dmsD redox enzyme maturation protein DmsD [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Gene symbol: dmsD
Gene description: redox enzyme maturation protein DmsD
Primary source: ASAP:ABE-0005314
Locus tag: b1591
See related: ECOCYC:G6849
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: ECK1586; ynfI
Summary: dmsD is the fifth gene in the dmsABC-like operon ynfEFGH-dmsD. torD, dmsD and ycdY are paralogs.Overexpressed YdcY and DmsD interfere with apoTorA maturation by TorD, independent of the TorA signal peptide (Ilbert, 2004). [More information is available at EcoGene: EG13847]. DmsD binds to the twin-arginine leaders of certain proteins, including dimethylsulfoxide (DMSO) reductase and trimethylamine N-oxide (TMAO) reductase that are translocated to the cytoplasmic membrane for the periplasm by the Sec-independent system that is termed Mtt (membrane targeting and transport) or more frequently Tat (twin-arginine translocation) . [More information is available at EcoCyc: G6849].
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Genomic context

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Sequence:: NC_000913.3 (1664506..1665120)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

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Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

utilized the in vitro protein-protein interaction technique of an affinity pull down assay, as well as protein thermal stability measurement via differential scanning fluorimetry to investigate the interaction of guanosine nucleotides with DmsD
Title: Influence of GTP on system specific chaperone - Twin arginine signal peptide interaction.
Data suggest that a portion of the hydrophobic region of the DmsA (dimethyl sulfoxide reductase, subunit A) leader peptide determines the specificity of binding and may produce helical properties upon binding to DmsD.
Title: The hydrophobic region of the DmsA twin-arginine leader peptide determines specificity with chaperone DmsD.
analysis of DmsD (specific for dimethylsulfoxide reductase, DmsA) and TorD (specific for trimethylamine N-oxide reductase, TorA).
Title: The hydrophobic core of twin-arginine signal sequences orchestrates specific binding to Tat-pathway related chaperones.
DmsD protein is required for reduction of selenate.
Title: A genetic analysis of in vivo selenate reduction by Salmonella enterica serovar Typhimurium LT2 and Escherichia coli K12.
Results illustrate the positions of important DmsD residues involved in binding the DmsA leader peptide and identify a "hot pocket" of residues important for leader binding on the structure of DmsD.
Title: Identification of residues in DmsD for twin-arginine leader peptide binding, defined through random and bioinformatics-directed mutagenesis.
N-terminus verified by Edman degradation on mature peptide
Title: Identification of a twin-arginine leader-binding protein.

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General gene information

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Gene Ontology Provided by GO

Function	Evidence Code	Pubs
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables signal sequence binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within chaperone-mediated protein folding	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	IMP Inferred from Mutant Phenotype more info	PubMed
located_in extrinsic component of cytoplasmic side of plasma membrane	IMP Inferred from Mutant Phenotype more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: redox enzyme maturation protein DmsD

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_000913.3 Reference assembly

Range

1664506..1665120

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NP_416108.2 redox enzyme maturation protein DmsD [Escherichia coli str. K-12 substr. MG1655]

See identical proteins and their annotated locations for NP_416108.2

Status: PROVISIONAL

UniProtKB/Swiss-Prot

P69853

Conserved Domains (1) summary

PRK11621
Location:1 → 204

PRK11621; Tat proofreading chaperone DmsD