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hisF imidazole glycerol phosphate synthase subunit HisF [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 946516, updated on 4-Feb-2026
Official Symbol
hisF
Official Full Name
imidazole glycerol phosphate synthase subunit HisF
Primary source
ECOCYC:EG10448
Locus tag
b2025
See related
ASAP:ABE-0006727
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK2020
Summary
N-terminus of purified HisF was sequenced by Klem and Davisson (1993), and the fMet is not clipped. [More information is available at EcoGene: EG10448]. Imidazole glycerol phosphate synthase (HisFH) is a heterodimer that carries out the fifth step in histidine biosynthesis, as well as generating aminoimidazole carboxamide ribonucleotide, an intermediate in purine nucleotide biosynthesis. [More information is available at EcoCyc: EG10448].
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Sequence:
NC_000913.3 (2095844..2096620)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene histidinal/histidinol dehydrogenase Neighboring gene histidinol-phosphate aminotransferase Neighboring gene imidazoleglycerol-phosphate dehydratase/histidinol-phosphatase Neighboring gene imidazole glycerol phosphate synthase subunit HisH Neighboring gene 1-(5-phosphoribosyl)-5- Neighboring gene putative bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase Neighboring gene O-antigen co-polymerase Neighboring gene UDP-glucose 6-dehydrogenase

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Nanometer propagation of millisecond motions in V-type allostery. Lipchock JM, et al. Structure, 2010 Dec 8. PMID 21134639, Free PMC Article
  2. In vivo evaluation of the interaction between the Escherichia coli IGP synthase subunits using the Bacterial Two-Hybrid system. Chioccioli S, et al. FEMS Microbiol Lett, 2020 Jul 1. PMID 32614412
  3. Suppression of the pleiotropic effects of HisH and HisF overproduction identifies four novel loci on the Salmonella typhimurium chromosome: osmH, sfiW, sfiX, and sfiY. Flores A, et al. J Bacteriol, 1995 Sep. PMID 7665459, Free PMC Article
  4. Excess histidine enzymes cause AICAR-independent filamentation in Escherichia coli. Frandsen N, et al. Mol Gen Genet, 1993 Sep. PMID 8413183
  5. Imidazole glycerol phosphate synthase: the glutamine amidotransferase in histidine biosynthesis. Klem TJ, et al. Biochemistry, 1993 May 18. PMID 8494895

See all (25) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. In vivo evaluation of the interaction between the Escherichia coli IGP synthase subunits using the Bacterial Two-Hybrid system.
    Title: In vivo evaluation of the interaction between the Escherichia coli IGP synthase subunits using the Bacterial Two-Hybrid system.
  2. Enzyme activation in the ternary complex is primarily due to an enhancement of ms motions that allows formation of a population of enzymatically active conformers.
    Title: Nanometer propagation of millisecond motions in V-type allostery.
Submit: New GeneRIF Correction
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables imidazoleglycerol-phosphate synthase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables imidazoleglycerol-phosphate synthase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables imidazoleglycerol-phosphate synthase activity IEA
Inferred from Electronic Annotation
more info
  
enables lyase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
acts_upstream_of_or_within L-histidine biosynthetic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in L-histidine biosynthetic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in L-histidine biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in amino acid biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
part_of imidazoleglycerol-phosphate synthase complex IDA
Inferred from Direct Assay
more info
 PubMed 
Preferred Names
imidazole glycerol phosphate synthase subunit HisF
NP_416529.1

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    2095844..2096620
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_416529.1 imidazole glycerol phosphate synthase subunit HisF

    See identical proteins and their annotated locations for NP_416529.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A828P6I4
    Conserved Domains (1) summary
    PRK02083
    Location:1 → 257
    PRK02083; imidazole glycerol phosphate synthase subunit HisF; Provisional
Nucleotide Protein
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