sbcB exodeoxyribonuclease I [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Official Symbol: sbcB
Official Full Name: exodeoxyribonuclease I
Primary source: ECOCYC:EG10926
Locus tag: b2011
See related: ASAP:ABE-0006686
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: cpeA; ECK2005; exoI; rmuA; xonA
Summary: The C-terminus of Ssb binds and stimulates Exonuclease I activity (Lu, 2008). [More information is available at EcoGene: EG10926]. Exonuclease I (ExoI) hydrolyzes DNA through its 3'-5' exonuclease acitivity. [More information is available at EcoCyc: EG10926].
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Genomic context

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Sequence:: NC_000913.3 (2082756..2084183)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Data show that replication forks form a palindrome-like structural intermediate that requires nucleolytic processing by SbcC-SbcD and exonuclease I (ExoI) before chromosome replication can be completed.
Title: SbcC-SbcD and ExoI process convergent forks to complete chromosome replication.
ExoI is primarily responsible for more efficiently abort template-switches on the lagging strand and that its ability to be recruited by single-strand DNA binding protein plays a critical role in quasipalindrome-associated mutagenesis avoidance and strand bias.
Title: SSB recruitment of Exonuclease I aborts template-switching in Escherichia coli.
Possible mechanisms for ExoI processive digestion are proposed based upon crystal structures of ExoI in complex with four different single-stranded DNA substrates.
Title: Crystal structures of Escherichia coli exonuclease I in complex with single-stranded DNA provide insights into the mechanism of processive digestion.
Study hypothesized that RecJ and XonA blunt the double-strand end (created by the replication fork reversal)in order to produce SOS(Con) expression.
Title: RecA4142 causes SOS constitutive expression by loading onto reversed replication forks in Escherichia coli K-12.
The absence of SbcC and SbcD in sbcB15 cells decreased stationary-phase mutation.
Title: Role of single-strand DNA 3'-5' exonuclease ExoI and nuclease SbcCD in stationary-phase mutation in Escherichia coli K-12.
Data show structure of ExoI in complex with thymidine 5'-monophosphate, indicating that conserved active site contains a unique metal-binding position when compared with orthologous sites in the Klenow fragment, T4 DNA polymerase and dnaQ.
Title: Structure of Escherichia coli exonuclease I in complex with thymidine 5'-monophosphate.
N-terminus verified by Edman degradation on complete protein
Title: Determination of the nucleotide sequence for the exonuclease I structural gene (sbcB) of Escherichia coli K12.
Our results suggest that two types of sbcB-sensitive RecF pathways can be distinguished in E. coli, one that is activated by the sbcB15 mutation and one that is activated by sbcB null mutations.
Title: sbcB15 And DeltasbcB mutations activate two types of recf recombination pathways in Escherichia coli.

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General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by EcoCyc

Function	Evidence Code	Pubs
enables 3'-5'-RNA exonuclease activity	IEA Inferred from Electronic Annotation more info
enables 5'-deoxyribose-5-phosphate lyase activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA exonuclease activity	IEA Inferred from Electronic Annotation more info
enables exonuclease activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables magnesium ion binding	IDA Inferred from Direct Assay more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables nuclease activity	IEA Inferred from Electronic Annotation more info
enables nucleic acid binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables single-stranded DNA 3'-5' DNA exonuclease activity	IDA Inferred from Direct Assay more info	PubMed
enables single-stranded DNA 3'-5' DNA exonuclease activity	IEA Inferred from Electronic Annotation more info
enables single-stranded DNA binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within DNA catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in DNA catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in DNA damage response	IEA Inferred from Electronic Annotation more info
involved_in DNA metabolic process	IEA Inferred from Electronic Annotation more info
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within DNA replication termination	EXP Inferred from Experiment more info	PubMed