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uidA beta-glucuronidase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 946149, updated on 30-Jul-2025
Official Symbol
uidA
Official Full Name
beta-glucuronidase
Primary source
ECOCYC:EG11055
Locus tag
b1617
See related
ASAP:ABE-0005410
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK1612; gurA; gusA
Summary
UidA has an Ig-like domain: pfam PF00703 aligns to residues 182-272. ebgA, uidA and lacZ are paralogs. [More information is available at EcoGene: EG11055]. D-glucuronidase catalyzes the cleavage of a wide variety of -glucuronides . -D-glucuronidase and |FRAME: [More information is available at EcoCyc: EG11055].
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Sequence:
NC_000913.3 (1694260..1696071, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene DUF945 domain-containing protein YdgA Neighboring gene outer membrane porin family protein UidC Neighboring gene glucuronide:H(+) symporter Neighboring gene DNA-binding transcriptional repressor UidR Neighboring gene 7-alpha-hydroxysteroid dehydrogenase

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. [On the properties and the biosynthesis of beta-glucuronidase of Escherichia coli K 12]. Didier-Fichet ML, et al. C R Acad Hebd Seances Acad Sci D, 1968 May 6. PMID 4970455
  2. A comparison of directed evolution approaches using the beta-glucuronidase model system. Rowe LA, et al. J Mol Biol, 2003 Sep 26. PMID 12972256
  3. Flow cytometric detection of beta-D-glucuronidase gene in wild-type bacterial cells using in-situ PCR. Sachidanandham R, et al. Biotechnol Bioeng, 2003 Apr 20. PMID 12584754
  4. Drug-Encoded Biomarkers for Monitoring Biological Therapies. Tsoneva D, et al. PLoS One, 2015. PMID 26348361, Free PMC Article
  5. Expression and purification of Escherichia coli beta-glucuronidase. Aich S, et al. Protein Expr Purif, 2001 Jun. PMID 11388802

See all (40) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. We found GusA to be the most reliable biomarker for monitoring oncolytic virotherapy among the three tested markers
    Title: Drug-Encoded Biomarkers for Monitoring Biological Therapies.
  2. there is not only an active dimer of the E. coli form of beta-glucuronidase, but also several larger active complexes, including an octomer and a 16-mer
    Title: Native Electrophoresis-Coupled Activity Assays Reveal Catalytically-Active Protein Aggregates of Escherichia coli β-Glucuronidase.
  3. we performed in vitro directed evolution & obtained a mutant GUS that was highly thermostable. mutation & reversion approaches identified 6 key AAs (Q493R, T509A, M532T, N550S, G559S and N566S) that are responsible for the improved thermostability
    Title: Concurrent mutations in six amino acids in beta-glucuronidase improve its thermostability.
Submit: New GeneRIF Correction

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables beta-glucuronidase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables beta-glucuronidase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables beta-glucuronidase activity IEA
Inferred from Electronic Annotation
more info
  
enables carbohydrate binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity, acting on glycosyl bonds IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity, hydrolyzing O-glycosyl compounds IEA
Inferred from Electronic Annotation
more info
  
enables identical protein binding IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in carbohydrate metabolic process IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
part_of protein-containing complex IDA
Inferred from Direct Assay
more info
 PubMed 
Preferred Names
beta-glucuronidase
NP_416134.1

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    1694260..1696071 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_416134.1 beta-glucuronidase

    See identical proteins and their annotated locations for NP_416134.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A8I0CCI6
    Conserved Domains (1) summary
    PRK10150
    Location:1 → 600
    PRK10150; beta-D-glucuronidase; Provisional
Nucleotide Protein
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