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arfB peptidyl-tRNA hydrolase, ribosome rescue factor [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 946046, updated on 23-Apr-2026
Official Symbol
arfB
Official Full Name
peptidyl-tRNA hydrolase, ribosome rescue factor
Primary source
ECOCYC:EG12354
Locus tag
b0191
See related
ASAP:ABE-0000650
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK0191; yaeJ
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Sequence:
NC_000913.3 (214833..215255)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene tRNA(Ile)-lysidine synthetase Neighboring gene modulator of Rho-dependent transcription termination Neighboring gene PF06786 family protein YaeP Neighboring gene uncharacterized protein YaeQ Neighboring gene sensor lipoprotein NlpE Neighboring gene peptidase C92 family protein YaeF

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Structural basis for the rescue of stalled ribosomes: structure of YaeJ bound to the ribosome. Gagnon MG, et al. Science, 2012 Mar 16. PMID 22422986, Free PMC Article
  2. Escherichia coli YaeJ protein mediates a novel ribosome-rescue pathway distinct from SsrA- and ArfA-mediated pathways. Chadani Y, et al. Mol Microbiol, 2011 May. PMID 21418110
  3. YaeJ is a novel ribosome-associated protein in Escherichia coli that can hydrolyze peptidyl-tRNA on stalled ribosomes. Handa Y, et al. Nucleic Acids Res, 2011 Mar. PMID 21051357, Free PMC Article
  4. Overproduction of NlpE, a new outer membrane lipoprotein, suppresses the toxicity of periplasmic LacZ by activation of the Cpx signal transduction pathway. Snyder WB, et al. J Bacteriol, 1995 Aug. PMID 7635808, Free PMC Article
  5. ArfA recruits RF2 into stalled ribosomes. Shimizu Y. J Mol Biol, 2012 Nov 2. PMID 22922063

See all (10) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. the efficiency of the ArfA-dependent process decreases rapidly with an increase in mRNA length downstream of the A site of the ribosome whereas YaeJ function is maintained on mRNA with sufficient length.
    Title: ArfA recruits RF2 into stalled ribosomes.
  2. 3.2 A crystal structure of YaeJ bound to 70S ribosome in complex with the initiator tRNA(i)(fMet) and a short mRNA; structure reveals the C-terminal tail of YaeJ functions as a sensor to discriminate between stalled and actively translating ribosomes
    Title: Structural basis for the rescue of stalled ribosomes: structure of YaeJ bound to the ribosome.
  3. In this study, the authors identified the E. coli yaeJ gene, encoding a peptide-release factor homologue with GGQ motif, as a multicopy suppressor of the lethal phenotype of ssrA arfA double mutant.
    Title: Escherichia coli YaeJ protein mediates a novel ribosome-rescue pathway distinct from SsrA- and ArfA-mediated pathways.
  4. Results suggest that YaeJ functions as a ribosome-attached rescue device for stalled ribosomes.
    Title: YaeJ is a novel ribosome-associated protein in Escherichia coli that can hydrolyze peptidyl-tRNA on stalled ribosomes.
Submit: New GeneRIF Correction

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables peptidyl-tRNA hydrolase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables peptidyl-tRNA hydrolase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables peptidyl-tRNA hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables ribosome binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables ribosome binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables translation release factor activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in rescue of stalled cytosolic ribosome IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within rescue of stalled cytosolic ribosome IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in translational termination IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
Preferred Names
peptidyl-tRNA hydrolase, ribosome rescue factor
NP_414733.1

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    214833..215255
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_414733.1 peptidyl-tRNA hydrolase, ribosome rescue factor

    See identical proteins and their annotated locations for NP_414733.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A376RQK5
    Conserved Domains (1) summary
    PRK09256
    Location:1 → 136
    PRK09256; aminoacyl-tRNA hydrolase
Nucleotide Protein
Heading Accession and Version

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