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pdxH pyridoxine/pyridoxamine 5'-phosphate oxidase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 946806, updated on 26-Mar-2025

Summary

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Official Symbol
pdxH
Official Full Name
pyridoxine/pyridoxamine 5'-phosphate oxidase
Primary source
ECOCYC:EG11487
Locus tag
b1638
See related
ASAP:ABE-0005482
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK1634
Summary
The pdxH gene codes for a monofunctional flavoprotein enzyme that can accept either pyridoxine 5'-phosphate (PNP) or pyridoxamine 5'-phosphate (PMP) as substrates. [More information is available at EcoCyc: EG11487].
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Genomic context

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Sequence:
NC_000913.3 (1717351..1718007, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene pyridoxal kinase 2 Neighboring gene tyrosine--tRNA ligase Neighboring gene inhibitor of c-type lysozyme, putative lipoprotein Neighboring gene anhydro-N-acetylmuramic acid kinase

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Lysine acetylation regulates the activity of Escherichia coli pyridoxine 5'-phosphate oxidase. Gu J, et al. Acta Biochim Biophys Sin (Shanghai), 2017 Feb 6. PMID 28039149
  2. Identification and characterization of the pyridoxal 5'-phosphate allosteric site in Escherichia coli pyridoxine 5'-phosphate oxidase. Barile A, et al. J Biol Chem, 2021 Jan-Jun. PMID 34019876, Free PMC Article
  3. Genetic map position of the pdxH gene in Escherichia coli. Shimizu S, et al. J Bacteriol, 1976 Sep. PMID 783152, Free PMC Article
  4. Purification and characterization of the pyridoxol-5'-phosphate:oxygen oxidoreductase (deaminating) from Escherichia coli. Notheis C, et al. Biochim Biophys Acta, 1995 Mar 15. PMID 7696318
  5. Synthesis of Pyridoxine by a Pyridoxal Auxotroph of Escherichia coli. Dempsey WB. J Bacteriol, 1966 Aug. PMID 16562115, Free PMC Article

See all (26) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Site-directed mutagenesis analysis indicated that acetylated lysine residues play important roles in the enzymatic activity and enzymatic properties of pyridoxine 5'-phosphate oxidase (EcPNPOx).
    Title: Lysine acetylation regulates the activity of Escherichia coli pyridoxine 5'-phosphate oxidase.
  2. N-terminus verified by Edman degradation on mature peptide
    Title: Kinetic limitation and cellular amount of pyridoxine (pyridoxamine) 5'-phosphate oxidase of Escherichia coli K-12.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables FMN binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables FMN binding IEA
Inferred from Electronic Annotation
more info
  
enables oxidoreductase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables oxidoreductase activity IEA
Inferred from Electronic Annotation
more info
  
enables oxidoreductase activity, acting on the CH-NH2 group of donors IEA
Inferred from Electronic Annotation
more info
  
enables phosphate ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein homodimerization activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables pyridoxal phosphate binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables pyridoxamine phosphate oxidase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables pyridoxamine phosphate oxidase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables pyridoxamine phosphate oxidase activity IEA
Inferred from Electronic Annotation
more info
  
enables riboflavin binding IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in 'de novo' pyridoxal 5'-phosphate biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in pyridoxal phosphate biosynthetic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in pyridoxine biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in vitamin B6 metabolic process IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
part_of protein-containing complex IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
pyridoxine/pyridoxamine 5'-phosphate oxidase
NP_416155.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    1717351..1718007 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_416155.1 pyridoxine/pyridoxamine 5'-phosphate oxidase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_416155.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A5B9AIY8, A0A8S7EG98
    Conserved Domains (1) summary
    PRK05679
    Location:24 → 218
    PRK05679; pyridoxal 5'-phosphate synthase
Nucleotide Protein
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