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hisF imidazole glycerol phosphate synthase subunit HisF [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 946516, updated on 19-Sep-2024

Summary

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Official Symbol
hisF
Official Full Name
imidazole glycerol phosphate synthase subunit HisF
Primary source
ECOCYC:EG10448
Locus tag
b2025
See related
ASAP:ABE-0006727
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK2020
Summary
N-terminus of purified HisF was sequenced by Klem and Davisson (1993), and the fMet is not clipped. [More information is available at EcoGene: EG10448]. Imidazole glycerol phosphate synthase (HisFH) is a heterodimer that carries out the fifth step in histidine biosynthesis, as well as generating aminoimidazole carboxamide ribonucleotide, an intermediate in purine nucleotide biosynthesis. [More information is available at EcoCyc: EG10448].
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Genomic context

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Sequence:
NC_000913.3 (2095844..2096620)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene histidinal/histidinol dehydrogenase Neighboring gene histidinol-phosphate aminotransferase Neighboring gene imidazoleglycerol-phosphate dehydratase/histidinol-phosphatase Neighboring gene imidazole glycerol phosphate synthase subunit HisH Neighboring gene 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase Neighboring gene putative bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase Neighboring gene O-antigen co-polymerase Neighboring gene UDP-glucose 6-dehydrogenase

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Nanometer propagation of millisecond motions in V-type allostery. Lipchock JM, et al. Structure, 2010 Dec 8. PMID 21134639, Free PMC Article
  2. In vivo evaluation of the interaction between the Escherichia coli IGP synthase subunits using the Bacterial Two-Hybrid system. Chioccioli S, et al. FEMS Microbiol Lett, 2020 Jul 1. PMID 32614412
  3. Kinetic model of imidazologlycerol-phosphate synthetase from Escherichia coli. Demin OV, et al. Biochemistry (Mosc), 2004 Dec. PMID 15627387
  4. On the structure of hisH: protein structure prediction in the context of structural and functional genomics. O'Donoghue P, et al. J Struct Biol, 2001 May-Jun. PMID 11551184
  5. Imidazole glycerol phosphate synthase: the glutamine amidotransferase in histidine biosynthesis. Klem TJ, et al. Biochemistry, 1993 May 18. PMID 8494895

See all (26) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. In vivo evaluation of the interaction between the Escherichia coli IGP synthase subunits using the Bacterial Two-Hybrid system.
    Title: In vivo evaluation of the interaction between the Escherichia coli IGP synthase subunits using the Bacterial Two-Hybrid system.
  2. Enzyme activation in the ternary complex is primarily due to an enhancement of ms motions that allows formation of a population of enzymatically active conformers.
    Title: Nanometer propagation of millisecond motions in V-type allostery.
Submit: New GeneRIF Correction

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables imidazoleglycerol-phosphate synthase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables imidazoleglycerol-phosphate synthase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables imidazoleglycerol-phosphate synthase activity IEA
Inferred from Electronic Annotation
more info
  
enables lyase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
acts_upstream_of_or_within L-histidine biosynthetic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in L-histidine biosynthetic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in L-histidine biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
part_of imidazoleglycerol-phosphate synthase complex IDA
Inferred from Direct Assay
more info
 PubMed 
part_of imidazoleglycerol-phosphate synthase complex IPI
Inferred from Physical Interaction
more info
 PubMed 

General protein information

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Preferred Names
imidazole glycerol phosphate synthase subunit HisF
NP_416529.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    2095844..2096620
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_416529.1 imidazole glycerol phosphate synthase subunit HisF [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_416529.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A828P6I4
    Conserved Domains (1) summary
    PRK02083
    Location:1 → 257
    PRK02083; imidazole glycerol phosphate synthase subunit HisF; Provisional
Nucleotide Protein
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