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ruvB Holliday junction branch migration complex subunit RuvB [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 946371, updated on 26-Mar-2025
Official Symbol
ruvB
Official Full Name
Holliday junction branch migration complex subunit RuvB
Primary source
ECOCYC:RUVB
Locus tag
b1860
See related
ASAP:ABE-0006204
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK1861
Summary
May directly convert replication forks into Holliday junctions. [More information is available at EcoGene: EG10924]. RuvABC is an enzymatic complex which functions in recombination pathways by binding to recombinational junctions and catalyzing strand cleavage and branch migration. [More information is available at EcoCyc: RUVB].
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Sequence:
NC_000913.3 (1944346..1945356, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene Zn(2(+)) ABC transporter periplasmic binding protein Neighboring gene Zn(2(+)) ABC transporter ATP binding subunit Neighboring gene Zn(2(+)) ABC transporter membrane subunit Neighboring gene Holliday junction branch migration complex subunit RuvA Neighboring gene uncharacterized protein YobI

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Mutational analysis of the functional motifs of RuvB, an AAA+ class helicase and motor protein for holliday junction branch migration. Iwasaki H, et al. Mol Microbiol, 2000 May. PMID 10844644
  2. Overproduction, purification, and ATPase activity of the Escherichia coli RuvB protein involved in DNA repair. Iwasaki H, et al. J Bacteriol, 1989 Oct. PMID 2529252, Free PMC Article
  3. RuvB protein-mediated ATP hydrolysis: functional asymmetry in the RuvB hexamer. Marrione PE, et al. Biochemistry, 1995 Aug 1. PMID 7626650
  4. Direct evidence that a conserved arginine in RuvB AAA+ ATPase acts as an allosteric effector for the ATPase activity of the adjacent subunit in a hexamer. Hishida T, et al. Proc Natl Acad Sci U S A, 2004 Jun 29. PMID 15210950, Free PMC Article
  5. Allosteric effects of RuvA protein, ATP, and DNA on RuvB protein-mediated ATP hydrolysis. Marrione PE, et al. Biochemistry, 1996 Aug 27. PMID 8780528

See all (92) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. The rarA gene as part of an expanded RecFOR recombination pathway: Negative epistasis and synthetic lethality with ruvB, recG, and recQ.
    Title: The rarA gene as part of an expanded RecFOR recombination pathway: Negative epistasis and synthetic lethality with ruvB, recG, and recQ.
  2. Regression of replication forks stalled by leading-strand template damage: both RecG and RuvAB catalyze regression, but RuvC cleaves the holliday junctions formed by RecG preferentially.
    Title: Regression of replication forks stalled by leading-strand template damage: I. Both RecG and RuvAB catalyze regression, but RuvC cleaves the holliday junctions formed by RecG preferentially.
  3. RNA polymerase mutations reduce the synergism between priB and ruvABC.
    Title: Modulation of DNA damage tolerance in Escherichia coli recG and ruv strains by mutations affecting PriB, the ribosome and RNA polymerase.
  4. DNA fragmentation absolutely depends on both RecA-catalyzed homologous strand exchange and RuvABC-catalyzed Holliday junction resolution
    Title: Replication forks stalled at ultraviolet lesions are rescued via RecA and RuvABC protein-catalyzed disintegration in Escherichia coli.
  5. Study hypothesized that RuvAB catalyzes replication fork reversal in order to produce SOS(Con) expression.
    Title: RecA4142 causes SOS constitutive expression by loading onto reversed replication forks in Escherichia coli K-12.
  6. The authors present here the isolation and characterization of ruvA and ruvB single mutants that are impaired for replication fork reversal at forks arrested by the inactivation of polymerase III, while they remain capable of homologous recombination.
    Title: ruvA and ruvB mutants specifically impaired for replication fork reversal.
  7. RuvABC is required to resolve junctions that arise during the repair of a subset of nonarresting lesions after replication has passed through the template
    Title: RuvABC is required to resolve holliday junctions that accumulate following replication on damaged templates in Escherichia coli.
Submit: New GeneRIF Correction
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables ATP hydrolysis activity IEA
Inferred from Electronic Annotation
more info
  
enables DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables DNA helicase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables four-way junction DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables four-way junction helicase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables four-way junction helicase activity IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables nucleotide binding IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in DNA damage response IEA
Inferred from Electronic Annotation
more info
  
involved_in DNA recombination IEA
Inferred from Electronic Annotation
more info
  
involved_in DNA repair IEA
Inferred from Electronic Annotation
more info
  
involved_in SOS response IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within SOS response IEP
Inferred from Expression Pattern
more info
 PubMed 
acts_upstream_of_or_within SOS response RCA
inferred from Reviewed Computational Analysis
more info
 PubMed 
involved_in recombinational repair IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within recombinational repair IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in recombinational repair NAS
Non-traceable Author Statement
more info
 PubMed 
acts_upstream_of_or_within response to UV IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
part_of Holliday junction helicase complex IPI
Inferred from Physical Interaction
more info
 PubMed 
part_of Holliday junction resolvase complex IDA
Inferred from Direct Assay
more info
 PubMed 
part_of Holliday junction resolvase complex IEA
Inferred from Electronic Annotation
more info
  
part_of Holliday junction resolvase complex IPI
Inferred from Physical Interaction
more info
 PubMed 
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
Preferred Names
Holliday junction branch migration complex subunit RuvB

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    1944346..1945356 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_416374.1 Holliday junction branch migration complex subunit RuvB [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_416374.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A6M9WPV1
    Conserved Domains (1) summary
    PRK00080
    Location:6 → 332
    ruvB; Holliday junction branch migration DNA helicase RuvB
Nucleotide Protein
Heading Accession and Version

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