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metC cystathionine beta-lyase/L-cysteine desulfhydrase/alanine racemase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 946240, updated on 16-Jun-2026
Official Symbol
metC
Official Full Name
cystathionine beta-lyase/L-cysteine desulfhydrase/alanine racemase
Primary source
ECOCYC:EG10583
Locus tag
b3008
See related
ASAP:ABE-0009878
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK3000
Summary
The Flint et al. [More information is available at EcoGene: EG10583]. Several distinct proteins exhibit cysteine desulfhydrase activity, which is involved in cysteine degradation . [More information is available at EcoCyc: EG10583].
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Sequence:
NC_000913.3 (3152236..3153423)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene Ton complex subunit ExbD Neighboring gene Ton complex subunit ExbB Neighboring gene DedA family protein YghB Neighboring gene DNA-binding transcriptional activator YqhC

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Characterization of the side-chain hydroxyl moieties of residues Y56, Y111, Y238, Y338, and S339 as determinants of specificity in E. coli cystathionine β-lyase. Lodha PH, et al. Biochemistry, 2011 Nov 15. PMID 21958132
  2. Cloning, purification, and characterization of beta-cystathionase from Escherichia coli. Dwivedi CM, et al. Biochemistry, 1982 Jun 22. PMID 7049234
  3. Cloning, purification, and crystallization of Escherichia coli cystathionine beta-lyase. Laber B, et al. FEBS Lett, 1996 Jan 22. PMID 8566238
  4. Mode of action of cystathionine beta-lyase. Clausen T, et al. Biol Chem, 1997 Mar-Apr. PMID 9165088
  5. Slow-binding inhibition of Escherichia coli cystathionine beta-lyase by L-aminoethoxyvinylglycine: a kinetic and X-ray study. Clausen T, et al. Biochemistry, 1997 Oct 14. PMID 9376370

See all (26) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. a representative of fold type III, the Escherichia coli alanine racemase (ALR), is a promiscuous cystathionine beta-lyase (CBL). Furthermore, E. coli CBL (fold type I) is a promiscuous alanine racemase. A single round of error-prone PCR and selection yielded variant ALR(Y274F), which catalyzes cystathionine beta-elimination with a near-native Michaelis constant (Km = 3.3 mm) but a poor turnover number (kcat approximate...
    Title: Mechanistic and Evolutionary Insights from the Reciprocal Promiscuity of Two Pyridoxal Phosphate-dependent Enzymes.
  2. A series of site-directed variants of CBL was constructed to investigate the roles of the hydroxyl moieties of active-site residues Y56, Y111, Y238, Y338, and S339 as determinants of specificity.
    Title: Characterization of the side-chain hydroxyl moieties of residues Y56, Y111, Y238, Y338, and S339 as determinants of specificity in E. coli cystathionine β-lyase.
  3. N-terminus verified by Edman degradation on mature peptide
    Title: Studies on the synthesis of the Fe-S cluster of dihydroxy-acid dehydratase in escherichia coli crude extract. Isolation of O-acetylserine sulfhydrylases A and B and beta-cystathionase based on their ability to mobilize sulfur from cysteine and to participate in Fe-S cluster synthesis.
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Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables L-cysteine desulfhydrase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables L-cysteine desulfhydrase activity IEA
Inferred from Electronic Annotation
more info
  
enables L-cysteine desulfhydrase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables alanine racemase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables catalytic activity IEA
Inferred from Electronic Annotation
more info
  
enables cysteine-S-conjugate beta-lyase activity EXP
Inferred from Experiment
more info
 PubMed 
enables cysteine-S-conjugate beta-lyase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables cysteine-S-conjugate beta-lyase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables cysteine-S-conjugate beta-lyase activity IEA
Inferred from Electronic Annotation
more info
  
enables identical protein binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables pyridoxal phosphate binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables pyridoxal phosphate binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in L-cysteine catabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in amino acid metabolic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within protein homotetramerization IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in sulfur amino acid metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in transsulfuration IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
part_of protein-containing complex IDA
Inferred from Direct Assay
more info
 PubMed 
Preferred Names
cystathionine beta-lyase/L-cysteine desulfhydrase/alanine racemase
NP_417481.1

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    3152236..3153423
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_417481.1 cystathionine beta-lyase/L-cysteine desulfhydrase/alanine racemase

    See identical proteins and their annotated locations for NP_417481.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A0H2Z2V7
    Conserved Domains (1) summary
    PRK08114
    Location:1 → 395
    PRK08114; cystathionine beta-lyase; Provisional
Nucleotide Protein
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