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rluA 23S rRNA pseudouridine(746) and tRNA pseudouridine(32) synthase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 946262, updated on 19-Nov-2025
Official Symbol
rluA
Official Full Name
23S rRNA pseudouridine(746) and tRNA pseudouridine(32) synthase
Primary source
ECOCYC:EG12609
Locus tag
b0058
See related
ASAP:ABE-0000196
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK0059; yabO
Summary
RluA is the pseudouridine synthase that catalyzes formation of pseudouridine at position 746 in 23S rRNA and at position 32 in tRNAPhe . [More information is available at EcoCyc: EG12609].
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Sequence:
NC_000913.3 (59687..60346, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene co-chaperone protein DjlA Neighboring gene putative uncharacterized protein YabP Neighboring gene protein YabQ Neighboring gene RNA polymerase-binding ATPase and RNAP recycling factor Neighboring gene DNA polymerase II

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Crystal structure of pseudouridine synthase RluA: indirect sequence readout through protein-induced RNA structure. Hoang C, et al. Mol Cell, 2006 Nov 17. PMID 17188032
  2. Mechanistic investigations of the pseudouridine synthase RluA using RNA containing 5-fluorouridine. Hamilton CS, et al. Biochemistry, 2006 Oct 3. PMID 17002302, Free PMC Article
  3. Artificial polypeptide scaffold for protein immobilization. Zhang K, et al. J Am Chem Soc, 2005 Jul 27. PMID 16028902
  4. Purification, structure, and properties of Escherichia coli tRNA pseudouridine synthase I. Kammen HO, et al. J Biol Chem, 1988 Feb 15. PMID 3276686
  5. A frequently amplified region in Leishmania contains a gene conserved in prokaryotes and eukaryotes. Myler PJ, et al. Gene, 1994 Oct 21. PMID 7958944

See all (23) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. RluA displayed a rate constant of pseudouridine formation of 0.7 sec(-1).
    Title: Pre-steady-state kinetic analysis of the three Escherichia coli pseudouridine synthases TruB, TruA, and RluA reveals uniformly slow catalysis.
  2. N-terminus verified by Edman degradation on mature peptide
    Title: A dual-specificity pseudouridine synthase: an Escherichia coli synthase purified and cloned on the basis of its specificity for psi 746 in 23S RNA is also specific for psi 32 in tRNA(phe).
  3. The 2.05 A resolution structure of RluA bound to a substrate RNA comprising the anticodon stem loop of tRNA(Phe) reveals that enzyme binding induces a dramatic reorganization of the RNA.
    Title: Crystal structure of pseudouridine synthase RluA: indirect sequence readout through protein-induced RNA structure.
Submit: New GeneRIF Correction
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables 23S rRNA pseudouridine(746) synthase activity IEA
Inferred from Electronic Annotation
more info
  
enables RNA binding IEA
Inferred from Electronic Annotation
more info
  
enables catalytic activity, acting on RNA IEA
Inferred from Electronic Annotation
more info
  
enables isomerase activity IEA
Inferred from Electronic Annotation
more info
  
enables pseudouridine synthase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables pseudouridine synthase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables pseudouridine synthase activity IEA
Inferred from Electronic Annotation
more info
  
enables pseudouridine synthase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables rRNA pseudouridine synthase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables tRNA pseudouridine synthase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables tRNA pseudouridine(32) synthase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in RNA modification IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within RNA modification IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in RNA processing IEA
Inferred from Electronic Annotation
more info
  
involved_in enzyme-directed rRNA pseudouridine synthesis IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within enzyme-directed rRNA pseudouridine synthesis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in pseudouridine synthesis IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within pseudouridine synthesis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in rRNA processing IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within rRNA processing IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within rRNA pseudouridine synthesis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in tRNA processing IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within tRNA processing IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within tRNA pseudouridine synthesis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
Preferred Names
23S rRNA pseudouridine(746) and tRNA pseudouridine(32) synthase
NP_414600.1

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    59687..60346 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_414600.1 23S rRNA pseudouridine(746) and tRNA pseudouridine(32) synthase

    See identical proteins and their annotated locations for NP_414600.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A2W6PA86
    Conserved Domains (1) summary
    PRK10158
    Location:1 → 219
    PRK10158; bifunctional tRNA pseudouridine(32) synthase/23S rRNA pseudouridine(746) synthase RluA
Nucleotide Protein
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