrogenases 4 (hyf) and 3 (hyc) in Escherichia coli. Trchounian K. Gene, 2012 Sep 10. PMID 22771922
  2. Participation of hyf-encoded hydrogenase 4 in molecular hydrogen release coupled with proton-potassium exchange in Escherichia coli. Bagramyan K, et al. Membr Cell Biol, 2001. PMID 11817571
  3. Phosphorylation of ribosomal proteins in HeLa cells infected with vaccinia virus. Kaerlein M, et al. Nature, 1976 Jan 15. PMID 1246353
  4. Expression and regulation of a silent operon, hyf, coding for hydrogenase 4 isoenzyme in Escherichia coli. Self WT, et al. J Bacteriol, 2004 Jan. PMID 14702328, Free PMC Article
  5. A 12-cistron Escherichia coli operon (hyf) encoding a putative proton-translocating formate hydrogenlyase system. Andrews SC, et al. Microbiology (Reading), 1997 Nov. PMID 9387241

See all (10) citations in PubMed

GeneRIFs: Gene References Into Functions

Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables oxidoreductase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in DNA damage response IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within DNA damage response IEP
Inferred from Expression Pattern
more info
 PubMed 
involved_in anaerobic electron transport chain NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in anaerobic respiration NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in formate oxidation NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in glucose catabolic process NAS
Non-traceable Author Statement
more info
 PubMed 
Component Evidence Code Pubs
part_of [Ni-Fe] hydrogenase complex RCA
inferred from Reviewed Computational Analysis
more info
 PubMed 
located_in membrane NAS
Non-traceable Author Statement
more info
 PubMed 
located_in plasma membrane IEA
Inferred from Electronic Annotation
more info
  
located_in plasma membrane ISM
Inferred from Sequence Model
more info
 PubMed 
Preferred Names
hydrogenase 4 component F

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    2606917..2608497
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_416981.1 hydrogenase 4 component F

    See identical proteins and their annotated locations for NP_416981.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A7T2J2U5
    Conserved Domains (1) summary
    PRK06458
    Location:1 → 495
    PRK06458; hydrogenase 4 subunit F; Validated
Nucleotide Protein
Heading Accession and Version

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.