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ada DNA-binding transcriptional dual regulator/DNA repair protein Ada [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 946710, updated on 23-Apr-2026
Official Symbol
ada
Official Full Name
DNA-binding transcriptional dual regulator/DNA repair protein Ada
Primary source
ECOCYC:EG10029
Locus tag
b2213
See related
ASAP:ABE-0007311
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK2205
Summary
The Ada N-terminal domain acts as a transcriptional activator of ada, alkA, alkB and aidB. [More information is available at EcoGene: EG10029]. O6-methylguanine and O4-methylthymine are the major mutagenic lesions resulting from exposure of DNA to simple alkylating agents such as N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), N-methyl-N-nitrosourea (MNU) and, to a lesser degree, methane methanesulfonate (MMS). [More information is available at EcoCyc: EG10029].
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Sequence:
NC_000913.3 (2309341..2310405, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene ABC transporter family protein/microcin J25 efflux protein Neighboring gene DNA oxidative demethylase Neighboring gene FAD:protein FMN transferase Neighboring gene outer membrane porin C

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Molecular cloning of a gene which regulates the adaptive response to alkylating agents in Escherichia coli. Sedgwick B. Mol Gen Genet, 1983. PMID 6355769
  2. Reversible folding of Ada protein (O6-methylguanine-DNA methyltransferase) of Escherichia coli. Bhattacharyya D, et al. Biochemistry, 1998 Feb 10. PMID 9484244
  3. Metal-coordination sphere in the methylated Ada protein-DNA co-complex. Myers LC, et al. Chem Biol, 1994 Oct. PMID 9383376
  4. Specificity of DNA repair methyltransferases determined by competitive inactivation with oligonucleotide substrates: evidence that Escherichia coli Ada repairs O6-methylguanine and O4-methylthymine with similar efficiency. Paalman SR, et al. Biochemistry, 1997 Sep 16. PMID 9287154
  5. Sequence-specific DNA recognition of the Escherichia coli Ada protein associated with the methylation-dependent functional switch for transcriptional regulation. Sakashita H, et al. J Biochem, 1995 Dec. PMID 8720133

See all (79) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. spatial tracking measurements of C-terminal domain of Ada moving on DNA extended by flow; study concludes sliding is mechanism of C-Ada motion along stretched DNA; suggests C-Ada could conduct repair while moving at near the 1-dimensional diffusion limit
    Title: Using the bias from flow to elucidate single DNA repair protein sliding and interactions with DNA.
  2. N-terminus verified by Edman degradation on complete protein
    Title: Purification and structure of the intact Ada regulatory protein of Escherichia coli K12, O6-methylguanine-DNA methyltransferase.
  3. Molecular modeling of the N-terminal 16-kDa domain of Ada protein indicates that the methyl acceptor residue is Cys38.
    Title: The solution structure of the methylated form of the N-terminal 16-kDa domain of Escherichia coli Ada protein.
  4. NMR assignments of the N-terminal 16 kDa domain of Escherichia coli Ada protein
    Title: 1H, 13C and 15N resonance assignments of the N-terminal 16 kDa domain of Escherichia coli Ada protein.
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Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables DNA-binding transcription factor activity IEA
Inferred from Electronic Annotation
more info
  
enables DNA-binding transcription factor activity TAS
Traceable Author Statement
more info
 PubMed 
enables catalytic activity IEA
Inferred from Electronic Annotation
more info
  
enables methylated-DNA-[protein]-cysteine S-methyltransferase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables methylated-DNA-[protein]-cysteine S-methyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables methyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables sequence-specific DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables zinc ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables zinc ion binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in DNA alkylation repair EXP
Inferred from Experiment
more info
 PubMed 
involved_in DNA alkylation repair IEA
Inferred from Electronic Annotation
more info
  
involved_in DNA damage response IEP
Inferred from Expression Pattern
more info
 PubMed 
involved_in DNA repair IEA
Inferred from Electronic Annotation
more info
  
involved_in negative regulation of DNA-templated transcription IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in positive regulation of DNA-templated transcription IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in regulation of DNA-templated transcription IEA
Inferred from Electronic Annotation
more info
  
Preferred Names
DNA-binding transcriptional dual regulator/DNA repair protein Ada
NP_416717.1

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    2309341..2310405 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_416717.1 DNA-binding transcriptional dual regulator/DNA repair protein Ada

    See identical proteins and their annotated locations for NP_416717.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A776QQU1, A0A8S7RC27
    Conserved Domains (1) summary
    PRK15435
    Location:1 → 353
    PRK15435; bifunctional DNA-binding transcriptional regulator/O6-methylguanine-DNA methyltransferase Ada
Nucleotide Protein
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