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prpD 2-methylcitrate dehydratase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 945931, updated on 8-Apr-2026
Official Symbol
prpD
Official Full Name
2-methylcitrate dehydratase
Primary source
ECOCYC:G6199
Locus tag
b0334
See related
ASAP:ABE-0001152
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
acnC; ECK0331; yahT
Summary
Crp regulon. [More information is available at EcoGene: EG13603]. The prpD gene encodes 2-methylcitrate dehydratase activity and also encodes the minor aconitase activity, aconitase C , which constitutes 5% or less of cellular activity and is observed in an acnA acnB double mutant . [More information is available at EcoCyc: G6199].
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Sequence:
NC_000913.3 (351215..352666)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene 2-methylisocitrate lyase Neighboring gene 2-methylcitrate synthase Neighboring gene propionyl-CoA synthetase Neighboring gene cytosine transporter Neighboring gene cytosine/isoguanine deaminase

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Construction and properties of aconitase mutants of Escherichia coli. Gruer MJ, et al. Microbiology (Reading), 1997 Jun. PMID 9202458
  2. Propionate catabolism in Salmonella typhimurium LT2: two divergently transcribed units comprise the prp locus at 8.5 centisomes, prpR encodes a member of the sigma-54 family of activators, and the prpBCDE genes constitute an operon. Horswill AR, et al. J Bacteriol, 1997 Feb. PMID 9006051, Free PMC Article
  3. Salmonella typhimurium LT2 catabolizes propionate via the 2-methylcitric acid cycle. Horswill AR, et al. J Bacteriol, 1999 Sep. PMID 10482501, Free PMC Article
  4. Propionate oxidation in Escherichia coli: evidence for operation of a methylcitrate cycle in bacteria. Textor S, et al. Arch Microbiol, 1997 Nov. PMID 9325432
  5. Temporal gene-expression in Escherichia coli K-12 biofilms. Domka J, et al. Environ Microbiol, 2007 Feb. PMID 17222132

See all (14) citations in PubMed

GeneRIFs: Gene References Into Functions

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables 2 iron, 2 sulfur cluster binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables 2 iron, 2 sulfur cluster binding IEA
Inferred from Electronic Annotation
more info
  
enables 2-methylcitrate dehydratase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables 2-methylcitrate dehydratase activity IEA
Inferred from Electronic Annotation
more info
  
enables aconitate hydratase activity IEA
Inferred from Electronic Annotation
more info
  
enables lyase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in propionate metabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in propionate metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in tricarboxylic acid cycle IEA
Inferred from Electronic Annotation
more info
  
Preferred Names
2-methylcitrate dehydratase
NP_414868.1

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    351215..352666
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_414868.1 2-methylcitrate dehydratase

    See identical proteins and their annotated locations for NP_414868.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A8S7XFU9, A0AAD2ZCA6
    Conserved Domains (1) summary
    PRK09425
    Location:1 → 483
    prpD; bifunctional 2-methylcitrate dehydratase/aconitate hydratase
Nucleotide Protein
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