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nuoE NADH:quinone oxidoreductase subunit E [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 946746, updated on 4-Feb-2026
Official Symbol
nuoE
Official Full Name
NADH:quinone oxidoreductase subunit E
Primary source
ECOCYC:EG12086
Locus tag
b2285
See related
ASAP:ABE-0007547
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK2279
Summary
NuoE is part of the soluble fragment of NADH dehydrogenase I, which represents the electron input part of the enzyme . [More information is available at EcoCyc: EG12086].
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Sequence:
NC_000913.3 (2401552..2402052, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene NADH:quinone oxidoreductase subunit I Neighboring gene NADH:quinone oxidoreductase subunit H Neighboring gene NADH:quinone oxidoreductase subunit G Neighboring gene NADH:quinone oxidoreductase subunit F Neighboring gene NADH:quinone oxidoreductase subunit CD Neighboring gene NADH:quinone oxidoreductase subunit B

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Significance of [2Fe-2S] Cluster N1a for Electron Transfer and Assembly of Escherichia coli Respiratory Complex I. Dörner K, et al. Biochemistry, 2017 Jun 6. PMID 28509551
  2. Amino acid residues associated with cluster N3 in the NuoF subunit of the proton-translocating NADH-quinone oxidoreductase from Escherichia coli. Velazquez I, et al. FEBS Lett, 2005 Jun 6. PMID 15922336
  3. Spin labeling of the Escherichia coli NADH ubiquinone oxidoreductase (complex I). Pohl T, et al. Biochim Biophys Acta, 2010 Dec. PMID 20959113
  4. Adenosine diphosphate sugar pyrophosphatase prevents glycogen biosynthesis in Escherichia coli. Moreno-Bruna B, et al. Proc Natl Acad Sci U S A, 2001 Jul 3. PMID 11416161, Free PMC Article
  5. Metabolic regulation analysis of icd-gene knockout Escherichia coli based on 2D electrophoresis with MALDI-TOF mass spectrometry and enzyme activity measurements. Kabir MM, et al. Appl Microbiol Biotechnol, 2004 Jul. PMID 15221231

See all (65) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Study determined the distance between a MTSL labeled complex I variant and the bound quinone by continuous-wave (cw) EPR allowing an inference on the location of the quinone binding site.
    Title: Spin labeling of the Escherichia coli NADH ubiquinone oxidoreductase (complex I).
  2. N-terminus verified by Edman degradation on complete protein
    Title: Adenosine diphosphate sugar pyrophosphatase prevents glycogen biosynthesis in Escherichia coli.
Submit: New GeneRIF Correction
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables 2 iron, 2 sulfur cluster binding IEA
Inferred from Electronic Annotation
more info
  
enables 2 iron, 2 sulfur cluster binding IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables 2 iron, 2 sulfur cluster binding ISS
Inferred from Sequence or Structural Similarity
more info
 PubMed 
contributes_to FMN binding IDA
Inferred from Direct Assay
more info
 PubMed 
contributes_to NADH dehydrogenase (ubiquinone) activity IDA
Inferred from Direct Assay
more info
 PubMed 
contributes_to NADH dehydrogenase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
contributes_to NADH dehydrogenase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables iron-sulfur cluster binding IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables oxidoreductase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables quinone binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in proton transmembrane transport IEA
Inferred from Electronic Annotation
more info
  
involved_in respiratory electron transport chain IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in respiratory electron transport chain IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
part_of NADH dehydrogenase complex IDA
Inferred from Direct Assay
more info
 PubMed 
located_in membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in membrane IMP
Inferred from Mutant Phenotype
more info
 PubMed 
located_in plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
Preferred Names
NADH:quinone oxidoreductase subunit E
NP_416788.1

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    2401552..2402052 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_416788.1 NADH:quinone oxidoreductase subunit E

    See identical proteins and their annotated locations for NP_416788.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A5B9AI56, A0A6C9QG37
    Conserved Domains (1) summary
    PRK07539
    Location:14 → 166
    PRK07539; NADH-quinone oxidoreductase subunit NuoE
Nucleotide Protein
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