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amiA N-acetylmuramoyl-L-alanine amidase A [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 946916, updated on 30-Jul-2025
Official Symbol
amiA
Official Full Name
N-acetylmuramoyl-L-alanine amidase A
Primary source
ECOCYC:EG11823
Locus tag
b2435
See related
ASAP:ABE-0008032
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK2430; yfeE
Summary
Verified Tat substrate: [More information is available at EcoGene: EG11823]. Escherichia coli contains three N-acetylmuramyl-L-alanine amidases named AmiA, B, and C involved in cell division by splitting the murein septum. [More information is available at EcoCyc: EG11823].
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Sequence:
NC_000913.3 (2552352..2553221)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene DUF1131 domain-containing lipoprotein YfeY Neighboring gene putative inner membrane protein Neighboring gene putative acetyltransferase YpeA Neighboring gene coproporphyrinogen III oxidase Neighboring gene putative DNA-binding transcriptional regulator EutR

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Studying a cell division amidase using defined peptidoglycan substrates. Lupoli TJ, et al. J Am Chem Soc, 2009 Dec 30. PMID 19957935, Free PMC Article
  2. Rapid beta-lactam-induced lysis requires successful assembly of the cell division machinery. Chung HS, et al. Proc Natl Acad Sci U S A, 2009 Dec 22. PMID 19995973, Free PMC Article
  3. Envelope-bound N-acetylmuramyl-L-alanine amidase of Escherichia coli K 12. Purification and properties of the enzyme. van Heijenoort J, et al. Eur J Biochem, 1975 Oct 15. PMID 1102308
  4. Systematic characterization of curved DNA segments randomly cloned from Escherichia coli and their functional significance. Tanaka K, et al. Mol Gen Genet, 1991 May. PMID 1903834
  5. Modulation of Escherichia coli N-acetylmuramoyl-L-alanine amidase activity by phosphatidylglycerol. Vanderwinkel E, et al. Biochim Biophys Acta, 1985 Jan 28. PMID 2857097

See all (27) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. AmiA is a zinc metalloprotease that requires at least a tetrasaccharide glycopeptide substrate for cleavage.
    Title: Studying a cell division amidase using defined peptidoglycan substrates.
  2. Periplasmic amidases AmiC and AmiA, secreted by the twin arginine transport (Tat) pathway, are localized to the cytoplasm in Escherichia coli YqjA and YghB double mutant cells.
    Title: Inefficient Tat-dependent export of periplasmic amidases in an Escherichia coli strain with mutations in two DedA family genes.
Submit: New GeneRIF Correction

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables N-acetylmuramoyl-L-alanine amidase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables N-acetylmuramoyl-L-alanine amidase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables N-acetylmuramoyl-L-alanine amidase activity IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables zinc ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables zinc ion binding RCA
inferred from Reviewed Computational Analysis
more info
 PubMed 
Process Evidence Code Pubs
involved_in FtsZ-dependent cytokinesis IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within cell division EXP
Inferred from Experiment
more info
 PubMed 
involved_in cell wall organization IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within cellular response to antibiotic EXP
Inferred from Experiment
more info
 PubMed 
involved_in peptidoglycan catabolic process IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
is_active_in outer membrane-bounded periplasmic space IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in outer membrane-bounded periplasmic space IDA
Inferred from Direct Assay
more info
 PubMed 
located_in outer membrane-bounded periplasmic space ISM
Inferred from Sequence Model
more info
 PubMed 
located_in periplasmic space IDA
Inferred from Direct Assay
more info
 PubMed 
located_in periplasmic space IEA
Inferred from Electronic Annotation
more info
  
Preferred Names
N-acetylmuramoyl-L-alanine amidase A
NP_416930.1

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    2552352..2553221
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_416930.1 N-acetylmuramoyl-L-alanine amidase A

    See identical proteins and their annotated locations for NP_416930.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A8T5Y666
    Conserved Domains (1) summary
    PRK10319
    Location:1 → 287
    PRK10319; N-acetylmuramoyl-L-alanine amidase AmiA
Nucleotide Protein
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