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infC [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 946225, updated on 11-Sep-2024

Summary

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Official Symbol
infC
Primary source
ECOCYC:EG10506
Locus tag
b1718
See related
ASAP:ABE-0005734
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK1716; fit; srjA
Summary
HT_Cmplx17_Mem: [More information is available at EcoGene: EG10506]. IF-3 is one of three translation initiation factors in Escherichia coli . [More information is available at EcoCyc: EG10506].
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Genomic context

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Sequence:
NC_000913.3 (1800096..1800638, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene 50S ribosomal subunit protein L20 Neighboring gene 50S ribosomal subunit protein L35 Neighboring gene threonine--tRNA ligase Neighboring gene pseudo

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. The initiation factor 3 (IF3) residues interacting with initiator tRNA elbow modulate the fidelity of translation initiation and growth fitness in Escherichia coli. Singh J, et al. Nucleic Acids Res, 2022 Nov 11. PMID 36399509, Free PMC Article
  2. Ribosome assembly defects subvert initiation Factor3 mediated scrutiny of bona fide start signal. Sharma H, et al. Nucleic Acids Res, 2019 Dec 2. PMID 31586395, Free PMC Article
  3. Conformational selection of translation initiation factor 3 signals proper substrate selection. Elvekrog MM, et al. Nat Struct Mol Biol, 2013 May. PMID 23584454, Free PMC Article
  4. Control of translation initiation involves a factor-induced rearrangement of helix 44 of 16S ribosomal RNA. Qin D, et al. Mol Microbiol, 2009 Mar. PMID 19154330, Free PMC Article
  5. A single mutation in the IF3 N-terminal domain perturbs the fidelity of translation initiation at three levels. Maar D, et al. J Mol Biol, 2008 Nov 28. PMID 18805426

See all (89) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. The initiation factor 3 (IF3) residues interacting with initiator tRNA elbow modulate the fidelity of translation initiation and growth fitness in Escherichia coli.
    Title: The initiation factor 3 (IF3) residues interacting with initiator tRNA elbow modulate the fidelity of translation initiation and growth fitness in Escherichia coli.
  2. The premature ribosomes harbouring the assembly defects subvert the IF3 mediated proofreading of cognate initiation codon to enter the translation cycle.
    Title: Ribosome assembly defects subvert initiation Factor3 mediated scrutiny of bona fide start signal.
  3. By gating the transition from 30S ribosome biogenesis to translation initiation, RbfA and IF3 maintain the fidelity of bacterial protein synthesis.
    Title: RbfA and IF3 couple ribosome biogenesis and translation initiation to increase stress tolerance.
  4. The C-terminal domain of infC is essential for growth and the N-terminal domain adds to the fidelity of initiation of protein synthesis and to the fitness of the bacterial growth.
    Title: Contributions of the N- and C-Terminal Domains of Initiation Factor 3 to Its Functions in the Fidelity of Initiation and Antiassociation of the Ribosomal Subunits.
  5. Data show that bacterial initiation factor IF2 accelerated ribosome subunit joining, whereas bacterial initiation factors IF1 and IF3 significantly slowed down subunit association in the absence of IF2.
    Title: Initiation factor 2 stabilizes the ribosome in a semirotated conformation.
  6. These results strongly support a conformational selection model in which the conformation of IF3 that is selectively stabilized within a completely and correctly assembled 30S IC facilitates further progress along the initiation pathway.
    Title: Conformational selection of translation initiation factor 3 signals proper substrate selection.
  7. Ribosomes change into forms which are hard to bind with IF3 and easy to form 100S ribosomes by RMF and HPF concomitantly with transition from the log phase to the stationary phase.
    Title: Activities of Escherichia coli ribosomes in IF3 and RMF change to prepare 100S ribosome formation on entering the stationary growth phase.
  8. overexpression of IF3 led to a marked decrease in re-initiation efficiency, suggesting that a 30S ribosome and not a 70S ribosome is used for translation re-initiation
    Title: Requirements for translation re-initiation in Escherichia coli: roles of initiator tRNA and initiation factors IF2 and IF3.
  9. N-terminus verified by Edman degradation on complete protein
    Title: The primary structure of the initiation factor IF-3 from Escherichia coli.
  10. Study shows that after cold shock two promoters are activated resulting in de novo infC transcription and IF3 synthesis resulting in IF3/ribosomes ratio that contributes to establishing the cold-shock translational bias.
    Title: Cold-stress-induced de novo expression of infC and role of IF3 in cold-shock translational bias.
Submit: New GeneRIF Correction See all GeneRIFs (12)

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables RNA binding IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables ribosome binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables ribosome binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables translation initiation factor activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables translation initiation factor activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables translation initiation factor activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
acts_upstream_of_or_within response to cold IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in ribosome disassembly IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within ribosome disassembly IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in translation IEA
Inferred from Electronic Annotation
more info
  
involved_in translational initiation IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
located_in membrane HDA PubMed 

General protein information

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Preferred Names
translation initiation factor IF-3

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    1800096..1800638 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_416233.1 translation initiation factor IF-3 [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_416233.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A5B9AIA3
    Conserved Domains (1) summary
    PRK00028
    Location:5 → 179
    infC; translation initiation factor IF-3; Reviewed
Nucleotide Protein
Heading Accession and Version

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