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menF isochorismate synthase MenF [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 946712, updated on 30-Jul-2025
Official Symbol
menF
Official Full Name
isochorismate synthase MenF
Primary source
ECOCYC:EG12362
Locus tag
b2265
See related
ASAP:ABE-0007485
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK2259; yfbA
Summary
There are two isochorismate synthase enzymes present in Escherichia coli, encoded by menF and entC. [More information is available at EcoCyc: EG12362].
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Sequence:
NC_000913.3 (2379348..2380643, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene o-succinylbenzoate synthase Neighboring gene 1,4-dihydroxy-2-naphthoyl-CoA synthase Neighboring gene 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase Neighboring gene 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase Neighboring gene tail-anchored inner membrane protein ElaB Neighboring gene putative acetyltransferase ElaA

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Structure of isochorismate synthase in complex with magnesium. Parsons JF, et al. Acta Crystallogr D Biol Crystallogr, 2008 May. PMID 18453696, Free PMC Article
  2. Lysine 190 is the catalytic base in MenF, the menaquinone-specific isochorismate synthase from Escherichia coli: implications for an enzyme family. Kolappan S, et al. Biochemistry, 2007 Jan 30. PMID 17240978
  3. Clustering of isochorismate synthase genes menF and entC and channeling of isochorismate in Escherichia coli. Buss K, et al. Biochim Biophys Acta, 2001 Dec 30. PMID 11779629
  4. Menaquinone (vitamin K2) biosynthesis: overexpression, purification, and characterization of a new isochorismate synthase from Escherichia coli. Daruwala R, et al. J Bacteriol, 1997 May. PMID 9150206, Free PMC Article
  5. A new isochorismate synthase specifically involved in menaquinone (vitamin K2) biosynthesis encoded by the menF gene. Daruwala R, et al. FEMS Microbiol Lett, 1996 Jul 1. PMID 8764478

See all (16) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Lys190 has been identified as the base that activates water for nucleophilic attack at the chorismate C2 carbon.
    Title: Lysine 190 is the catalytic base in MenF, the menaquinone-specific isochorismate synthase from Escherichia coli: implications for an enzyme family.
Submit: New GeneRIF Correction
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables isochorismate synthase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables isochorismate synthase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables isochorismate synthase activity IEA
Inferred from Electronic Annotation
more info
  
enables isomerase activity IEA
Inferred from Electronic Annotation
more info
  
enables magnesium ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables magnesium ion binding IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in menaquinone biosynthetic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in menaquinone biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within menaquinone biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Preferred Names
isochorismate synthase MenF
NP_416768.4

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    2379348..2380643 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_416768.4 isochorismate synthase MenF

    See identical proteins and their annotated locations for NP_416768.4

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A376FZE5
    Conserved Domains (1) summary
    PRK15012
    Location:1 → 431
    PRK15012; menaquinone-specific isochorismate synthase; Provisional
Nucleotide Protein
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