gyrA [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Official Symbol: gyrA
Primary source: ECOCYC:EG10423
Locus tag: b2231
See related: ASAP:ABE-0007370
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: ECK2223; hisW; nalA; nfxA; norA; parD
Summary: gyrA is an essential gene. [More information is available at EcoGene: EG10423]. DNA gyrase is one of two essential members of the type II topoisomerase family in Escherichia coli. [More information is available at EcoCyc: EG10423].
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Genomic context

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Sequence:: NC_000913.3 (2336793..2339420, complement)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Single-molecule dynamics of DNA gyrase in evolutionarily distant bacteria Mycobacterium tuberculosis and Escherichia coli.
Title: Single-molecule dynamics of DNA gyrase in evolutionarily distant bacteria Mycobacterium tuberculosis and Escherichia coli.
Escherichia coli GyrA Tower Domain Interacts with QnrB1 Loop B and Plays an Important Role in QnrB1 Protection from Quinolone Inhibition.
Title: Escherichia coli GyrA Tower Domain Interacts with QnrB1 Loop B and Plays an Important Role in QnrB1 Protection from Quinolone Inhibition.
Our findings indicate that the catalytic cycle of E. coli gyrase operates at high thermodynamic efficiency, and that the stability of DNA wrapping by the CTD provides one limit to DNA supercoil introduction, beyond which strand passage competes with ATP-dependent supercoil relaxation
Title: Modulated control of DNA supercoiling balance by the DNA-wrapping domain of bacterial gyrase.
Inhibition of the gyrA promoter by transcription-coupled DNA supercoiling in Escherichia coli has been demonstrated.
Title: Inhibition of the gyrA promoter by transcription-coupled DNA supercoiling in Escherichia coli.
The gyrA mutations were identified in nucleotide positions 248, 255, 259, 260, 261, 273 and 300, and mutations at positions 248 and 259 resulting in amino acid changes at positions 83 and 87 were associated with quinolone resistance.
Title: CRISPR/Cas9/sgRNA-mediated targeted gene modification confirms the cause-effect relationship between gyrA mutation and quinolone resistance in Escherichia coli.
High frequency of aac(6')-Ib-cr gene associated with double mutations in gyrA and parC is related to fluoroquinolone resistance
Title: High frequency of aac(6')-Ib-cr gene associated with double mutations in gyrA and parC in Escherichia coli isolates from patients with urinary tract infections.
Measuring the dwell time of gyrase bound to DNA revealed that most gyrase remain immobile for approximately 2 s, whereas enzymes in the vicinity of the replisome had a approximately 8 s dwell time, suggesting that when an excessive (+) supercoiling is present due to the fast progression of the fork, gyrase performs multiple rounds of catalysis without dissociating from DNA.
Title: Single-molecule imaging of DNA gyrase activity in living Escherichia coli.
analysis of gyrase mutations in E.coli that could be responsible for increased quinolone resistive mechanisms among enteric pathogens; docking studies revealed displacement of quinolone binding site in mutated protein complex which resulted in lower binding energy as compared to the normal one
Title: Analytical profiling of mutations in quinolone resistance determining region of gyrA gene among UPEC.
The present study was undertaken to better understand the dynamic behavior of the gyrA in Enterotoxigenic Escherichia coli [ETEC] and to decipher the structural changes associated with mutations, Ser83Leu and Ser83Leu/Asp87Asn, leading to ciprofloxacin antibiotic resistance in ETEC gyrA.
Title: Travelers' Diarrhea-Associated Enterotoxigenic Escherichia coli gyrA Mutants and Quinolone Antibiotic Affinity: A Molecular Dynamics Simulation and Residue Interaction Network Analysis.
Data indicate the existence of interactions between the fluoroquinolone C-7 ring and both GyrA and GyrB.
Title: Fluoroquinolone-gyrase-DNA complexes: two modes of drug binding.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by EcoCyc

Function	Evidence Code	Pubs
enables ATP binding	IBA Inferred from Biological aspect of Ancestor more info
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP-dependent activity, acting on DNA	IDA Inferred from Direct Assay more info	PubMed
enables DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA negative supercoiling activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA negative supercoiling activity	IEA Inferred from Electronic Annotation more info
enables DNA topoisomerase activity	IEA Inferred from Electronic Annotation more info
enables DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity	IEA Inferred from Electronic Annotation more info
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA topological change	IBA Inferred from Biological aspect of Ancestor more info
involved_in DNA topological change	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within DNA topological change	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in DNA-templated DNA replication	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within DNA-templated transcription	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within DNA-templated transcription	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in chromosome organization	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of DNA-templated DNA replication	IDA Inferred from Direct Assay more info	PubMed
involved_in response to antibiotic	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within response to xenobiotic stimulus	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex	IBA Inferred from Biological aspect of Ancestor more info
located_in chromosome	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	HDA more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	HDA more info	PubMed

General protein information

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Preferred Names: DNA gyrase subunit A

NP_416734.1

EC 5.6.2.2

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_000913.3 Reference assembly

Range

2336793..2339420 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NP_416734.1 DNA gyrase subunit A [Escherichia coli str. K-12 substr. MG1655]

See identical proteins and their annotated locations for NP_416734.1

Status: PROVISIONAL

UniProtKB/TrEMBL

Q47245

Conserved Domains (2) summary

PRK05560
Location:7 → 842

PRK05560; DNA gyrase subunit A; Validated

sd00041
Location:538 → 585

GyrA-ParC_C; GyrA_CTD repeat [structural motif]