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entB enterobactin synthase component B [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 946178, updated on 6-Jul-2026
Official Symbol
entB
Official Full Name
enterobactin synthase component B
Primary source
ECOCYC:EG10260
Locus tag
b0595
See related
ASAP:ABE-0002052
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK0588; entG
Summary
Recent gel filtration data suggest that EntB may be a trimer not a pentamer. [More information is available at EcoCyc: EG10260].
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Sequence:
NC_000913.3 (627694..628551)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene isochorismate synthase EntC Neighboring gene 2,3-dihydroxybenzoate-AMP ligase Neighboring gene 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase Neighboring gene proofreading thioesterase in enterobactin biosynthesis Neighboring gene pyruvate transporter CstA

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Structural and functional investigation of the intermolecular interaction between NRPS adenylation and carrier protein domains. Sundlov JA, et al. Chem Biol, 2012 Feb 24. PMID 22365602, Free PMC Article
  2. Subcloning of the enterobactin biosynthetic gene entB: expression, purification, characterization, and substrate specificity of isochorismatase. Rusnak F, et al. Biochemistry, 1990 Feb 13. PMID 2139796
  3. EntG activity of Escherichia coli enterobactin synthetase. Staab JF, et al. J Bacteriol, 1990 Nov. PMID 2172214, Free PMC Article
  4. Ligand-induced conformational rearrangements promote interaction between the Escherichia coli enterobactin biosynthetic proteins EntE and EntB. Khalil S, et al. J Mol Biol, 2009 Oct 30. PMID 19699210
  5. Membrane association of the Escherichia coli enterobactin synthase proteins EntB/G, EntE, and EntF. Hantash FM, et al. J Bacteriol, 2000 Mar. PMID 10692387, Free PMC Article

See all (27) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. The aryl carrier protein (ArCP) domain of EntB interacts specifically with EntH to help with the proofreading role for EntH during biosynthesis of enterobactin.
    Title: The hotdog thioesterase EntH (YbdB) plays a role in vivo in optimal enterobactin biosynthesis by interacting with the ArCP domain of EntB.
  2. crystal structure of the EntB protein that contains an N-terminal isochorismate lyase domain that functions in the synthesis of 2,3-dihydroxybenzoate and a C-terminal carrier protein domain
    Title: Structure of the EntB multidomain nonribosomal peptide synthetase and functional analysis of its interaction with the EntE adenylation domain.
Submit: New GeneRIF Correction
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables 2,3-dihydroxybenzoate-serine ligase activity EXP
Inferred from Experiment
more info
 PubMed 
enables 2,3-dihydroxybenzoate-serine ligase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables 2,3-dihydroxybenzoate-serine ligase activity IEA
Inferred from Electronic Annotation
more info
  
enables identical protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables isochorismatase activity EXP
Inferred from Experiment
more info
 PubMed 
enables isochorismatase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables isochorismatase activity IEA
Inferred from Electronic Annotation
more info
  
enables magnesium ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables phosphopantetheine binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables transferase activity, transferring alkyl or aryl (other than methyl) groups IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Process Evidence Code Pubs
involved_in enterobactin biosynthetic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in enterobactin biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytoplasm EXP
Inferred from Experiment
more info
 PubMed 
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
located_in plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
Preferred Names
enterobactin synthase component B
NP_415127.1

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    627694..628551
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_415127.1 enterobactin synthase component B

    See identical proteins and their annotated locations for NP_415127.1

    Status: REVIEWED

    Conserved Domains (2) summary
    COG1535
    Location:1 → 215
    EntB; Isochorismate hydrolase [Secondary metabolites biosynthesis, transport and catabolism]
    COG3433
    Location:216 → 285
    DhbB2; Aryl carrier domain [Secondary metabolites biosynthesis, transport and catabolism]
Nucleotide Protein
Heading Accession and Version

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