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nadE NH3-dependent NAD(+) synthetase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 946946, updated on 26-Mar-2025

Summary

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Official Symbol
nadE
Official Full Name
NH3-dependent NAD(+) synthetase
Primary source
ECOCYC:EG10663
Locus tag
b1740
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK1738; efg; ntrL
Summary
NadE is regulated by cyaR sRNA (De Lay, 2009). [More information is available at EcoGene: EG10663]. NAD synthetase is an essential enzyme involved in both the de novo biosynthesis and salvage of NAD+, catalyzing the final step of both pathways. [More information is available at EcoCyc: EG10663].
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Genomic context

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Sequence:
NC_000913.3 (1822458..1823285)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene N,N'-diacetylchitobiose-specific PTS enzyme IIB component Neighboring gene osmotically-inducible lipoprotein OsmE Neighboring gene excinuclease Cho Neighboring gene HutD family protein Ves Neighboring gene ATP-independent periplasmic chaperone

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Determining the extremes of the cellular NAD(H) level by using an Escherichia coli NAD(+)-auxotrophic mutant. Zhou Y, et al. Appl Environ Microbiol, 2011 Sep. PMID 21742902, Free PMC Article
  2. An essential gene (efg) located at 38.1 minutes on the Escherichia coli chromosome. Willison JC. J Bacteriol, 1992 Sep. PMID 1512214, Free PMC Article
  3. Location of an ntr-like gene on the physical map of Escherichia coli. Kim S, et al. J Bacteriol, 1990 Dec. PMID 2254242, Free PMC Article
  4. Complementation of nitrogen-regulatory (ntr-like) mutations in Rhodobacter capsulatus by an Escherichia coli gene: cloning and sequencing of the gene and characterization of the gene product. Allibert P, et al. J Bacteriol, 1987 Jan. PMID 3025172, Free PMC Article
  5. Biosynthesis of diphosphopyridine nucleotide. The purification and the properties of diphospyridine nucleotide synthetase from Escherichia coli b. Spencer RL, et al. J Biol Chem, 1967 Feb 10. PMID 4290215

See all (23) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. N-terminus verified by Edman degradation on complete protein
    Title: Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12.
  2. x-ray crystallographic study of Escherichia coli NAD synthetase
    Title: Structures of Escherichia coli NAD synthetase with substrates and products reveal mechanistic rearrangements.
Submit: New GeneRIF Correction

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables NAD+ synthase (glutamine-hydrolyzing) activity IEA
Inferred from Electronic Annotation
more info
  
enables NAD+ synthase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables NAD+ synthase activity IEA
Inferred from Electronic Annotation
more info
  
enables glutaminase activity IEA
Inferred from Electronic Annotation
more info
  
enables ligase activity IEA
Inferred from Electronic Annotation
more info
  
enables magnesium ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables nucleotide binding IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein homodimerization activity IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
acts_upstream_of_or_within 'de novo' NAD biosynthetic process from L-aspartate IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within 'de novo' NAD biosynthetic process from L-aspartate IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within DNA damage response IEP
Inferred from Expression Pattern
more info
 PubMed 
involved_in NAD biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within NAD biosynthetic process via the salvage pathway IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within NAD biosynthetic process via the salvage pathway IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
NH3-dependent NAD(+) synthetase
NP_416254.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    1822458..1823285
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_416254.1 NH3-dependent NAD(+) synthetase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_416254.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A8T5ZU89
    Conserved Domains (1) summary
    PRK00768
    Location:2 → 270
    nadE; ammonia-dependent NAD(+) synthetase
Nucleotide Protein
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