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phoQ sensor histidine kinase PhoQ [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 946326, updated on 17-Jul-2025
Official Symbol
phoQ
Official Full Name
sensor histidine kinase PhoQ
Primary source
ECOCYC:EG10732
Locus tag
b1129
See related
ASAP:ABE-0003805
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK1115
Summary
PhoPQ has been primarily characterized in Salmonella, with significant differences from Escherichia coli. [More information is available at EcoGene: EG10732]. The subunit structure in vivo is not known. [More information is available at EcoCyc: EG10732].
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Sequence:
NC_000913.3 (1188316..1189776, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene peptidase T Neighboring gene 50S ribosomal protein L16-arginine 3-hydroxylase Neighboring gene DNA-binding transcriptional dual regulator PhoP Neighboring gene ncRNA Neighboring gene adenylosuccinate lyase

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. The small membrane protein MgrB regulates PhoQ bifunctionality to control PhoP target gene expression dynamics. Salazar ME, et al. Mol Microbiol, 2016 Nov. PMID 27447896
  2. Cys-scanning disulfide crosslinking and bayesian modeling probe the transmembrane signaling mechanism of the histidine kinase, PhoQ. Molnar KS, et al. Structure, 2014 Sep 2. PMID 25087511, Free PMC Article
  3. Transmembrane polar interactions are required for signaling in the Escherichia coli sensor kinase PhoQ. Goldberg SD, et al. Proc Natl Acad Sci U S A, 2010 May 4. PMID 20404199, Free PMC Article
  4. Atomic insights into the signaling landscape of E. coli PhoQ histidine kinase from molecular dynamics simulations. Lazaridi S, et al. Sci Rep, 2024 Jul 26. PMID 39085378, Free PMC Article
  5. Determination of the physiological dimer interface of the PhoQ sensor domain. Goldberg SD, et al. J Mol Biol, 2008 Jun 13. PMID 18468622, Free PMC Article

See all (49) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Atomic insights into the signaling landscape of E. coli PhoQ histidine kinase from molecular dynamics simulations.
    Title: Atomic insights into the signaling landscape of E. coli PhoQ histidine kinase from molecular dynamics simulations.
  2. data suggest that MgrB affects the acid resistance of E. coli by modulating the expression of iraM, but not completely through PhoP/Q.
    Title: MgrB affects the acid stress response of Escherichia coli by modulating the expression of iraM.
  3. The small inner membrane protein SafA activates PhoQ in Escherichia coli independently from other known signals that control PhoQ activity.
    Title: Identification of an internal cavity in the PhoQ sensor domain for PhoQ activity and SafA-mediated control.
  4. findings directly link the PhoQ/PhoP two-component system to bacterial osmosensing
    Title: Osmosensing by the bacterial PhoQ/PhoP two-component system.
  5. Both MicA and GcvB bind phoPQ mRNA in vivo and in vitro around the translation initiation region of phoP. Binding of either small RNA is sufficient to inhibit ribosome binding and induce mRNA degradation
    Title: Post-transcriptional control of the Escherichia coli PhoQ-PhoP two-component system by multiple sRNAs involves a novel pairing region of GcvB.
  6. Site-directed mutagenesis studies revealed that R53, within SafA(41-65), was important for the activation of PhoQ, and D179 of the PhoQ sensor domain was required for its activation by SafA.
    Title: The connector SafA interacts with the multi-sensing domain of PhoQ in Escherichia coli.
  7. Taken together, these results demonstrate that a decrease in the oxidizing activity of the periplasm stimulates PhoQ/PhoP and may reveal a new input stimulus for this important two-component system.
    Title: Perturbation of the oxidizing environment of the periplasm stimulates the PhoQ/PhoP system in Escherichia coli.
  8. MicA directly pairs with phoPQ mRNA in the translation initiation region of phoP and presumably inhibits translation by competing with ribosome binding.
    Title: MicA sRNA links the PhoP regulon to cell envelope stress.
  9. analysis indicated that PhoQ kinase function is dependent on the polarity of Asn202, rather than its precise structure or position in the transmembrane region.
    Title: Transmembrane polar interactions are required for signaling in the Escherichia coli sensor kinase PhoQ.
  10. Expression of MgrR requires the PhoQ/PhoP two-component system.
    Title: A PhoQ/P-regulated small RNA regulates sensitivity of Escherichia coli to antimicrobial peptides.
Submit: New GeneRIF Correction See all GeneRIFs (11)

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables identical protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables kinase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables metal ion binding IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables phosphoprotein phosphatase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables phosphorelay sensor kinase activity IEA
Inferred from Electronic Annotation
more info
  
enables phosphorelay sensor kinase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein kinase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
acts_upstream_of_or_within cellular response to magnesium starvation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within osmosensory signaling via phosphorelay pathway IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in phosphorelay signal transduction system IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within protein autophosphorylation IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within protein autophosphorylation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within signal transduction IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within signal transduction IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
is_active_in plasma membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in plasma membrane IEA
Inferred from Electronic Annotation
more info
  
located_in plasma membrane ISM
Inferred from Sequence Model
more info
 PubMed 
Preferred Names
sensor histidine kinase PhoQ
NP_415647.1

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    1188316..1189776 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_415647.1 sensor histidine kinase PhoQ

    See identical proteins and their annotated locations for NP_415647.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A2W6P6Y6
    Conserved Domains (1) summary
    PRK10815
    Location:3 → 485
    PRK10815; two-component system sensor histidine kinase PhoQ
Nucleotide Protein
Heading Accession and Version

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