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chbF monoacetylchitobiose-6-phosphate hydrolase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 946266, updated on 30-Jul-2025
Official Symbol
chbF
Official Full Name
monoacetylchitobiose-6-phosphate hydrolase
Primary source
ECOCYC:EG10144
Locus tag
b1734
See related
ASAP:ABE-0005785
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
celF; ECK1732; ydjD
Summary
The chb operon is induced by N,N'-diacetylchitobiose. [More information is available at EcoGene: EG10144]. ChbF is a diacetylchitobiose-6-phosphate hydrolase responsible for converting diacetylchitobiose-6-phosphate to N-acetyl-D-glucosamine and N-acetyl-D-glucosamine-6-phosphate . [More information is available at EcoCyc: EG10144].
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Sequence:
NC_000913.3 (1817148..1818500, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene catalase HPII Neighboring gene chitooligosaccharide monodeacetylase ChbG Neighboring gene DNA binding transcriptional dual regulator ChbR Neighboring gene N,N'-diacetylchitobiose-specific PTS enzyme IIA component

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Chitin, Chitin Oligosaccharide, and Chitin Disaccharide Metabolism of Escherichia coli Revisited: Reassignment of the Roles of ChiA, ChbR, ChbF, and ChbG. Walter A, et al. Microb Physiol, 2021. PMID 33794535
  2. A luxAB transcriptional fusion to the cryptic celF gene of Escherichia coli displays increased luminescence in the presence of nickel. Guzzo A, et al. Mol Gen Genet, 1994 Feb. PMID 8121401
  3. Mutations that alter the regulation of the chb operon of Escherichia coli allow utilization of cellobiose. Kachroo AH, et al. Mol Microbiol, 2007 Dec. PMID 18028317
  4. Directed evolution of cellobiose utilization in Escherichia coli K12. Kricker M, et al. Mol Biol Evol, 1984 Feb. PMID 6400650
  5. Biochemical genetics of the cryptic gene system for cellobiose utilization in Escherichia coli K12. Kricker M, et al. Genetics, 1987 Mar. PMID 3552873, Free PMC Article

See all (12) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. N-terminus verified by Edman degradation on mature peptide
    Title: Cellobiose-6-phosphate hydrolase (CelF) of Escherichia coli: characterization and assignment to the unusual family 4 of glycosylhydrolases.
Submit: New GeneRIF Correction

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables 6-phospho-beta-glucosidase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables 6-phospho-beta-glucosidase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables 6-phospho-beta-glucosidase activity IEA
Inferred from Electronic Annotation
more info
  
enables catalytic activity IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity, acting on glycosyl bonds IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity, hydrolyzing O-glycosyl compounds IEA
Inferred from Electronic Annotation
more info
  
enables identical protein binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in carbohydrate metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in diacetylchitobiose catabolic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
Preferred Names
monoacetylchitobiose-6-phosphate hydrolase
NP_416248.1

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    1817148..1818500 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_416248.1 monoacetylchitobiose-6-phosphate hydrolase

    See identical proteins and their annotated locations for NP_416248.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A376S8U2, A0A8S7SZ02
    Conserved Domains (1) summary
    cd05296
    Location:5 → 436
    GH4_P_beta_glucosidase; Glycoside Hydrolases Family 4; Phospho-beta-glucosidase
Nucleotide Protein
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