U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

paaF putative 2,3-dehydroadipyl-CoA hydratase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 946011, updated on 26-Mar-2025
Official Symbol
paaF
Official Full Name
putative 2,3-dehydroadipyl-CoA hydratase
Primary source
ECOCYC:G6714
Locus tag
b1393
See related
ASAP:ABE-0004659
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK1390; ydbR; ydbS
Summary
paaF mutants accumulate delta3-dehydroadipate. [More information is available at EcoGene: EG13740]. PaaF is a putative enoyl-CoA hydratase/isomerase involved in phenylacetate catabolism . [More information is available at EcoCyc: G6714].
NEW
Try the new Gene table
Try the new Transcript table
Sequence:
NC_000913.3 (1457497..1458264)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene phenylacetyl-CoA 1,2-epoxidase, structural subunit Neighboring gene phenylacetate degradation protein Neighboring gene phenylacetyl-CoA 1,2-epoxidase, reductase subunit Neighboring gene putative ring 1,2-epoxyphenylacetyl-CoA isomerase (oxepin-CoA forming) Neighboring gene 3-hydroxyadipyl-CoA dehydrogenase Neighboring gene phenylacetyl-CoA thioesterase

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Protein-protein interactions in the β-oxidation part of the phenylacetate utilization pathway: crystal structure of the PaaF-PaaG hydratase-isomerase complex. Grishin AM, et al. J Biol Chem, 2012 Nov 2. PMID 22961985, Free PMC Article
  2. New FadB homologous enzymes and their use in enhanced biosynthesis of medium-chain-length polyhydroxyalkanoates in FadB mutant Escherichia coli. Park SJ, et al. Biotechnol Bioeng, 2004 Jun 20. PMID 15137080
  3. Functional genomics by NMR spectroscopy. Phenylacetate catabolism in Escherichia coli. Ismail W, et al. Eur J Biochem, 2003 Jul. PMID 12846838
  4. Bacterial phenylalanine and phenylacetate catabolic pathway revealed. Teufel R, et al. Proc Natl Acad Sci U S A, 2010 Aug 10. PMID 20660314, Free PMC Article
  5. Transcriptional regulation of the divergent paa catabolic operons for phenylacetic acid degradation in Escherichia coli. Ferrández A, et al. J Biol Chem, 2000 Apr 21. PMID 10766858

See all (10) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Data indicate that the PaaFG complex has a four-layered structure composed of homotrimeric discs of PaaF and PaaG.
    Title: Protein-protein interactions in the β-oxidation part of the phenylacetate utilization pathway: crystal structure of the PaaF-PaaG hydratase-isomerase complex.
Submit: New GeneRIF Correction

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables catalytic activity IEA
Inferred from Electronic Annotation
more info
  
enables enoyl-CoA hydratase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables enoyl-CoA hydratase activity IEA
Inferred from Electronic Annotation
more info
  
enables identical protein binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables lyase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in fatty acid beta-oxidation IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in fatty acid metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in lipid metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in phenylacetate catabolic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within phenylacetate catabolic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in phenylacetate catabolic process NAS
Non-traceable Author Statement
more info
 PubMed 
Component Evidence Code Pubs
part_of catalytic complex IPI
Inferred from Physical Interaction
more info
 PubMed 
Preferred Names
putative 2,3-dehydroadipyl-CoA hydratase

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    1457497..1458264
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_415911.1 putative 2,3-dehydroadipyl-CoA hydratase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_415911.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A776LQI6
    Conserved Domains (1) summary
    PRK09674
    Location:1 → 255
    PRK09674; enoyl-CoA hydratase-isomerase; Provisional
Nucleotide Protein
Heading Accession and Version

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.